coliform as well as Escherichia coli. Humans as well as animals come into contact with these areas, some are used for recreational activities such as swimming and some are a source of drinking water for both animals and humans The main goal of this experiment was to see which lakes, snow run off and ponds tested positive for coliform or Escherichia coli and to come up with some reasoning as to why. It was found that the more remote pond with less contact contained the most Escherichia coli. However, another
Escherichia coli (E. coli) is a member of the Enterobacteriaceae family of organisms. It is a non-spore forming, facultative anaerobic, gram negative rod capable of growing on a variety of media and, similar to other members of the Enterobacteriaceae family, contains the enterobacterial common antigen. Most E. coli are part of the normal flora of the human gastrointestinal tract, however some strains are pathogenic and capable of causing clinical disease. Epidemiologic classification of E. coli is
Escherichia coli are a bacterium that is a part of the Enterobacteriaceae family and it is in the Gamma Proteobacteria class. Escherichia coli are gram negative and have a straight rod shape. According to the Centers for Disease Control and Prevention, Escherichia coli are important for the health of the human intestinal tract (Escherichia coli, 2013)2. Even though Escherichia coli are important for the intestines it can also cause illness. Escherichia coli (2013)2 states there are certain
Cystitis effects both genders, the young, the old and can lead to complications that can do irreparable damage to the urinary tract. The purpose of this paper is to discuss the disease of cystitis and relate the specific virulence factors of Escherichia coli to the prevalence and symptoms of cystitis. This will be accomplished by defining the disease, its etiology and the causative agent. The mode of transmission and risk factors will be discussed as well, the pathogenesis, signs and symptoms will
Escherichia coli is a member of the family Enterobacteriaceae. It is a bacterium with a cell wall that has many components. Escherichia coli can live without oxygen which means that it is a facultative anaerobe. It is also capable of fermenting lactose under anaerobic conditions, and in the absence of alternative electron acceptors. There are effects and various factors that limit its growth rate. Its morphology consists of a rod-shaped gram negative bacteria that is commonly found in soil, water
Modified Ames Test for Mutagenicity of Pepsi-Cola in Escherichia coli I. Introduction Certain substances have been avoided because of their potential harmful effects on the body. One way a substance has affected cells was by changing their genetic material. A substances’ potential to cause changes in genetic information was the mutagenicity of the substance. If it caused changes in the DNA sequence, it was considered a mutagen. The altering of a DNA sequence was a concern because the modification
Gentamycin, Neomycin, and Streptomycin on the Escherichia coli Bacteria Introduction: Escherichia coli, commonly known as E. coli, is a bacterium that is associated with food poisoning. Both in the medical community and the general public there are growing concerns about the health dangers that are associated with Escherichia coli. One major area of concern is its apparent resistance to certain core antibiotics. The bacterium Escherichia coli, is found in both foods and lakes. In terms of food
microscope. This indicated that the unknown bacterium was gram-negative. Microscopic examination of the unknown bacterium also indicated that the bacterium was bacillus(rod) shaped. The positive control used for microscopic examination was Escherichi coli and the negative control was Staphylococcus aureus. Catalase The Catalase Test indicated a positive result. This showed that the bacterium contained the enzyme catalase which converts hydrogen peroxide into water and gaseous oxygen. The
acquired either as health care associated or in the community. The cause of such infection also includes the following but not limited to poor hygiene, sex, instrumentation, anatomic structure, etc. [6]and Out of the several causative agents, Escherichia coli and other coliforms played as major causative agents[7,10]. Urinary tract Infections are commonly treated with sulfamethoxazole - trimethoprim and fluoroquinolones[7,10,12]. However, due to the frequency of antibiotic use, recurrent or chronic
stomach for several days after the initial onset of the illness. In severe cases of food poisoning, patients can see blood in their vomit or diarrhea. In extreme cases of Escherichia coli food poisoning, kidney failure can be experienced. There can be death from food poisoning, but it is rare (Lowinger 1). Like Escherichia coli, Listeria and Campylobacter can have some very extreme cases that can potentially cause major
different I couldn’t get the result of the TSIA as A/A but instead K/A. I look back to the microbiology slide on lab 5 and compare my result to the picture on the slide to help me figure it out. Therefore it was concluded that the unknown was Escherichia coli.
bacterium has the enzyme tryptophanase, indole will be ... ... middle of paper ... ...indole, it is motile, there is no urease present and there is no coagulase activity. By deduction and logical reasoning Unknown 10a was determined to be Escherichia coli. Unknown 10b is Staphylococcus epidermidis. According to Bergey’s Manual Staphylococcus bacteria are gram positive spherical cells that occur singly, in pairs or in irregular clusters. Unknown 10b was gram positive, spherical and occurred
of our isolate A bacteria may be due to a mutation that affects the outer membrane of the gram-negative bacteria; consequently, the antibiotic is able to penetrate the bacteria and bind to the ribosome. Mutant strains of the enteric bacteria Escherichia coli and Salmonella typhimurium that are unable to synthesize lipid A and lipopolysaccharide, components of the outer membrane, were found to be more susceptible to Azithromycin since the antibiotic was able to diffuse through the outer membrane (Vaara
Introduction: Topic: Micro-organisms Question: Does cinnamon and curry inhibit the growth of bacteria? Hypothesis: Cinnamon and curry do inhibit the growth of bacteria. Cinnamon inhibit growth better than curry does. Aim: • To see if cinnamon and curry are potent agents in fighting diseases • To compare the ability to inhibit bacterial growth between curry and cinnamon How: I plan to use agar plates in my experiment. There will be a group of 3 petri-dishes: one with just bacteria, another
middle of paper ... ... 1st seven amino acids of b-galactosidase to fuse with that of growth hormone inhibitory hormone i.e. Somatostatin. Somatostatin was not detected in transformed bacteria the possible explanation was that it degraded in E. coli bacteria. So the other plasmid was genetically formed to prevent the degradation which had mutually a promoter of the lac operon and another lacL region. The gene for beta-galactosidase is lacZ. This time plasmid was sliced close to c-terminus of beta-galactosidase
Coli. The most prominent growth was seen on the plate with Luria-Bertoni (LB) broth and ampicillin (amp) treated with +pGLO, as there were four separate and defined bacterial colonies with surrounding satellite colonies, each of which contained upwards
Lipps 2003, Lipps 2008 B). Our solution is to create a universal antivenom is modify a strain of Escherichia coli to produce LT-15 (Lipps & Lipps 2005). We will insert the LT-15 gene into a plasmid with a promoter, then transform E. coli with the plasmid and grow the recombinant strain. (Cawood 2013, Cohen et al. 1973, Huang et al. 2012, Lipps 2002 B, Lodish et al. 2000, Muyrersa et al. 2001). These E. coli can then be grown and harvested industrially for LT-15. This is a novel project because this would
known bacteria that stained opposite of unknown. We were given the size of the known bacteria and performed a comparative analysis under the microscope to determine the size of our unknown. In my case the control was a gram-negative bacteria Escherichia coli. Acid-fast Stain: The Acid-fast stain is used to determine whether are unknown contains a high amount of mycolic acid in its cell wall. Those organisms that are acid fast will retain the basic fuschin stain while those who are not will have this
We observed the agar plate for colonies that had a metallic look, because these were likely E.coli colonies. We then recorded what we saw. After selecting what we believed to be an E. coli coliform colony, we inoculated a TSA slant, in addition to another lactose broth, and incubated these for another 24 hours at 37°C. After incubation, we checked the broth for the production gas and a color change from red to yellow which would indicate
experiment was to verify induced transformation in naturally incompetent E. coli HB101. The pGLO plasmid was the means of transformation in this experiment. The pGLO plasmid contains three core genes: the bla gene encoding for ampicillin resistance, the gfp gene encoding for the green fluorescent protein, and the araC gene which activates the gfp gene in the presence of arabinose. The CaCl2 – heat shock method was used to transform E. coli HB101. After plating the bacteria, the petri dishes containing LB as