Gram Stain Lab Report

Each student in Microbiology, went through a series of experiments to determine the unknown microbe that was given. The microorganism was inoculated to different test that in 48 hours the solution would either change or remain unchanged. Though some of the experiments required additional steps that was done on the next day of lab. Not only was a series of test done, but also a gram stain was recommended during the discovery of the unknown. The gram stain played a major step, if this step was done correctly students could cross out organisms that did not come near to theirs. By looking through the microscope, the morphology of the bacteria was distinguished by the steps of staining the slide correctly and the oil immersion that clearly shows the shape of the bacteria. The gram stain helped to either categorize your microbe as a gram negative/positive rod or gram positive cocci.

The background of a gram negative will show layers of the cell wall that hold layers that are peptidoglycan and it also shows that this bacteria has an outer layer that is asymmetrical bilayer (Oliver,2017, p. 33-34). The gram negative rod appeared pink with a thin-long stretched out circular shape. This appeared on the slide once the correct magnitude was place and oil was added. From the results of the gram stain, many of the bacteria were crossed of the chart and it only left two microbes. These two organisms which were Escherichia coli and Enterobacter aerogenes made it difficult to choose from because some of the test matched for both organisms. From the start, it was a difficult choice at first. The result seemed that it could have been E.aerogenes, but with studying the results the choice of E.coli fit all the results more than the first