sterilization

555 Words2 Pages
bulb-icon

Recommended: Conclusion on steam sterilization

The only way to ensure that sterilization has occurred is to use the biological monitoring methods. This is because these are the only tests done which show whether or not actual microbial life has been killed. Biological methods are the only ones which are recommended by the CDC (Centers for Disease Control and Prevention), the AAMI (Association for the Advancement of Medical Instrumentation), the AMA (American Medical Association), OSHA, and OSAP (Office Safety and Asepsis Procedures Foundation)
Biological monitoring is basically evaluating a sterilization process by rendering highly resistant bacterial spores biologically inert. The highly resistant bacterial spores used varies depending on what kind of sterilizer was used. For example Bacillus stearothermophilus spores for steam and chemical vapor sterilizers, Bacillus subtilis spores for dry heat and ethylene oxide sterilizers. These specific Bacillus spores are used because they are more resistant, and present in greater numbers than are the common microbial contaminants found on patient care equipment. If it is proven that these spores have been killed, it is strongly implied that other potential pathogens in the load have also been killed.
In this method, living spores which are resistant to whichever sterilizing agent is being tested are prepared in either a self contained system, such as dry sp...

Show More
Related

  • The Affect of Environmental Conditions on Gene to Map Distance in Sordaria Fimicola

    948 Words  | 2 Pages

    Each student had to count, at least 100 spores, in order to determine if crossing over occurred in a particular perithecium. Data was collected to determine whether various environmental conditions affected the crossing over in Sordaria sp. In the case of temperatures, the cultures were incubated at each determined temperature. For the UV radiation, cells were exposed to UV light for 10 seconds and then grown at 30oC. For the EtBr treatment, 50ul of EtBr was added to the growth medium and cells were incubated at 30oC. In the case of sunlight exposure, cells were exposed to sunlight directly and grown at room temperature.

    Read More

  • Identifying Two Unknown Species of Bacteria

    1333 Words  | 3 Pages

    After 48 hours of incubation the agar plates were viewed. Individual colonies were tested for successful isolation by gram staining and then viewing the stained bacteria under a microscope. Isolation was successful. One colony of each unknown bacteria was transferred to an agar slant for growth. The agar slants were stored at room temperature over the weekend so that they would not grow too much.

    Read More

  • Identifying An Unknown Bacterium From A Mixed Culture, By Conducting Different Biochemical Tests

    1612 Words  | 4 Pages

    They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species.

    Read More

  • Factors That May Cause Contamination During Filtration

    1621 Words  | 4 Pages

    Comment on class result with respect to differences in filter types, differences in filter assemblies, and overall on the confidence you would have in using this type of sterilisation process in preparation of pharmaceutical products. List the factors that may cause contamination during filtration. (20 marks)

    Read More

  • S. P. D Technician Essay

    749 Words  | 2 Pages

    STERILIZATION Competent SPD (sterile processing department) technicians must be knowledgeable about all available sterilization methods, regardless of whether those methods are currently being used at their facility. The sterilization equipment, sterilization parameters, and loading and unloading of sterilizers are all critical components of effective sterilization .in addition, sterilization processes must be monitored administratively, biologically, and chemically. It is important that all

    Read More

  • Sterile Compounding Essay

    1221 Words  | 3 Pages

    There are three risk levels (low, medium and high) associated with sterile compounding. CSPs are assigned a risk level based on the probability of contaminating the preparation during compounding with microbial contaminants e.g. spores or endotoxins and chemical and physical contaminants e.g. skin particles from staff or broken glass. Once a CSP is classified, a licensed healthcare professional overseeing the sterile compounding can then decide upon the most suitable procedure and environmental quality of the facility which are necessary for preparing the CSP. The risk levels apply to the quality of the CSP d...

    Read More

  • Prion Disease Essay

    2380 Words  | 5 Pages

    Following the proper guidelines for reducing the risk of transmission of TSEs in a clinical setting, the risk has been greatly reduced. As mentioned before no iatrogenic transmission from a medical instrument has been recorded since 1976 since following the standard guidelines. In an experiment by Amin et al., they examined the risk of transmission of vCJD via contact tonometry. They outlined that transmission could be further reduced by using larger volumes of washes for extended periods of time and also adding wiping between washes (16). This could help in the future to completely eradicate TSE transmission completely by increasing sterilisation methods. Proper training on all aspects of transmission of TSEs through medical equipment and proper sterilisation methods should be updated just to keep staff up to date to prevent the risks of TSEs transmission.

