Unknown Bacteria Identification Lab Experiment

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Unknown Bacteria Identification Lab Report
Introduction
The unknown identification laboratory test is made for students to guide themselves into the microbiology laboratory. Students will use the producers, techniques and experiment that they have been used and learned in other pervious classes. During previous laboratory test, many bacteria have been tested and experimented in finding the results of what bacteria was truly growing. There are so many bacteria in our surroundings many of them can be found all around us and in our environment. Some of the bacteria have been tested and are known to help patients when they are sick, they can treat diseases, certain foods, and make antibiotics and many other things. Thought-out this laboratory …show more content…

Each group of bacteria was labeled with a different number. The objective was to find what three bacteria’s. Each student had obtained by doing different types of experiments. The first step was to streak the three methods in an aseptic technique. The two gram negative bacteria’s were to be T streak with one Helton Enteric, one streak of Mac Conkey, and one streak with Eosin methylene blue agar also known as EMB. The procedure was done just as it in the laboratory manual indicated. All the test tubes and plates were label and placed into the incubate to test for isolation for the next 48 hours. However, for the gram positive bacterium there was a streak with one Phenyl enthol Blood agar and also placed in the incubator for 48 …show more content…

After the observation of the agar plate of EMB had one the best isolated colonies with a pink moist isolated colorful and clear colonies. Mac Conkey was pink moist also had isolated colonies however, many of them were too close together and could cross contaminated the bacteria. The Heckton enteric was green black also had many colonies but the colonies were not isolated as much as the other plates. After a close observation of the EMB it was more possible and useful to test the plate for isolation. The next biochemical test for Entries required being examed by the medium of the Triple sugar Iron Agar test as known as the TSI. The tube would streak and stab at the center and will be incubated for the next 48 hours. The gram positive was observed with the PE Blood agar plate. The plate had a creamy white growth on the red blood agar. First the there was a smear on a slide, it was observed under the microscopy it was determine to find the cellular morphology and gram reaction. The gram positive bacterium was rob and a positive purple magenta. However the gram positive bacilli were streak with a starch plate and incubate for the next 48

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