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Hydrogen peroxide + catalase reaction
Hydrogen peroxide + catalase reaction
The effect of temperature on catalase activity
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Determine the Optimal Temperature for Potato Catalase
Introduction In this experiment. the optimal temperature of catalase in potatoes will be determined using its substrate hydrogen peroxide solution. The reaction of catalase in hydrogen peroxide solution will change depends on the temperature of the hydrogen peroxide solution. If the reaction time of catalase is related to the temperature of its substrate, when the temperature of hydrogen peroxide reaches to 35-40˚C, the reaction of the catalase will be the fastest which means it consumes the least amount of time for the reaction.
The independent variable in this experiment is the temperature of hydrogen peroxide solutions since temperature is the variable that is changed by the scientist.
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The dependent variable is the reaction time of catalase, which is the one that might be affected by the change in the independent variable. In this experiment, a focus of observation will take place on the reaction time of catalase (dependent variable) to see how it responds to the change made to the hydrogen peroxide temperature (independent variable). In addition, there are two controlled variables that must be kept constant throughout the experiment. One of the controlled variables is the type of thermometer that is used in the experiment. Since different thermometers might have a slightly different measurement, which will affect the record of temperature, all of the temperature values should be tested by the same thermometer. The other controlled variable is the size of the test tubes that used are used in the experiment. If different sizes of test tubes are used, the reaction …show more content…
This is because there is a higher chance of the substrate hydrogen peroxide binding with catalase. At a high temperature, hydrogen peroxide and the potato catalase are supplied with more kinetic energy so that they move around at a higher speed. As they move at a higher speed, collisions between the substrate and the enzyme take place more frequently, so more enzyme-substrate complexes are formed which produces oxygen. But, when the temperature gets to around 45˚C, the increase of temperature cause the rate of catalase reaction decreases. This is because heat provided +too much energy so that each individual atom vibrated rapidly. Too much amount of energy can break the bonds between the atoms in the enzyme so that the shape of enzyme active site changed. Since the enzyme active site is specific to the substrate, the enzyme cannot bind with the substrate anymore which results in a decrease of the rate of
For example, substrate concentration, enzyme concentration, and temperature could all be factors that affected the chemical reactions in our experiment. The concentration of substrate, in this case, would not have an affect on how the bovine liver catalase and the yeast would react. The reason why is because in both instances, the substrate (hydrogen peroxide) concentration was 1.5%. Therefore, the hydrogen peroxide would saturate the enzyme and produce the maximum rate of the chemical reaction. The other factor that could affect the rate of reaction is enzyme concentration. Evidently, higher concentrations of catalase in the bovine liver produced faster reactions, and the opposite occurs for lower concentrations of catalase. More enzymes in the catalase solution would collide with the hydrogen peroxide substrate. However, the yeast would react slower than the 400 U/mL solution, but faster than the 40 U/mL. Based on this evidence, I would conclude that the yeast has a higher enzyme concentration than 40 U/mL, but lower than 400
In both solutions of catalase there is a steady increase in reaction relative to the hydrogen peroxide concentration as it increases. A significant jump is observed in the carrot catalase solution between .25% and .5% whereas the pinto bean catalase solution has a steady increase. Each solution doesn’t generate much more reaction to the next increment of hydrogen peroxide concentration, 1%. In general it stayed level. This continued to be a trend for the pinto bean catalase solution, plateauing through to the 6% concentration of hydrogen peroxide. This is known as the point of saturation.
Input variables In this experiment there are two main factors that can affect the rate of the reaction. These key factors can change the rate of the reaction by either increasing it or decreasing it. These were considered and controlled so that they did not disrupt the success of the experiment. Temperature-
This experiment was conducted to determine the effects of pH and temperature on peroxidase from a potato. The optimum temperature for peroxidase was determined to be 23°C, because it had a rate of absorbance of 0.3493, higher than the other temperatures evaluated. A temperature of 48°C is inefficient of speeding up peroxidase activity because its rate of absorbance was 0.001.
This happens when the temperature is too high; the process is called “denaturing”. When an enzyme reaches a certain temperature, it will have so much energy that it is de-shaped; it is “denatured”. This diagram shows how a denatured enzyme will not work: [IMAGE] The enzymes will hardly work at very low temperatures (they wont be
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
I have chosen to vary the concentration of the enzyme catalase, as it is simple to do in the laboratory, and will obtain easy to interpret results. Therefore, all the other variables will be kept constant to make sure the experiment is fair. Keeping the experiment fair: All of the variables with the exception of the concentration of catalase will remain the same, to make sure that the results obtained are not influenced by anything other than the concentration.
== == == = This is what I'm going to be changing in the experiment and this will be the temperature and the concentration of the yeast. There are several variables in this experiment, they are: · Amount Used - Too much or too little of the hydrogen peroxide causes the reaction to speed up/slow down producing different amounts of oxygen.
The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells Introduction Enzymes are made of proteins and they speed up reactions, this means that they act as catalysts. Hydrogen peroxide is a byproduct of our cell's activities and is very toxic. The enzymes in our bodies break down the hydrogen peroxide at certain temperatures they work best at body temperature, which is approximately 37 degrees. At high temperatures, the cells begin to denature. This means that the hydrogen peroxide is prevented from being broken down because they will not 'fit' into the enzyme.[IMAGE] Objective I am going to find out how the concentration of the substrate, hydrogen peroxide affects the reaction in the catalase inside the potato cells.
The aim of my investigation is to find out whether the increase of temperature increases the rate of reaction between the two reactants of Sodium Thiosulphate and Hydrochloric acid. I will then find out and evaluate on how temperature affects this particular reaction. Factors There are four main factors, which affect the rate of reaction that are considered as variables for the experiment I will be doing, they are the following: Molecules can only collide when two of them meet together.
Independent variables: The temperature of hcl gas will be decreased and increased throughout the experiment.
To make the test fair, the following parameters must remain constant during the experiment. These parameters must remain constant during the experiment. These parameters are water, Hydrogen Peroxide, Catalyse and the duration of the reactions. By insuring the test is fair, we will gain accurate results. Variables Dependant Variable: Time, Size of 5cm piece of Potato Independent Variable: Amount of Oxygen released Control Variable: Volume of Hydrogen peroxide, size of Potato, concentration of Hydrogen Peroxide Hypothesis I predict that the breakdown of Hydrogen Peroxide will be quicker when the surface area is increased.