    Read More

  • Symptoms and Modes of Infection of Bacillus Anthracis

    1766 Words  | 4 Pages

    Bacillus anthracis (anthrax) is a bacterium that infects organisms through sporulation. Bacillus anthracis is can be aerobic (needs oxygen) or, facultative anaerobic (does not require oxygen). It is also a gram positive bacterium that can be found in the soil of humid and damp climates (Hicks, 2012). The bacterium is part of the species Bacillus Cereus (Koehler, 2009). It is known to infect life forms four different ways. Infection can be caused through digestion, eating food that is contaminated with the bacteria. Another way is through inhaling; which occurs through breathing in the spores. The third way of becoming infected is through the skin, known as cutaneous; this infection occurs through open wounds. The newest form discovered of infection is through injection; the use of needles (Hicks, 2012). Though it is not common to be found in wealthy countries; there have been numerous outbreaks in Europe and the United States. It is also being considered for bioterrorism (Sweeney, 2011). Symptoms of the early stages of anthrax can be misunderstood for less lethal conditions (Hicks, 2012). For patients with a possible anthrax infection can be determined by gram positive staining. It can also be diagnosed by confirmatory testing, such as PCR (Polymerase Chain Reaction) (Sweeney, 2011). PCR focuses on certain DNA strands for replication and identifying what the organism is (Genetic, 2014). Anthrax toxin antagonists can be considered for healing; but antibiotic therapy is the most common use of treatment (Sweeney, 2011).

    Read More

  • Body Hygiene Case Study

    1258 Words  | 3 Pages

    The extern washing her hands before setting up the examination room is important because she will be touching sterilized equipment, and in order to prevent cross contamination, gloves and hand hygiene is not only important but necessary.

    Read More

  • Voluntary Abortion or Compulsory Sterilization?

    1948 Words  | 4 Pages

    Starting in the mid-1960s, some erosion of the anti-abortion laws began to take place. But these efforts have not been supported by many of the more vocal groups who are trying to do something about excess population growth; to them, compulsory birth control and compulsory sterilization are apparently more palatable than voluntary abortion.

    Read More

  • Fertility Treatments

    3796 Words  | 8 Pages

    Malpani, Dr. A&A. (2004). How Much Does Treatment Cost?. Retrieved November 1, 2004, from the World Wide Web: http://infertility.adoption.com/chapter47.php

    Read More

  • Spaying and Neutering

    1496 Words  | 3 Pages

    Many people don’t spay or neuter their pets, especially cats. There are a lot of benefits and yet according to The Humane Society of the United States, six to eight million cats enter the shelters each year and while three to four million are adopted out, another three to four million are killed. “An unsprayed female cats, her mate and all of their offspring producing just 2 litters per year (the average is 3-4), with just 2.8 surviving kittens per litter can total to 66,088 cats in just 6 years” (Goldstein & O’Keefe 4).

    Read More

  • Essay On Infection Control

    1123 Words  | 3 Pages

    ...s and hoses, control buttons, switches, hand pieces, and X-ray units (Collins). After every patient’s visit, the operatory is to be sterilized and disinfected. All areas that were that were not covered with a barrier, or if the barrier was compromised, must be wiped down with surface disinfectants similarly used in hospitals. OSHA requires disinfectants to be potent enough to fight against HIV and HBV infections (Collins). If there is any blood present on a surface, tuberculocidal type disinfectant should be utilized.

    Read More

  • Medicaid Sterilization

    1102 Words  | 3 Pages

    Is sterilization for those on Medicaid, correct? Or is it unethical? This is a topic that for many years has been controversial. History of sterilization for women without their consent goes way back when U.S. women of color were victims of forced sterilization. Some women were sterilized during C-sections and they were never told. Other women were threatened with termination of welfare benefits or denial of medical care if they didn’t consent to the procedure (Garcia, 2013). In the late 1970s, in an effort to protect women’s reproductive rights, federal legislation preventing sterilization of women without their consent was passed. One of the most important features of this legislation is that it applies

    Read More

  • Abortion Procedure

    684 Words  | 2 Pages

    Procedure of Abortion has been known since ancient times as a crude method of birth control. Many Abortions were practiced. Abortion was not illegal in most countries until the 19th century. Abortion is when a pregnancy is ended so that it does not result in the birth of a child. Sometimes it is called “termination of pregnancy” British Pregnancy Advisory Service (BPAS) cares for women with an unplanned or unwanted pregnancy, They help women who have decided that abortion is the right choice for them and give them advice and counselling to women who do not know what to do next. Abortion is defined in the following ways, all of which stop pregnancy, miscarriage, surgical abortion, and injection of medicine.

    Read More

More about sterilization

Open Document