In the Transformation lab, we saw how protein glowed in bacteria. First we scooped out bacteria from a petri dish and added it into positive and negative labeled test tubes. We then pipetted calcium chloride into the test tubes. The calcium chloride adds positive charges into the mix. The test tubes are then placed into an ice bath to cool the bacteria down before they were removed and put into hot water. This process kills some of the bacteria creating an apocalyptic environment. Those that did not die took in an extra plasmid in an attempt to survive. The plasmid helped them survive the heat. Not all bacteria took in plasmid. The tubes are then placed back into ice to calm the bacteria. The bacteria was then pipetted out of the test tubes …show more content…
All the bacteria on the petri dish took in the plasmid and are therefore alive. The gene did not glow because Arabinose was not present and they could not turn on the operon. LB/ Amp /Ara with pglo glowed because the arabinose turned on the operon that controlled glowing. The bacteria is now making arabinose, causing them to glow. Also, all the bacteria on the petri dish were resistant to Ampicillin. Some of the bacteria colonies that did not glow had metabolized, or used up, all the arabinose. Bacterial transformation has shown great promise and progress in medicine and agriculture. It helps with research of using Insulin to treat diabetics, creating, and inserting cell hosts. Botanists use bacterial transformation to experiment with plants to resist colder temperatures, playing with the ripening process, and their ability to form a resistance to pests. However, these are always contradicted with statements challenging the ethical and moral issues that have to be unravelled with much thought before the further use of bacterial transformation. This process has opened doors for a healthier future and new, easier ways to create life saving
Once the recombinant plasmid was obtained, it was then inserted into E. coli cells through transformation. From a successful transformation, we expected the bacterial cells to translate the inserted EGFP sequence into its protein form. The bacteria cultures were plated on petri dishes containing growth supplement, Luria Broth (LB), an antibiotic: Kanamycin, and IPTG which induced the fluorescence property within successfully transformed bacterial colonies. Different variants of the petri dishes were also included as control and unknown.
...et light. If the LAA plate glows green under exposure to ultraviolet light, then we can conclude that our unknown insert piece of DNA would be the kan gene. If it does not glow green under exposure to ultraviolet light, then then we streak the colony from our LAA plate onto the LAC plate using a sterile glass spreader. When the LAC plate is dray, we place it upside down in the microfuge rack so that it can be incubated at 37 ºC. Incubation at 37 ºC will allow the transformed bacterial cells to grow. If we see bacterial growth on the LA plate containing chloramphenicol, we can conclude that our unknown insert piece of DNA would be the cat gene, since the cat gene is resistant to chloramphenicol. Afterwards, we then grab the microfuge tube labeled NP and repeat the aforementioned steps shown above pertaining to the LA plates. This would be considered our control.
Therefore colonies containing the non-recombinant pUC19 plasmid have a functional lacz’ gene appear blue on the agar and colonies containing recombinant pUC19 would have a non-functional lacz’ gene due to insertional inactivation and appear white on the growing medium.
The Papanicolaou test in medical terms can also be known as or be called as the Pap smear, pap test, or smear test. It is a microscopic examination of cell .The purpose of this test is to perceive occurring of precancerous or existing cancerous cells in the opening uterus of the cervix. It also discerns asymmetry in the cells of the cervix (the bottom, narrow end of the uterus.)[1]
What data might be collected by the evaluating occupational therapist if he or she is guided by the PEOP Model and why?
1. (45 pts) For each major (CS, IT, DET, IS), identify three important and uniquely identifying characteristics and describe how the four types of professionals would work together in a professional setting. Include information learned from the various instructors for each discipline. How do professionals from these areas work together?
Change 4 Life is a campaign that is set up to promote health and it also helps a range of individuals change their lifestyles by:
“But how did it come to this?” you’re probably asking yourself. Humans may have been studying antibiotics, but so were bacteria – and they’ve b...
As young adolescents make the transition from middle to high school, ambiguous behaviors are becoming increasingly evident. Hoping to improve these behavioral issues among freshmen, certain schools are reestablishing their system so that 9th graders are isolated from the larger high school community. This will result in negative outcomes for the community as well as for these young adults.
He boiled pond water to kill all the microbial growths. He then poured that water into two separate test tubes. He sealed one so that no air could get in, and left one open to the air. The one that was left open slowly became more and more cloudy with microbial growths. The sealed tube stayed as clear as it had been when it was boiled.
As a child, going to school felt like a necessary part in my day, mainly based on the ideas my parents raised me on, but on special days in which we could finally leave the classroom is when I felt most excited to learn. As years passed I entered middle school and eventually high school and my excitement felt as if it disappeared form the routine. I felt it was just my childhood escaping me but I realized why I wasn’t happy with classes. In elementary I would have designated days to do something outside of the class room at least three times a month so it always felt sort of relevant. In middle school I would have two classes which didn’t feel like a boring classroom and that would be physical education and woodshop, while in high school I would always feel trapped in a classroom with a bland glare from the teacher
I am the kind of person who likes to be in an environment that doesn’t change to often. I’m not a fan of jumping into something new. Adapting to change is one of the hardest things for me and it is the one thing that I need to work on the most. Change happens all around us every day and I need to learn how to deal with it. Change can be good. Change can be bad. It’s just what I’m going to have to work on to become a better leader.
The Great Transformation, although not a recently published piece, still presents many values and truths that still hold water today. Polanyi’s piece has held up very well over time, giving the reader a very profound understanding of the issues facing global societies at the dawn of the 21st century. I believe the author is trying to describe to the readers his view on the importance of self-regulating markets and the role they played on global economies (Polanyi pg. 3). Polanyi further adds to that statement by providing his thesis to this paper on pages 3-4, Polanyi states that:
The idea of change is the most constant factor in business today and organisational change therefore plays a crucial role in this highly dynamic environment. It is defined as a company that is going through a transformation and is in a progressive step towards improving their existing capabilities. Organisational change is important as managers need to continue to commit and deliver today but must also think of changes that lie ahead tomorrow. This is a difficult task because management systems are design, and people are rewarded for stability. These two main factors will be discussed with reasons as to why organisational change is necessary for survival, but on the other hand why it is difficult to accomplish.
The transformation of man has long since been a topic of much expanse. Today, the defining of transformation still lingers. Socialization forms the core to many transformations in a student’s life. Since John Lock posited the socialization of man in the Enlightenment period, transformation has become a direct effect often due to life altering experiences – the “liberation of the individual within a dominant, oppressive, technocratic society” (Module 4 Theory and Analysis). Paulo Freire says transformation is essential to relationship with others.