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The importance of precaution in the lab
Importance of safety in the laboratory
Flashcards.safety in the science.laboratory
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Recommended: The importance of precaution in the lab
Laboratory Procedures
Objective:
1. To learn proper safety techniques with laboratory equipment.
2. To learn the names and functions of all items in the lab.
3. To learn to use all lab materials safely and effectively.
Procedure:
Part 1-The Burner - Familiarize yourself with the gas burner. Recognize all parts and learn how to operate them properly. Learn how to safely light the gas burner, and control the flame. Clean up the lab station when finished.
Part 2- Glass Manipulations - Educate yourself on how to properly insert glass tubing into rubber stoppers. Also note the proper safety precautions that are necessary to do so. Lastly, learn how to safely insert glass tubing into a plastic hose. Clean lab station.
Part 3- Handling Solids- Note how to properly retrieve solid chemicals out of their container. Learn all safety precautions that should be taken when handling solid chemicals. Teach yourself how to transfer solid chemicals safely. Clean lab station.
Part 4- The Balance - Make sure the balance is set up properly...
The unknown bacterium that was handed out by the professor labeled “E19” was an irregular and raised shaped bacteria with a smooth texture and it had a white creamy color. The slant growth pattern was filiform and there was a turbid growth in the broth. After all the tests were complete and the results were compared the unknown bacterium was defined as Shigella sonnei. The results that narrowed it down the most were the gram stain, the lactose fermentation test, the citrate utilization test and the indole test. The results for each of the tests performed are listed in Table 1.1 below.
There were minimal risks in this section of the practical as glucose is a sugar and is not harmful; however we still wore lab coats and goggles to protect our clothing and eyes from any accidental splashing. We also had to take care when using the pipettes so not to stab ourselves or anyone with them so we had to make sure that we had full concentration on the task and that no one was in too close proximity of you so that they are in danger of getting injured. We also had be careful not to smash any of the glass equipment as this could cause someone to get cut so to prevent this we tried hard not to smash any of the equipment and any breakages are cleared up following laboratory procedures and practices.
2. Put a rubber-stopper assembly into a 125 mL Erlenmeyer flask. Twist the stopper into the flask's neck until it is tightly secured. Connect the connector and the gas pressure sensor valve, then twist in a clockwise motion. Prevent gas leakage by covering the stopper and flask's top with parafilm. Twist the white valve until it is perpendicular to the valve stem in order to close the two-way valve.
= I have decided to produce a step-by-step guide for each experiment. just to ensure that when we actually come to conducting the practical work, it runs flawlessly. This will also help us conduct fairer tests. as we will be following the same set of steps each time we collect a result.
3. Fill up the 50 milliliter beakers all the way up with deionized water. After, use your scopula to scoop out a small portion of one substance and put in into your beaker. Then put your hot plate onto medium temperature and rest the mixture onto the plate.
6. Place the test tube in the beaker. Secure the test tube and thermometer to the retort stand using clamps. Begin heating the water bath gently.
Second, a small sample of solutions must be collected and transferred to the beakers by pouring or pipetting from the 500 mL Erlenmeyer flasks containing the solution. Third, a clean wooden stick must be dipped into the solution, soaked for three to five seconds, and put to the flame created by the bunsen burner. It is very important to make sure that that the wooden stick should not catch fire and this may have to be done this several times in order to get a good color. Next, the color of the flame must be recorded in detail and the wooden stick must be ran under some running water to cool it off. Finally, the stick must be discarded into the trash and a new wooden stick must be obtained. Dipping the wooden stick into the solution and put toward the flame must be repeated for the remaining solutions and when you are all done all of the solutions , these solutions should be poured down the drain with a lot of water and rinsed with soap and water. Finally, the labels should be cleaned off, and the beakers should be left upside down to
Once reached the chosen temperature place your boiling tubes inside the beaker and leave them to preincubate for 5 minutes
The CDC building is very Hi-tech but there have been issues with structural aspects especially in the labs. Back in 2007 there were lightning strikes in Atlanta that struck the new $214 million BSL-4 facility and both the backup generator and power supply failed to be turned on. The outage caused the containment laboratory’s negative air pressure system to shut down. Negative airflow prevents germs from leaking out of the labs. After investigation, the CDC declared that a critical cable was cut by construction workers that were digging in an adjacent site. Luckily the BSL_4 building was not operational at the time, or else there could have been leaked pathogens (Wright, 2010).
15. Make sure that your long pipette, beaker, dowel and graduated cylinder are all clean.
- Take care when handling glassware eg. Thermometers, beakers, stirring rod and test tubes, to minimize breakage keep glassware away from the edges of the bench and be careful when holding and moving them around.
borate) and 1.0 g. of sodium hydroxide in 20 mL of warm water. It may
solutions and add them to a test tube rack. I will then cut up a
Stepping foot into the lab meant one thing for sure: conducting a new experiment. One time, the instructor distributed petri dishes and gave us instructions to gather specimens of bacteria. I ran excitedly out of the lab and then sat in a corner to gather my sample. The next day we used compound microscopes to examine our samples. Other times, I’d dissect and identify myriad parts of animals like hearts and brains. Cutting through the layers of the cerebrum while smelling the suffocating
used firewood. I can recall putting wood in the heater and taking a poker to stir the