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Basic concepts in microbiology
Basic microbiology
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UNKNOWN PROJECT LAB REPORT
Christian Ramos
Unknown-D8
Due: April 21st
Prof. Berte
MCB2010L
Spring 2016
Introduction
The term “microbiology” refers to the branch of study that deals with microorganisms. Microbiology is extremely important in today’s time for the crucial information that the study provides. Human’s have had a long and cruel history of disease and sickness, for example the bubonic plague, but microbiology gives scientists the ability to observe, study, and prevent sickness like the bubonic plague to ever happen again. At the center of microbiology lies the bacterial cell, one that differs from those of a plant or animal because it lacks a nucleus and membrane-bound organelles which, in turn are traded for pili, flagella, and in some cases a cell capsule. Bacteria that are capable of causing illness or disease are called pathogens, pathogens work by releasing toxins in the body or directly damaging the host’s cells. An article by Lise Wilkinson explains that the earliest categorizations of bacterial cells first occurred in the late eighteen-hundreds to the early nineteen-hundreds by scientists (at the time) O. Muller and C. Ehrenburg (Wilkinson, 2004). The observation and identification of unknown bacteria that emerge is crucial because these new bacteria might be pathogenic and cause illness so it is very important that the bacteria is identified as soon as possible in order to either prevent the upcoming illness or treat it. While the common person is unable to identify if they are carrying bacteria (which is very likely), specialized tests that are ran in a lab can identify different types of bacteria and can even help
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(2004). Foreword. The Lancet, 364(94), 23rd ser., 1-1835. Doi: 10.1016/s0140-6736(04)17616-2
Leboffe, M. J., & Pierce, B. E. (2010). Microbiology: Laboratory Theory and Application, Third Edition 3rd Edition (3rd Ed.). Morton Publishing
The first day an unknown sample was assigned to each group of students. The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The morphology of the two types of bacteria was viewed under the microscope and recorded. Then the sample was put on agar plates using the quadrant streak method for isolation. There were three agar plates; one was incubated at room temperature, the second at 30 degrees Celsius, and the third at 37 degrees Celsius. By placing each plate at a different temperature optimal growth temperature can be predicted for both species of bacteria.
In this lab project, the microbiology students were given 2 unknown bacteria in a mixed broth each broth being numbered. The goal of this project is to determine the species of bacteria in the broth. They had to separate and isolate the bacteria from the mixed broth and ran numerous tests to identify the unknown bacteria. The significance of identifying an unknown bacteria is in a clinical setting. Determining the exact bacteria in order to prescribe the right treatment for the patient. This project is significant for a microbiology students because it gives necessary skills to them for future careers relating to clinical and research work.
They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species.
Bacteria play a large role in our health, the environment, and most aspects of life. They can be used in beneficial ways, such as decomposing wastes, enhancing fertilizer for crops, and breaking down of substances that our bodies cannot. However, many bacteria can also be very harmful by causing disease. Understanding how to identify bacteria has numerous applications and is incredibly important for anyone planning to enter the medical field or begin a career in research. Having the background knowledge of identifying an unknown bacteria may one day aid healthcare professionals diagnose their patient with a particular bacterial infection or help researchers determine various clinical, agricultural, and numerous other uses for bacteria.
Bauman, R. W., & Masuoka, E. (2009). Microbiology: with diseases by body system (3rd ed.). San Francisco: Pearson Benjamin Cummings.
During her presentation, Bonnie Bassler expends many different aspects of communication in a bacterial level and how successful communication is important to survival and efficient functioning. It is evident from her presentation that without a common language to communicate with, bacteria could not work as efficiently as they do, neither to immunize people nor create havoc in them. In her presentation, Bassler asserts the importance of the use of language in communication in bacteria, using rhetorical appeals – logical, ethical and emotional – and how it is consequently related to humans.
Slack, John M. and I. S. Snyder. Bacteria and Human Disease. Chicago: Year Book Medical Publishers, Inc., 1978.
These germs, or organisms, are often living in or on our bodies. Most of the time they are harmless, and sometimes they are helpful.2 These organisms are often called microorganisms because they are so small they can only be seen through a microscope. There are millions of microorganisms living in your body that help with body functions. The problem is when a microorganism that can cause disease enters your body and starts to multiply. These unwanted microorganisms are called pathogens. Pathogens can be bacteria, viruses, fungi, protozoans and insects or animals.3
“But how did it come to this?” you’re probably asking yourself. Humans may have been studying antibiotics, but so were bacteria – and they’ve b...
What is viral infectious disease? Generally, viral infectious disease is contagious disease cause by the virus. Wide range of virus will cause viral infectious disease. Viral infectious disease had a very big impact on the practice of dentistry as viruses can spread easily in various routes of transmission, highly contagious and many prevention need to be taken in providing treatment to patient with viral infectious disease. Some of the famous and notable viruses will be in the family herpexviridae, paramyxoviridae, adenoviridae and retroviridae. However, in here, we will only focus on Acquired Immunodeficiency Syndrome (AIDS).
Unit 5.3 Task 1 The germ theory of disease is the theory that microscopic organisms such as bacteria and viruses can cause infectious diseases. The theory was confirmed by Louis Pasteur in the 1800’s. Once germ theory was discovered, it started the development of sanitation and aseptic techniques. Also, due to Louis Pasteur’s work with fermentation and broths, he developed pasteurization for beverages such as milk and wine.
Yersinia is a genus of bacteria that belongs to the Enterobacteriaceae family (Adams and Moss 2010). It is a rod-shaped, gram-negative, facultative anaerobic, non-spore forming, catalase-positive and oxidase-negative bacterium (Adams and Moss 2010). The genus was named after Alexandre Yersin who was a French bacteriologist in 1894 (Adams and Moss 2010). There are three species in the genus Yersinia: Yersinia enterocolitica, Yersinia pseudotuberculosis and Yersinia pestis (Wellcome Trust Sanger Institute 2014). Y. enterocolitica is the most common in the United States where it occurs mainly in children (Centers for Disease Control 2009). It is known for growing at a range temperature of -1℃ to + 40℃ (Adams and Moss 2010). This bacterium is heat sensitive with a lot of variation between strains (Adams and Moss 2010). The pH it usually thrives best in is at pH 7-8 with a minimum between 5.1-4.1(Adams and Moss 2010). Y. enterocolitica can be isolated mainly from soil, water and animals (Adams and Moss 2010). The bacterium is divided in categories such as biotypes stereotypes, and phage type (Adams and Moss 2010).
Microbes are microscopic life forms, usually too small to be seen by the naked eye. Although many microbes are single-celled, there are also numerous multi-cellular organisms. The human body has 10-100 trillion microbes living on it, making it one giant super-organism. Since the first link between microbes and diseases was made, people have been advised to wash their hands. Scientists, however, have recently started to investigate more closely how the microbes that call the human body home affect our health. While some microbes cause disease, others are more beneficial, working with our bodies in many subtle ways.
But beneficial to forensic microbiologist by using genetic engineering on themselves they can solve a case of biological crimes quickly. These scientists use their knowledge of life to protect it (Mackay Pg.86). There is a field within forensic science that focuses on the study if microscopic organism. This is because microscopic organisms can be used to commit a crime (Pg. 74). There was once a huge threat of biological weapons that posed a threat to modern society in Tokyo. When a well thought out plan with germs had affected the people on a train had caused symptoms such as coughing, choking, and vomiting. The substance used in Tokyo train can kill people within 10 minutes after inhaled (Pg. 76). “The potential of Biological weapons being used is greater than at any other time in history”. Quote by Microbiologist Bruce Budowle and Collages. (Pg.78). Some diseases caused by organisms that is classified as potential biological weapons occurs from time to time. A forensic microbiologist will come to evaluate a community when multiple people in that community catch a serious medical symptoms. They will come to determine if there is a reason to suspect that a bioterrorist crime has taken place (Pg. 79). An important to find out is to correctly identify the pathogen a disease producing agent is to use a suspicious outbreak so that people infected can
Handling and caring for rodents (including hamsters and gerbils) or even fish, puts humans at risk for the below-mentioned infections.