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Effect of surface area on catalase activity1
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Surface Area's Effect on the Way Enzymes Work Introduction I am doing an investigation to see how the amount of surface area affects how a enzyme, Catalase works. Catalase is a biological catalyst which means that is speeds up a reaction itself. I am going to see how much foam is created after 5 minutes, this should show us whether the amount of surface gives a better or worse reaction. My input variable will be the surface area. The potato which contains the Catalase will be cut into piece this size: 1cm x 1cm x 2cm. Test tube A will have a piece of potato which is this size. Test tube B will have this size potato in but cut in half and Test tube C will have this size potato in it but cut into thirds, this means the surface area is gradually increased. The surface area for the potato in Test Tube A = 10cm B = 12cm C = 14cm This shows that there is a difference of 2cm between A and B, and B and C, also that there is a 4cm difference between A and C. Test tube B has increased by one fifth of what Test tube A is and Test tube C has increased by two fifths of what test tube A is. Prediction The reaction that I expect to happen is: 2H2O2 2H2O + 2O2. I would expect that as the surface area increases so will the amount of foam created. This is because the more surface area is the more chance there is that the molecules will collide with each other. Whereas if there is less surface area there is less to collide with. If there was less surface area then there would be a less likely chance that the enzymes would meet their substrates so they could react. I expect my graph to look like it does on the next page because I would expect that because the surface area for Test tube B has
5. A second test tube was then filled with water and placed in a test
We then took 1ml of the 1% solution from test tube 1 using the glucose pipette and added it to test tube 2, we then used the H2O pipette and added 9ml of H2O into test tube 2 creating 10ml of 0.1% solution
I will get 5 test tubes and place them into the test tube rack. I will
3.) Divide your 30g of white substance into the 4 test tubes evenly. You should put 7.5g into each test tube along with the water.
Put a label on each test tube. With a pencil, number each test tube from one to twelve.
If we say that the right hand side in picture 1 is the potato, and the
* Size of potatoes * Diameter of each potato tube * Time in sugar solution We need to make sure in both experiments the fair test lists are used and the procedures are carried out. This needs to be done otherwise my results will not be accurate and will look odd. Method: Firstly we got out all our equipment.
Size of potato-will be the same as it will be cut using a cork and borer which cuts them all to the same diameter.
from 10cm to 50cm to make it easier to see the difference in a graph.
Knife to cut the potato to the correct shape and size for reasons. stated in the method. Tile to not score any school surfaces. Test tubes to hold the potato and solution in. Beaker to alter solution concentration.
Potato 2. Standard sugar solution (1molar) 3. Wash bottle 4. Test tubes and test - tube rack 5. Cork borer-size (4mm diameter) 6.
4. Put each group of potato discs in one of the 6 test tubes and watch
In conclusion, the method seems flawed in accurately measuring the glucose concentration of solutions. To make the experiment a "fair test," three main variables needed to be kept constant. Firstly, the volumes, including all measurements, i.e., the amount of Benedict's solution used and the amount of glucose water solution in each test tube. Secondly, the time that all the test tubes were kept in the water bath had to be the same for all test tubes, including solution X.
second test tube also add 6 mL of 0.1M HCl. Make a solution of 0.165
In my experiment, I will use an overall volume of 50 cm³ of 2moles of