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Essay of hydrogen peroxide
Essay of hydrogen peroxide
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As it can clearly be seen in Figure 4, changes in Ph (independent variable) lead to significant changes in the mean rate of reaction (dependent variable. It can be observed that when pH increases from 2 to 5 the mean rate of reaction (m.r.r.) increases but not to a great extend, going from 0.054%/20sec to 0.080%/20sec. When the pH goes from 5 to 7 however we can see it going down from 0.080%/20sec to 0.070%/20sec. The most interesting change however is from pH 7 to 9 where an increase of an incredible rate can be observed. From 0.070%/20sec the rate goes up to 0.271%/20sec, reaching its maximum peak at pH 9. After that, from pH 9 to pH 10.2 we can see that the m.r.r. drastically decreases but still remains higher than all the rest m.r.r before pH 9. Discussion: The results of this experiment prove my hypothesis that the pH of the catalase will alter the function of the enzyme, thus the rate of reaction will change. They also prove my other hypothesis that there is a specific pH level in which the catalase works best. However, after looking at Figure 4 we can see that that optimum pH is 9 which, as mentioned in the background knowledge section, is not what would usually be the optimum pH for catalase when conducting this experiment but would be around pH 7. What was the cause that made the results of this …show more content…
I started exploring the possibility of the existence of a chemical reaction between the N2 molecules and the molecules on the solution. In the presence of water, NaOH breaks down into Na + OH ions, which led me to consider the possibility of the Na released reacting with the N2 available, forming NaN3, decreasing the amount of N2 and making it more likely for O2 to reach higher numbers. NaN3 is a known catalase inhibitor, decreasing the activity of the enzyme, hence decreasing the amount of O2
Data from Table 1. confirms the theory that as the concentration of glucose increases so will the absorbance of the solution when examined with the glucose oxidase/horseradish peroxidase assay. Glucose within the context of this assay is determined by the amount of ferricyanide, determined by absornace, which is produced in a one to one ratio.1 Furthermore when examining the glucose standards, a linear calibration curve was able to be produced (shown as Figure 1). Noted the R2 value of the y = 1.808x - 0.0125 trend line is 0.9958, which is statistically considered linear. From this calibration curve the absorbance values of unknowns samples can be compared, and the correlated glucose concentration can then be approximated.
In the lab, Inhibiting the Action of Catechol Oxidase we had to investigate what type of enzyme inhibition occurs when an inhibitor is added. Catechol oxidase is an enzyme in plants that creates benzoquinone.Benzoquinone is a substance that is toxic to bacteria. It is brown and is the reason fruit turns brown. Now, there are two types of inhibitors, the competitive inhibitor and non-competitive inhibitor. For an enzyme reaction to occur a substrate has to bind or fit into the active site of the enzyme. In competitive inhibition there is a substrate and an inhibitor present, both compete to bind to the active site. If the competitive inhibitor binds to the active site it stops the reaction. A noncompetitive inhibitor binds to another region
In this experiment the enzyme peroxidase and the substrate hydrogen peroxide were not mixed initially, instead they were both placed in separate tubes and were incubated at a specific temperature, to prevent hydrogen peroxide from undergoing any reaction with peroxidase until they both acquire the required temperature.
The purpose of this study is to analyze the activity of the enzyme, catalase, through our understanding
In both solutions of catalase there is a steady increase in reaction relative to the hydrogen peroxide concentration as it increases. A significant jump is observed in the carrot catalase solution between .25% and .5% whereas the pinto bean catalase solution has a steady increase. Each solution doesn’t generate much more reaction to the next increment of hydrogen peroxide concentration, 1%. In general it stayed level. This continued to be a trend for the pinto bean catalase solution, plateauing through to the 6% concentration of hydrogen peroxide. This is known as the point of saturation.
The affects of pH, temperature, and salt concentration on the enzyme lactase were all expected to have an effect on enzymatic activity, compared to an untreated 25oC control. The reactions incubated at 37oC were hypothesized to increase the enzymatic activity, because it is normal human body temperature. This hypothesis was supported by the results. The reaction incubated to 60oC was expected to decrease the enzymatic activity, because it is much higher than normal body temperature, however this hypothesis was not supported. When incubated to 0oC, the reaction rate was hypothesized to decrease, and according to the results the hypothesis was supported. Both in low and high pH, the reaction rate was hypothesized to decrease, which was also supported by the results. Lastly, the reaction rate was hypothesized to decrease in a higher salt concentration, which was also supported by the results.
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
Investigating Factors that Affect the Rate of Catalase Action Investigation into the factors which affect the rate of catalase action. Planning Aim: To investigate the affect of concentration of the enzyme catalase on the decomposition reaction of hydrogen peroxide. The enzyme: Catalase is an enzyme found within the cells of many different plants and animals. In this case, it is found in celery.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells
Materials used in the experiment included 5-7 g of the potato tissue, 50ml of 2.0M phosphate buffer coffee filter and guaiacol dye.
I will explain how it was caused and what can be done to prevent such
= = pH 1 2 3 Average Rate of Reaction (cm3/s): 0 - 0. 3 0 0 0 0 0.000 5 0 0 0 0
When the pH was at 10.4 you saw a greater increase then when the pH was at 4.4. The goal of the experiments of Enzyme Kinetics 1 and Enzyme Kinetics 2 were to determine the enzyme activity of alkaline phosphatase. Week one we determined the Vmax and KM (Figure 1). Week 2 we determined if changing the PH level were to cause an effect of the efficiency of the enzyme (Figure
The purpose of this study was to isolate, characterize, and identify an unknown species of bacteria collected from soil in Flagstaff, Arizona. The environmental isolate (EI) was found to be non-motile, this limits the bacteria from spreading across an area without outside forces. The EI had a positive reaction to the catalase test this indicates that the bacteria can convert harmful hydrogen peroxide into water and free oxygen (Shand and Fitchett 2017). It was also discovered that the EI was a strict aerobe which is significant because it cannot live without oxygen. This limits the area the bacteria can survive in. It was discovered that the EI was predominantly arranged in clusters and had the ability to produce a biofilm. This