Wait a second!
More handpicked essays just for you.
More handpicked essays just for you.
Practical report of gram staining
Gram Staining Quizlet
Importance of gram staining in microbiology
Don’t take our word for it - see why 10 million students trust us with their essay needs.
Recommended: Practical report of gram staining
In the late 1800s, Hans Christian Gram developed the gram staining procedure. Gram staining is a valuable diagnostic tool used in the clinical and research world. The gram stain is a method used to determine the identification of unknown bacteria. (BIO215, 2017)
According to Healthline.com, typically when you’re sick, you go to the doctors. If your doctor happens to suspect that you may have an infection, he or she may order to have a culture, and/or a gram stain done to check for bacteria. If it happens to be that you do have a bacterial infection, your doctor can then have a gram stain done on the bacteria to see if the bacteria in your infection are gram negative or gram positive bacteria. A gram stain can be performed on various types
One bacterium was gram negative. It underwent four different tests. These tests were the EMB test (Eosin Mehylene Blue), the Sulfur Indole Motility (SIM) test, the Urease test, and the Simmon’s Citrate Utilization test. The EMB test checks for a bacteria’s ability to ferment lactose. This test is accomplished by placing the bacteria on Eosin Methylene Blue agar. The agar is selective for gram negative bacteria and those bacteria that can ferment lactose will have colored growth, usually a metallic green sheen.
After the end of the experiment, the unknown 10 sample was Staphylococcus epidermidis. Come to this conclusion by first beginning with a Gram Stain test. By doing this test it would be easier to determine which route to take on the man-made flow chart. Gram positive and gram negative bacteria have a set of different tests to help determine the unknown bacterium. Based on the different tests that were conducted in lab during the semester, it was determined that the blood agar, MSA, and catalase tests are used for gram positive bacteria while Macconkey, EMB, TSI, and citrate tests are used for gram negative bacteria.
However with septic shock the symptoms may be worse as the patient may experience tachyeordia and tachypnae, leucocytosis which is a high count of white blood cells, change in metal state for example confusion and hyperglycaemia which is a deficiency of glucose in the bloodstream. The diagnosis for sepsis and septic shock involve using blood cultures which is where bacteria is detected in blood which may have spread from a different part of the body. Blood cultures are taken mostly to be done on new-borns and young children who have the symptoms of sepsis. If the blood culture tests positive there is a bacterial or fungal infection which needs to be treated immediately as it is life-threatening. Also cytokines are used as they can destroy the infection however there is a problem with this diagnosis as excessive production can cause tissue and organ
I also inoculated a tryptic soy broth (TSB), a nutrient gelatin deep, a motility agar deep, a fluid thioglycollate medium (FTM) tube, and a TSA plate with my unknown culture. All of these inoculated media were incubated until the next class period (about 48 hours). Then when I came to class most of my inoculated tubes and my streak plate appeared to have growth. The next step I took was making a gram stain to determine the gram reaction and cellular morphology of my unknown. I used my working slant to do this, after careful examination of the gram stain, I learned that my unknown was a gram-positive bacterium. I then preceded by making a negative stain to see the size of the cells of my unknown bacteria. The cell shape was cocci and the cells occurred in clusters of tetrads. After discovering that my unknown bacteria was gram-positive cocci, I turned to page 207 of the lab manual to narrow down my options, there was only four out of the gram-positive list that were
Tests show that your blood or saliva contains a higher than normal level of eosinophils, special cells that fight infection.
Halitosis is the medical term for Bad Breath. When people think of bad breath they automatically think that food is the cause of the bad odor. When in reality there are many factors that can lead up to bad breath. Yes food is one of them but there are other reasons as to why a person may be experiencing bad breath or teeth staining. A person may experience bad breath or teeth staining because they may have a health problem that is causing the odor other factors are smoking and chewing tobacco.
1 / 3 BIO3001 Shinhye Jeon (Heather) Professor Wahlert November 21, 2017 Identifying of Spores Belonging to the Division Pterophyta by Utilizing Phylogenetical method 1. Abstract
I identified the genus and species of an unknown bacterial culture, #16, and I applied the following knowledge of morphologic, cultural and metabolic characteristics of the unknown microorganism according to the laboratory manual as well as my class notes and power point print outs. I was given an incubated agar slant labeled #16 and a rack of different tests to either examine or perform myself; the tests are as follows: Gram Stain; Nutrient Gelatin Test; Carbohydrate Fermentation; Dextrose, Lactose and Sucrose; IMVIC tests; Citrate, Indole, Mythel-Red and Vogues Proskauer test; as well as a Urease and TSI Test.
Diagnosis is crucial because the infection can have similar symptoms to the flu. Examining the skin is one way to identify what type of skin infection is present. Laboratory analysis of fluid and tissue samples are collected to identify the particular bacteria that is causing the infection.
Peschel, O., Kunz, S. N., Rothschild, M. A., & Mützel, E. (2011). Blood stain pattern analysis. Forensic science, medicine, and pathology, 7(3), 257-270. http://link.springer.com/article/10.1007/s12024-010-9198-1
Fischbach, Frances, A Manual of Laboratory & Diagnostic Tests, 4th ed., J. B. Lippincott Company, Philadelphia
Imaging tests. Imaging tests usually aren’t necessary, but in some instances, especially when no evidence of infection is found, They may be helpful. For example, an X-ray or ultrasound may help out other potential causes inflammation, such as a tumor or structure
Biological monitoring is basically evaluating a sterilization process by rendering highly resistant bacterial spores biologically inert. The highly resistant bacterial spores used varies depending on what kind of sterilizer was used. For example Bacillus stearothermophilus spores for steam and chemical vapor sterilizers, Bacillus subtilis spores for dry heat and ethylene oxide sterilizers. These specific Bacillus spores are used because they are more resistant, and present in greater numbers than are the common microbial contaminants found on patient care equipment. If it is proven that these spores have been killed, it is strongly implied that other potential pathogens in the load have also been killed.
use of blood stains. Also in this year the Galton-Henry system was officially used by
stains on sputum’s and body fluids, and have completed a few AFB cultures. Apart from