Petri Dish Lab Report Sample

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Sample collection and Growth:
Sterile cotton swab was used to collect a sample from two objects, as well as leaving out a petri dish to open air. One object was unwashed hands and the other object was the back of a cell phone. The two samples were transferred to two separate sterile petri dishes, these petri dishes where all labeled with initials, lab section, and place collected from. All three petri dishes where given to TA who proceeded to place them in an incubator that was at 37° C and allowed to grow to form colonies.
Examination and Selection of colonies:
By using aseptic technique, which consisted of using gloves and wiping area with disinfectant, we were then able to inspect the growth on the three dishes, without removing the lids. We compared what we saw to samples we looked at and took notes on the week prior. A small live culture of the unknown bacteria was then made from a petri dish of our choosing. The colony had to be isolated and fairly large. In a 1.5 ml micro centrifuge tube 400 µl of sterile water were mixed with about half of our selected colony, which was transferred by using the broad end of a sterile toothpick and taking from our petri dish.
PCR Amplification & Purification of 16s rRNA gene:
In a new …show more content…

For the catalase test a clean microscope slide was placed in a petri dish, fresh bacterial cells were smeared on the center of the slide, then a drop of 3% hydrogen peroxide was administered onto the bacterial cells, and formation of bubbles was observed. MSA test was conducted by obtaining a petri dish containing MSA medium and .5 ml tube. A sample of the bacteria along with 200 µl of sterile water are mixed in the tube, 5-10 sterile beads and 50 µl of the newly made bacterial solution are added to the MSA petri dish and the solution is spread out using the beads. The petri dish is then incubated for 2 days at 37°C, and evaluated for

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