Aim: To study the effect of substrate concentration on the activity of the enzyme catalase, which is found in living tissues and cells. In this experiment we will obtain catalase from yeast cells. The substrate will be hydrogen peroxide (H202), which vary concentrations from 1-10%. We will measure the rate of reaction by the volume of oxygen (02) released into the water. Catalase will do this reaction: 2H2O2 2 H2O2 + O2
Catalase Activity Experiment Aim: to find out if different concentration of hydrogen peroxide release different amounts of oxygen when in contact with catalase enzyme. Risk assessment: Hydrogen peroxide is corrosive and can therefore irritate the skin and damage the eye if it comes into contact with these. To prevent this extra caution must be taken to avoid contact with the skin and goggles should be worn while carrying out the experiments. Other sharp implements such as the scalpel
At the time of conducting the experiment, the intention was to analyse the effect that an increase in catalase concentration had on the amount of oxygen (O2) produced if the hydrogen peroxide (H2O2) concentration was constant. It was proposed that when the catalase increases in concentration at the increments of 0.5%, 1%, 2%, and 4% and the hydrogen peroxide remained at a fixed concentration, the oxygen production would rise gradually. It was suggested that this would occur due to the collision theory
the concentration of the enzyme just to name a few. This experiment was conducted to show the effects of different pH levels on the activity of the catalase enzyme, the data obtained in this experiment supports the initial hypothesis of the experiment which states that the catalase enzyme will function optimally at a pH of 7 and efficiency will decrease as the pH of solutions moves further away from 7. This is true because in the experiment the test tube containing the distilled water which has a
Term 1, 2017 Grade 11 Biology In this report we will discuss the rate in which catalase, a type of enzyme, reacts with hydrogen peroxide to create oxygen. We measured the rate that oxygen filled up a glass test tube filled with water, adding more catalase to the hydrogen peroxide to see if oxygen would be made faster. Enzymes are biological molecules that are a catalyst for chemical reactions within cells, they are made out of large proteins. Without enzymes these chemical reactions
communicate with each other by keeping the life and death of the cell under control. What is the substrate of catalase? A substrate is a substance in which an enzyme reacts. A catalase is an enzyme that destroys harmful substances inside your body. Hydrogen peroxide (H_2 O_2) is produced in the body because of the reactive nature of oxygen. Oxygen atoms that are lost can be used to produce this. Catalase converts H_2 O_2 into the water and oxygen gas, thus, protects the DNA. Describe three safety factors
Comparing the Reaction Rates Between Potato and Hydrogen Peroxide Against Liver and Hydrogen Peroxide Through Loss in Mass Background information: Catalase is an enzyme that is found in all cells. This means that it is an intracellular enzyme. And enzyme is a biological catalyst. A catalyst is some thing that speeds up a reaction without being changed itself. Because of this enzymes and catalysts can be used again and again. Enzymes are protein chains that have a primary, secondary and
Temperature for Potato Catalase Introduction In this experiment. the optimal temperature of catalase in potatoes will be determined using its substrate hydrogen peroxide solution. The reaction of catalase in hydrogen peroxide solution will change depends on the temperature of the hydrogen peroxide solution. If the reaction time of catalase is related to the temperature of its substrate, when the temperature of hydrogen peroxide reaches to 35-40˚C, the reaction of the catalase will be the fastest which
Catalase is a common enzyme that is produced in all living organisms. All living organisms are made up of cells and within the cells, enzymes function to increase the rate of chemical reactions. Enzymes function to create the same reactions using a lower amount of energy. The reactions of catalase play an important role to life, for example, it breaks down hydrogen peroxide into oxygen and water. Our group developed an experiment to test the rate of reaction of catalase in whole carrots and pinto
enzyme catalase, which can be found in bovine liver and yeast. Our hypothesis regarding enzyme activity is that the aspects of biology and environmental factors contribute to the different enzyme activities between bovine liver and yeast. The purpose of this study is to analyze the activity of the enzyme, catalase, through our understanding
will the enzyme catalase in the potato sample catalyze hydrogen peroxide most effectively? 2. Will the amount of hydrogen peroxide in relation to the amount of potato sample affect my results? 3. Will crushing the potato sample lead to different results, than if a whole potato is used? 4. What is the optimum pH for the enzyme catalase? 5. Will the enzyme catalase actually catalyze hydrogen peroxide in an alkaline medium? 6. Hypothesis I predict that the enzyme activity of catalase on hydrogen peroxide
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction. This is a type of reaction where a molecule is broken down into smaller pieces. It is called an anabolic
Investigating a Factor that Affects Enzyme Activity Planning -------- Aim --- To investigate a factor which will affect the activity of catalase, whilst keeping all variables constant. Possible Independent Variables ------------------------------ Here are a number of possible independent variables that could be changed in the experiment: Independent variable Continuous/Discontinuous Easy to measure? Volume of substrate used Continuous Yes Type of enzyme
The Rate at Which It is Broken Down by Catalase AIM To find out whether the concentration of Hydrogen Peroxide affects the rate of decomposition of hydrogen peroxide when broken down by catalase. SCIENTIFIC KNOWLEDGE Hydrogen peroxide is a liquid bi-product of many chemical reactions in living things. It is toxic, so has to be broken down by the enzyme catalase which is also found in most living things. E.g. [IMAGE]Hydrogen peroxide catalase water + oxygen 2H2O2 (aq) 2H2 O +
To investigate the amount of oxygen foam (cm) produced by the enzyme catalase when it breaks down hydrogen peroxide in the animal (liver, milk, honey) and plant cells(potato, purple cabbage) into oxygen and water Motivation: The reason I chose this topic was because I wanted to find out what enzymes are used for, to what extent they are used in our bodies and, how vast are their applications in food industries. Enzymes have been used in research, mainly because of their ability to facilitate reactions
Measure Rate of Conversion of Hydrogen Peroxide to Water and Oxygen Overview: In this laboratory you will use a chemical titration to measure and then calculate the rate of conversion of hydrogen peroxide (HO) to water and oxygen using the enzyme catalase. Objectives: Before doing this exercise, you should understand: Ø the general functions and activities of enzymes Ø the relationship between the structure and function of enzymes Ø the concept of initial reaction rates of enzymes Ø how the
Effect of different size beads on the activity of immobilised catalyse Aim: To find out how varying the size of beads containing yeast varies the rate of activity of catalase in a fermentation reaction of hydrogen peroxide with immobilised enzymes. A catalyst is a substance that speeds up the rate of a chemical reaction, without itself being used up in the process however it maybe affected physically i.e. degradation of the surface of the catalyst. Catalysts work by reducing the activation
Name: Stuart Edwards Topic: The effect of pH and temperature on the enzyme catalase. One Research Task: Proposal Draft 2 St. Stithians Boys College Content Rationale Pg. 2 Concept diagram Pg.2 Literature review Pg.3 Topic
Biological Effects on Potato Potatoes, like all other vegetables contain the enzyme catalase, which breaks down the toxic waste product of hydrogen peroxide. Potatoes have an unusual response to long exposures of light, where the chemical properties are altered within the cells. This can be seen as the surface changes green in colour. Storage conditions of potatoes are therefore important, to ensure potatoes remain in the original state after cultivation. Within this assessment, the effects
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells Introduction Enzymes are made of proteins and they speed up reactions, this means that they act as catalysts. Hydrogen peroxide is a byproduct of our cell's activities and is very toxic. The enzymes in our bodies break down the hydrogen peroxide at certain temperatures they work best at body temperature, which is approximately 37 degrees. At high temperatures, the cells begin to denature. This