The Concentration of Hydrogen Peroxide and Speed of The Rate at Which It is Broken Down by Catalase AIM To find out whether the concentration of Hydrogen Peroxide affects the rate of decomposition of hydrogen peroxide when broken down by catalase. SCIENTIFIC KNOWLEDGE Hydrogen peroxide is a liquid bi-product of many chemical reactions in living things. It is toxic, so has to be broken down by the enzyme catalase which is also found in most living things. E.g. [IMAGE]Hydrogen peroxide catalase water + oxygen 2H2O2 (aq) 2H2 O + O2 [IMAGE] The enzyme catalase (peroxidase) was the first enzyme discovered and is one of the fastest enzymes, its turnover rate is about 40,000 molecules/second. It speeds up the decomposition of hydrogen peroxide. · Enzymes are not used up by the reaction and remain unchanged. · Enzymes work most efficiently at their optimum temperature and optimum pH, which from a previous experiment, I know to be about pH8 · Can be denatured (changed in shape) by excess heat (+45°C) and/or extremes of pH. · Very specific to their substrate molecule. THEORY COLLISION THEORY Enzymes are biological catalysts. They tend to speed up the rate of biochemical reactions. Decomposition of hydrogen peroxide [IMAGE]2H2O2 (aq) 2H2 O + O2 The enzyme catalase (peroxidase), catalase was first enzyme discovered. It is one of the fastest enzymes, with a turnover rate of about 40,000 molecules/second. Speeds up the rate of decomposition of hydrogen peroxide [IMAGE] [IMAGE] [IMAGE][IMAGE][IMAGE] active site [IMAGE] catalase hydrogen peroxide ... ... middle of paper ... ...2 9 2 5 2.5 11 2 5 3 12 2 2.5 0.5 2 2 2.5 1 2 0 2.5 1.5 3 1 2.5 2 3 0 2.5 2.5 4 1 2.5 3 4 0 Conclusion This experiment was far more accurate than the previous one, however the results were still well off what would be expected for 25vol. This leads me to believe that something else is affecting that experiment. For the second experiment all of the hydrogen peroxide concentrations were made up at the same time, so this would not have been a factor. The first three results were just about perfect, each doubling. This shows that whatever it is that is causing the change in the pattern, only affects the strongest concentrations.
However, at 3% substrate concentration, the hydrogen peroxide decomposition showed an immediate peak of up to 3.8 mm in height. As the substrate concentration slowly increased, enzyme
The purpose of this study is to analyze the activity of the enzyme, catalase, through our understanding
In both solutions of catalase there is a steady increase in reaction relative to the hydrogen peroxide concentration as it increases. A significant jump is observed in the carrot catalase solution between .25% and .5% whereas the pinto bean catalase solution has a steady increase. Each solution doesn’t generate much more reaction to the next increment of hydrogen peroxide concentration, 1%. In general it stayed level. This continued to be a trend for the pinto bean catalase solution, plateauing through to the 6% concentration of hydrogen peroxide. This is known as the point of saturation.
The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
To make this a fair test, we need to keep the same all variables that
A rate is a measure of change that occurs in a given time whilst a
placed in each tube. Each tube was then placed in a water bath of the
Investigating the Amount of Oxygen Given Off When Catalase Reacts with Hydrogen Peroxide My aim for this investigation is to measure the amount of oxygen given off when we react catalase (enzyme) with hydrogen peroxide (substrate), this means that I aim to investigate the effect of hydrogen on the activity of catalase. Background Information Enzymes such as Catalase are protein molecules that are found in living cells. They are used to speed up specific reaction in the cell. The enzymes are all very specific as each enzyme performs one particular reaction.
It is important however to note that the NH4 and K ions are still in
-they were actually separated from each other for (an assumed) few years, not a few days
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells Introduction Enzymes are made of proteins and they speed up reactions, this means that they act as catalysts. Hydrogen peroxide is a byproduct of our cell's activities and is very toxic. The enzymes in our bodies break down the hydrogen peroxide at certain temperatures they work best at body temperature, which is approximately 37 degrees. At high temperatures, the cells begin to denature. This means that the hydrogen peroxide is prevented from being broken down because they will not 'fit' into the enzyme.[IMAGE] Objective I am going to find out how the concentration of the substrate, hydrogen peroxide affects the reaction in the catalase inside the potato cells.
Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide
This graph shows the result that I expect to get, I expect to see a