Ariel Nadler
The Effectiveness of the Antibiotics, Gentamycin, Neomycin, and Streptomycin on the Escherichia coli Bacteria
Introduction:
Escherichia coli, commonly known as E. coli, is a bacterium that is associated with food poisoning. Both in the medical community and the general public there are growing concerns about the health dangers that are associated with Escherichia coli. One major area of concern is its apparent resistance to certain core antibiotics. The bacterium Escherichia coli, is found in both foods and lakes. In terms of food it is mostly beef and dairy products, and sometimes in vegetables due to the fertilizer being partially cow feces. In terms of lakes, E coli can be in any of them. This bacterium causes humans to
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The slide was placed on a staining tray where the sample was stained using crystal violet. After a minute the sample was rinsed. After that Gram iodine was put on the sample and was rinsed again, after a minute. Following that a 95% alcohol/acetone was dropped on the sample until only a faint violet like color is seen. Immediately following that the slide was rinsed in order to prevent further destaining. Then the sample was covered with safranin for 45 seconds in order to restain the destained gram-negative bacteria making it have a purple-pinkish color. Following this the slide was rinsed and the sample was observed.
Results: The bacterial lawn of Escherichia coli consisted of the antibiotic saturated disks, very few white dot like structures, which are probably colonies of the bacteria, and the zones of inhibition of each of the antibiotics as shown in Figure 1. Each zone of inhibition had a different diameter showing which antibiotic is more effective. Figure 1. The zones of inhibition produced by the antibiotic disks in E. coli bacterial lawn. (Top right: Gentamycin, Top left:
Streptomycin, Bottom left: Neomycin, Bottom right:
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After ten minutes had passed, I collected the ingredients needed to perform a gram stain. I got the primary stain, crystal violet, and flooded my smear for sixty seconds, and then rinsed the color off with water until the water ran clear. I then flooded the smear with the mordant, grams of iodine, and let that sit on the slide for sixty seconds as well. I then rinsed the grams of iodine off with water and applied alcohol to the smear to decolorize the cells; however I made sure not to over decolorize and only put enough drops on the smear till the purple ran clear. I then rinsed the slide with water and flooded the smear with safranin, the counter stain and let it sit for sixty seconds and then rinsed the color off with water.
Streak plate technique was used to isolate pure culture for each bacteria (2). The Gram stain was used to determine Gram reaction and morphology of each bacteria (2) Selective and differential media such as, salt agar, MacConkey agar and blood agar were used for bacterial identification (2). Gelatin deeps were inoculated to detect production of gelatinase (2). Starch Agar plate were inoculated to detect amylase (2). Ocular reticle used to determine bacteria size (2). Motility deeps were inoculated to detect motility on bacteria (2). Thioglycollate broth used to determine oxygen requirements (2). Carbohydrate fermentation
I also inoculated a tryptic soy broth (TSB), a nutrient gelatin deep, a motility agar deep, a fluid thioglycollate medium (FTM) tube, and a TSA plate with my unknown culture. All of these inoculated media were incubated until the next class period (about 48 hours). Then when I came to class most of my inoculated tubes and my streak plate appeared to have growth. The next step I took was making a gram stain to determine the gram reaction and cellular morphology of my unknown. I used my working slant to do this, after careful examination of the gram stain, I learned that my unknown was a gram-positive bacterium. I then preceded by making a negative stain to see the size of the cells of my unknown bacteria. The cell shape was cocci and the cells occurred in clusters of tetrads. After discovering that my unknown bacteria was gram-positive cocci, I turned to page 207 of the lab manual to narrow down my options, there was only four out of the gram-positive list that were
Bacterial resistance to antibiotics has presented many problems in our society, including an increased chance of fatality due to infections that could have otherwise been treated with success. Antibiotics are used to treat bacterial infections, but overexposure to these drugs give the bacteria more opportunities to mutate, forming resistant strains. Through natural selection, those few mutated bacteria are able to survive treatments of antibiotics and then pass on their genes to other bacterial cells through lateral gene transfer (Zhaxybayeva, 2011). Once resistance builds in one patient, it is possible for the strain to be transmitted to others through improper hygiene and failure to isolate patients in hospitals.
Pseudomonas aeruginosa (P. aeruginosa) is a gram-negative, rod-shaped aerobic bacterium. It is a primary cause of hospital-acquired infections. P. aeruginosa is primarily a nosocomial pathogen. It also acts as an opportunistic pathogen, which can only infect a host that is immunocompromised, due to an underlying disease or medication. Although, P. aeruginosa can cause damage to virtually any tissue in the body, it almost never affects the tissues of healthy individuals. It is a problematic pathogen in hospitals; infecting individuals with cancer, burn wound, catheters and cystic fibrosis. P. aeruginosa is most recognized for its resistance to a wide range of antibiotics. In its planktonic form, P. aeruginosa has been found to have many virulence factors. However, P. aeruginosa within biofilms have been found to have a resistance to antibiotics 1,000 times greater than that of its planktonic counterparts [4]. Infections that are caused by bacterial biofilms are very persistent and very difficult to treat.
Among hospitalized patients around the world, Clostridium difficile is the primary source of infectious diarrhea. Previously, continuously unbalanced intestinal microbiota, usually due to antimicrobials, was deemed a precondition of developing the infection. However, recently, there have been alterations in the biology from virtually infecting the elderly population exclusively, wherein the microbiota in their guts have been interrupted by antimicrobials, to currently infecting individuals within of all age groups displaying no recent antimicrobial use. Furthermore, recent reports have confirmed critical occurrences among groups previously assumed to be of minimal risk—pregnant women, children, and individuals with no previous exposure to antimicrobials, for instance. Unfortunately, this Gram-positive, toxin-producing anaerobic bacterium is estimated to cost US critical care facilities $800 million per year at present, suggesting the need for effective measures to eliminate this nosocomial infection (Yakob, Riley, Paterson, & Clements, 2013).
Another campaign developed by the CDC is the Transatlantic Taskforce on Antimicrobial Resistance (TATFAR). This task force focused on urgent antimicrobial resistance issues and appropriate therapeutic use of antimicrobial drugs in the medical and veterinary communities. Also, prevention of both healthcare- and community-associated drug-resistant infections, and strategies for improving the pipeline of new antimicrobial drugs()
Household bacteria have always been a problem. Millions of people each year get some sort of sickness from bacteria in their kitchen, bathroom, living room, etc. What if there were bacteria in your kitchen that could be deadly? This bacterium is called Escherichia coli. According to Oregon health Authority: “By one estimate, 10,000 to 20,000 E coli. Infections occur in the United States each year.” Many of us use disinfectants such as Clorox and Bleach every day but are these disinfe...
E. Coli is the bacteria that live in the intestines of animals. Most types of these bacteria are harmless provided that it is not very high levels. There are some types that can make you sick.
Bacteria that is resistant to antibiotics is a major problem not only for the United States, but worldwide. According to the Centers for Disease Control and Prevention (2012) the cause is related to “widespread overuse, as well as inappropriate use, of antibiotics that is fueling antibiotic resistance”. According to World Health Organization (2013) resistance is a global concern for several reasons; it impedes the control of infectious diseases, increases healthcare costs, and the death rate for patients with resistant bacterial infections is twice of those with non-resistant bacterial infections.
Antibiotic resistance evolves naturally through natural selection via random mutation, but it could also be engineered by applying an evolutionary stress on a population. The antibiotic action is an environmental pressure; those bacteria which have a mutation allowing them to survive will live on to reproduce. They will then pass this trait to their offspring, which will be a fully resistant generation. Studies at the Finnish Academy found that using one type of antibiotic increases the resistance of bacteria to other types of antibiotics as well. Antibiotics do not work against illnesses t...
Every year, antibiotic-resistant bacteria are threatening more and more people. As much of a problem as it is, many people are not educated on the term drug resistance. Since it is such a growing concern, it becomes confusing as to why drug resistance is occurring and what can be done to prevent it. Because drug resistance is such a health problem, determining what it is, how these bacteria can acquire the antimicrobial agents, and the possible solutions to the resistance are the types of actions that need to be taken in order to have a better understanding of how truly powerful these drug resistant bacteria are.
Resistance develops as a result of natural selection. Due to continuous exposure, the bacterial organism changes its genetic make up and these bacteria with mutations will pass the trait that helps then survive antibacterial mechanisms to their offspring which will eventually become a fully resistant generation. Antibiotic r...
Escherichia coli, also referred to as E.coli, is a rod-shaped, gram-negative bacterium. It is about 1-3 micrometres long, and around 0.25 micrometres in diameter (Microbe Wiki. 2004). The bacterium is typically found within the lower intestine of the digestive tracts of warm-blooded organisms, including those of a human. (Zhang Lab. N/A)
Knowledge of basic terms relating to antibiotic resistance is essential for a full understanding of the issue. A brief summary of bacteria, antibiotics, antibiotic resistance is incorporated below, as well as the history of antibiotics. Bacteria are small microorganisms that live everywhere on earth, with the exception of Arctic areas. Bacteria play a vital role in assisting with food digestion, nitrification, and decomposing dead organisms. Pathogens are bacteria that cause disease. Antibiotics are drugs that use microorganisms such as fungi and other bacteria to...