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Surface area and enzymes
Importance of enzymes in living organisms
Effects of concentration on enzymes
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Comparing the Reaction Rates Between Potato and Hydrogen Peroxide Against Liver and Hydrogen Peroxide Through Loss in Mass
Background information:
Catalase is an enzyme that is found in all cells. This means that it
is an intracellular enzyme. And enzyme is a biological catalyst. A
catalyst is some thing that speeds up a reaction without being changed
itself. Because of this enzymes and catalysts can be used again and
again. Enzymes are protein chains that have a primary, secondary and
tertiary structure. Their primary structure shows the order and types
of amino acids used to form the protein chain. The secondary structure
shows the basic folding of the protein and is held in place by
hydrogen bonds. The tertiary structure shows a more complex folding
which gives it its globular shape. The tertiary folding of the enzyme
also gives it its active site. The active sit of an enzyme is the part
of the enzyme that determines what the enzyme will react with. If this
active site is destroyed in any way the enzyme is said to be denatured
and will no longer work. Certain things affect the reaction rate of a
substance where an enzyme is used. The concentration of the reactant
will affect the reaction rate. If there is a strong concentration of
reactant or catalase then there will be a faster reaction rate than if
the concentration was weak. Also, the higher the temperature of the
substance the faster the reaction rate will be. This also applies to
the surface area of the cells containing the catalase. If the cells
have a large area the reaction rate will be very fast. This is because
more catalase will be exposed to the reactant and this will mean that
more products are being made in a short amount of time. However if the
cells have a small surface area then the reaction rate will be slow
because less of the enzyme will be accessible to the product and
therefore fewer products will be made in a certain amount of time.
Catalase reacts with hydrogen peroxide to form water and oxygen in the
Whole carrots have a different reaction to the higher concentrations of hydrogen peroxide. There is a dramatic increase in the rate of reaction of catalase enzymes in the whole carrot, meaning that the saturations kinetics can be utilized at much higher rates of concentration.
However, at 3% substrate concentration, the hydrogen peroxide decomposition showed an immediate peak of up to 3.8 mm in height. As the substrate concentration slowly increased, enzyme
When this substrate fits into the active site, it forms an enzyme-substrate complex. This means that an enzyme is specific. The bonds that hold enzymes together are quite weak and so are easily broken by conditions that are very different when compared with their optimum conditions. When these bonds are broken the enzyme, along with the active site, is deformed, thus deactivating the enzyme. This is known as a denatured enzyme.
called an active site. This active site is made by a few of the amino
Observations: Liver and Potatoes will be placed in hydrogen peroxide in order to observe the reactions due to the enzyme, catalase, found in both.
shape. The sand is a sand. Their hydrophilic side-chains on the outside of the molecule. make them soluble in water. Enzymes can catalyze both anabolic and catabolic reactions within an organism.
Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide
The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells Introduction Enzymes are made of proteins and they speed up reactions, this means that they act as catalysts. Hydrogen peroxide is a byproduct of our cell's activities and is very toxic. The enzymes in our bodies break down the hydrogen peroxide at certain temperatures they work best at body temperature, which is approximately 37 degrees. At high temperatures, the cells begin to denature. This means that the hydrogen peroxide is prevented from being broken down because they will not 'fit' into the enzyme.[IMAGE] Objective I am going to find out how the concentration of the substrate, hydrogen peroxide affects the reaction in the catalase inside the potato cells.
The three-dimensional contour limits the number of substrates that can possibly react to only those substrates that can specifically fit the enzyme surface. Enzymes have an active site, which is the specific indent caused by the amino acid on the surface that fold inwards. The active site only allows a substrate of the exact unique shape to fit; this is where the substance combines to form an enzyme- substrate complex. Forming an enzyme-substrate complex makes it possible for substrate molecules to combine to form a product. In this experiment, the product is maltose.
Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is important that a specific enzyme is present during the process. For example, lactase must be able to collaborate with lactose in order to break it down (Madar & Windelspecht, 105).
Changes in pH lead to the breaking of the ionic bonds that hold the tertiary structure of the enzyme in place. The enzyme begins to lose. its functional shape, particularly the shape of the active site, such. that the substrate will no longer fit into it, the enzyme is said to. be denatured.
The type seen throughout the human body involve enzyme catalysis. Enzymes are present throughout many key bodily processes and keep the body from malfunctioning. An enzyme catalyzes a reaction by having the substrate bind to its active site.2 This is known as the Lock and Key Theory, which states that only the correctly oriented key (substrate) fits into the key hole (active site) of the lock (enzyme).2 Although this theory makes sense, not all experimental data has explained this concept completely.2 Another theory to better accurately explain this catalysis is known as the Induced-Fit Theory.2 This theory explains how the substrate determines the final form of the enzyme and shows how it is moderately flexible.2 This more accurately explains why some substrates, although fit in the active site, do not react because the enzyme was too distorted.2 Enzymes and substrates only react when perfectly aligned and have the same
Therefore, it takes part as a co-factor in many enzymatic reactions, and also acts as a plasma localized anti-oxidant molecules (Farbstein, 2010).
The first experiments investigate the order of reaction with respect to the reactants; hydrogen peroxide, potassium iodide and sulphuric acid by varying the concentrations and plotting them against 1/time. An initial rate technique is used in this experiment so ‘the rate of reaction is inversely proportional to time.’ To find the order of reaction in respect to the reactants, 1/time is plotted against the concentration of Hydrogen Peroxide using the equation: