5. Problems:
1. In which pH will the enzyme catalase in the potato sample catalyze hydrogen peroxide most effectively?
2. Will the amount of hydrogen peroxide in relation to the amount of potato sample affect my results?
3. Will crushing the potato sample lead to different results, than if a whole potato is used?
4. What is the optimum pH for the enzyme catalase?
5. Will the enzyme catalase actually catalyze hydrogen peroxide in an alkaline medium?
6. Hypothesis
I predict that the enzyme activity of catalase on hydrogen peroxide will be more effective in an acidic pH and less effective in an alkaline pH over a period of 10 minutes.
7. Aim
To determine the effects that the different pH levels of water, vinegar and oven cleaner will have on the enzyme catalase while it catalyzes hydrogen peroxide.
8. Variables
8.1 Dependent variables
The time taken for the enzyme catalase to catalyze hydrogen peroxide (until there are
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My hypothesis was incorrect, as catalase did not catalyze hydrogen peroxide the most effectively in an acidic pH. In the experiment we saw that catalase in the alkaline solution catalyzes hydrogen peroxide the least effectively, because it had almost no bubbles, which means that the hydrogen peroxide was not broken down into water and oxygen. The catalase in the acidic solution catalyzes a small amount of the hydrogen peroxide as there were a few bubbles, which means that the hydrogen peroxide was not completely broken down into oxygen and water. Therefore it was not very effective. The catalase in the neutral solution catalyzes hydrogen peroxide the most effectively, because it produced the most bubbles and bubbled for the longest period of time, which means that the hydrogen peroxide was completely broken down into water and oxygen. Therefore we can conclude that the enzyme catalase works best in a neutral pH and least effectively in an acidic and alkaline
After conducting this experiment and collecting the data I would have to say that the optimal temperature for enzyme activity would have to be room temperature which in my experiment was thirty-four degrees Celsius. I came to this answer because the glucose test strip showed that at room temperature there was more glucose concentration that at either of the other temperatures. Due to temperature extremes in the boiling water the enzymes could no longer function because the breakdown of lactose stopped. The cold water also hindered the breakdown of the lactose but as the water warmed the enzymes were more active which can be seen in the results for the cold water at 20 minutes B. Describe the relationship between pH and the enzymatic activity of lactase.
The purpose of this study is to analyze the activity of the enzyme, catalase, through our understanding
Catalase is a common enzyme that is produced in all living organisms. All living organisms are made up of cells and within the cells, enzymes function to increase the rate of chemical reactions. Enzymes function to create the same reactions using a lower amount of energy. The reactions of catalase play an important role to life, for example, it breaks down hydrogen peroxide into oxygen and water. Our group developed an experiment to test the rate of reaction of catalase in whole carrots and pinto beans with various concentrations of hydrogen peroxide. Almost all enzymes are proteins and proteins are made up of amino acids. The areas within an enzyme speed up the chemical reactions which are known as the active sites, and are also where the
The alternate hypothesis is that there exists an optimal pH level for catecholase enzyme in which the catecholase enzyme can operate with the highest possible
The effect of a change in PH on enzymes is the alteration in the ionic
Peroxidase activity’s optimum pH was found to be pH 5, since the absorbance rate was the highest at 0.3493. Little activity occurred at pH 3, but the absorbance of the reaction with pH 7 rose steadily to 0.99. The rate of absorbance for peroxidase with pH 9 was 0.0097; pH 9 is incapable of accelerating enzyme activity. This suggests that an alkaline pH is inferior to an acidic pH in increasing peroxidase activity, and that the higher the pH level, the poorer the pH boosts the reaction. A highly acidic pH also reduces
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
= I predict that if the concentration is high in the yeast then the speed of oxygen produced in the reaction with hydrogen peroxide will also be high. This is because the amount of yeast that can react with the hydrogen peroxide can get no higher and will have the maximum affect on the reaction. If the concentration is more in favour of water then the amount of oxygen produced will be slow because there is not as much yeast to react with the hydrogen peroxide, giving less oxygen. If the temperature is not in favour of the limits to the yeast then the amount of oxygen produced will be small because the enzyme will have denatured. If the temperature is in favour of the yeast then the amount of oxygen produced will be high because it is at the prime temperature for the yeast to react.
The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells
Many factors, for example, pH and temperature affects the way enzymes work by either increasing the rate or determining the type of product produced (). The report, therefore, analyses the effects of the enzyme peroxidase in metabolic reactions and determining its optimum temperature in the reactions.
After that, from pH 9 to pH 10.2 we can see that the m.r.r. drastically decreases but still remains higher than all the rest m.r.r before pH 9. Discussion: The results of this experiment prove my hypothesis that the pH of the catalase will alter the function of the enzyme, thus the rate of reaction will change. They also prove my other hypothesis that there is a specific pH level in which the catalase works best. However, after looking at Figure 4 we can see that that optimum pH is 9 which, as mentioned in the background knowledge section, is not what would usually be the optimum pH for catalase when conducting this experiment but would be around pH 7.
The first experiments investigate the order of reaction with respect to the reactants; hydrogen peroxide, potassium iodide and sulphuric acid by varying the concentrations and plotting them against 1/time. An initial rate technique is used in this experiment so ‘the rate of reaction is inversely proportional to time.’ To find the order of reaction in respect to the reactants, 1/time is plotted against the concentration of Hydrogen Peroxide using the equation: