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State the biological significance of lipids
State the biological significance of lipids
State the biological significance of lipids
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Structure and Function of Lipids in Plants and Animals
Lipids are the name given to a mixed group of organic compounds. The
elements Hydrogen, Oxygen and Carbon are always found in Lipids. The
difference between them and Carbohydrates is that they have a lower
proportion of Oxygen in the molecule. Lipids are insoluble in water,
but they are soluble in organic solvents, such as ether, acetone,
chloroform or benzene.
There are several parts in the group of Lipids:
o fats and oils,
o waxes,
o phospholipids,
o steroids (like cholesterol, oestrogen and testosterone), and some
other related compounds.
At room temperature, fats are solids and oils are liquids. Fats and
oils are typically found in animals and plants. Fats are of animal
origin, while oils tend to be found in plants. Fats and oils are made
up of a glycerol (a type of alcohol with a hydroxyl group on each of
its three carbons) and three fatty acids (an organic acid). Since
there are three fatty acids attached, these are known as triglycerides.
A condensation reaction produces a triglyceride and water molecule
when the glycerol and three fatty acids react together (see diagram
below). The fatty acid molecules are insoluble in water because of
their long hydrocarbon tails (the tails are referred to as hydrophobic
or “water-hating”). The head of the molecule is a carboxyl group which
is hydrophilic or “water-loving”.
[IMAGE]
Fatty acids are the main component of soap, where their tails are
soluble in oily dirt and their heads are soluble in water to emulsify
and wash away the oily dirt. However, when the head end is attached to
glycerol to form a fat, that whole molecule is hydrophobic.
The digestion of triglycerides is catalysed by lipase enzymes which
are found, for example, in the digestive system of animals and in
germinating seeds, in the lysosomes inside cells and in the secretions
of saprophytic bacteria and fungi. Triglycerides are created in fat
storage depots, in such places as, in liver cells, in the adipose
The boiling point of the product was conducted with the silicone oil. Lastly, for each chemical test, three test tubes were prepared with 2-methylcyclohexanol, the product, and 1-decene in each test tube, and a drop of the reagent were added to test tubes. The percent yield was calculated to be 74.8% with 12.6g of the product obtained. This result showed that most of 2-methylcyclohexanol was successfully dehydrated and produced the product. The loss of the product could be due to the incomplete reaction or distillation and through washing and extraction of the product. The boiling point range resulted as 112oC to 118oC. This boiling point range revealed that it is acceptable because the literature boiling point range included possible products, which are 1-methylcyclohexene, 3-methylcyclohexene, and methylenecyclohexane, are 110 to 111oC, 104oC, and 102 to 103 oC. For the results of IR spectroscopy, 2-methylcyclocahnol showed peaks at 3300 cm-1 and 2930 cm-1, which indicated the presence of alcohol and alkane functional group. Then, the peak from the product showed the same peak at 2930 cm-1 but the absence of the other peak, which indicated the absence of the alcohol
Every 5 minutes, a small amount of mixture was dissolved in acetone (0.5 mL) and was spotted onto a thin layer chromatography (TLC) plate, which contained an eluent mixture of ethyl acetate (2 mL) and hexanes (8 mL). The bezaldehyde disappearance was monitored under an ultraviolet (UV) light. Water (10 mL) was added after the reaction was complete, and vacuum filtrated with a Buchner funnel. Cold ethanol (5 mL) was added drop-by-drop to the dried solid and stirred at room temperature for about 10 minutes. Then, the solution was removed from the stirrer and place in an ice bath until recrystallization. The recrystallized product was dried under vacuum filtration and the 0.057 g (0.22 mmol, 43%) product was analyzed via FTIR and 1H NMR
Lipid-soluble and Water-soluble hormones are products of the Endocrine gland. These hormones are transported in the body through the blood stream. However, they have different characteristics or properties that ensure their effectiveness is accomplished.
As shown in figure 2, the percentage of each isomeric alcohol in the mixture had been determined. The hydrogen atom on the carbon atom with the hydroxyl group appear at around 4.0 ppm for borneol and 3.6 ppm for isoborneol. The product ratio has been determined by integrating the peaks. A ratio of 6:1 for the Isoborneol/borneol ratio was expected and is validated by the calculations shown above, with isoborneol percentage at 83.82% and 16.17% of borneol. A CHCl3 group noted at around 7ppm and a CH2Cl2 at around 3.5ppm.
Data Table 3 indicates the observations from these tests. Though a control test for each test wasn’t prepared, due to the starting reagents being unattainable, the results clearly show that the product is unsaturated. An unsaturated compound means that there is/are bonds in its structure. The product was also analyzed by infrared spectroscopy and gas chromatography. The spectrums obtained allowed one to determine the composition of 1-methylcyclohexene; any impurities and excess products were observed as well. From the infrared spectrum, there is a little peak around 3300-3500 cm-1; this indicated a very little presence of alcohol in the product and thus, most of the alcohol has been successfully removed. If one compared the IR spectrum of the product to the starting material, 2-methylcyclohexanol, one could clearly see the change in peak size of the O-H stretch. The infrared spectrum of 1-methylcyclohexene also depicted a C-H stretch and an alkene functional group at 500-1500 cm-1 and 2932.54cm-1, respectively. From the gas chromatography spectrum (Data Table 4), the area percentages show that there were three products - peaks 22 through 24 - that were formed from the dehydration experiment. Nevertheless, there are two predominant products, as shown from their high percentages, 3-methylcyclohexene (~24%) and 1-methylcyclohexene
head group is attached to one of the glycerol hydroxyls with addition to the two hydrocarbon fatty
ester, and we produce the most acid and alcohol. In bottle 3 we still have a good
The purpose of this procedure was to use the carbohydrate sucrose in a fermentation reaction in order to produce ethanol.
Once isopentyl acetate is produced, it will become important to analyze its structure and purity.
· Collect 3 cylinders, and then cut them into 6 pieces of 3 cm with a
That is why ethanol was used as a solvent during this lab, because due to its structure, it has both polar and non-polar regions. Ethanol has a polar alcohol end capable of dipole-dipole interactions and hydrogen bonding, but also has a relatively non-polar CH3CH2- end with mostly London dispersion forces (LDFs). The general rule for solubility is, “like dissolves like,” meaning that polar solutes will dissolve in polar solvents and non-polar solutes will dissolve in non-polar solvents. Once extracted, performing a simple separation known as thin layer chromatography (TLC), the extract can be verified to see if it contains eugenol. TLC uses ethyl acetate (a polar solvent) in order to dissolve the molecules (also polar) in the extract. The polar solvent also contains hexane (a non-polar solvent) which allows for the polar molecules to precipitate out as small spots on the TLC plate. As shown in Figure 4, the solvent will move up the TLC plate until it reaches the spotted samples: cloves extract (Our), nutmeg extract (Oth), and 10 mg/mL eugenol standard (s). Several spots on the TLC plate show the interaction between molecules in the extract and the silica on the surface of the TLC plate. If the molecules in the extract exhibit more non-polar characteristics, they will get pulled further up the
Fats contain 3 fatty acids, each of which are connected to a single glycerol molecule through an ester linkage (a bond between carboxyl and hydroxyl). The resulting fat is triacylglycerol sometimes called triglyceride.
Ethanol is a clear, colorless, flammable liquid. Other names that ethanol can also be known as include ethyl alcohol, ethyl hydrate, ethyl hydroxide, fermentation alcohol, methylcarbinol, and grain alcohol. The condensed structural formula for ethanol is C2H5OH, and the expanded structural formula is shown in figure 1. This chemical compound belongs to the alcohol function group therefore characterized by the hydroxyl group –OH combined with a carbon atom. Some characteristics of ethanol include a melting point at -114.1°C, boiling point at 78.5°C and a flash point at 16.6°C. Since it has a low freezing point it is used in thermometer for temperatures below -40°C, and also in antifreeze. Ethanol is the product of fermentation of carbohydrates, in the absence of air. The carbohydrates include crops like corn, wheat, grain, and barley, which makes ethanol quite economic friendly. This chemical compound is used in many processes and is found in many everyday products.
The process of alcoholic fermentation begins with the use of enzymes. The enzymes begin to break down the long chains in starch molecules, a polysaccharide that consists of a large quantity of glucose molecules (C6H12O6) joined by glycosidic bonds as seen in figure 1, into single glucose molecules, a monosaccharide with six carbons and five hydroxyl groups. After the starch has become sugar, the enzymes are used once again, this time to convert the sugars into ethyl alcohol and carbon dioxide, CO2, as seen in figure 2 (World of Scientific Discovery, 2007). The carbon dioxide produced is released into the atmosphere, leaving water and ethanol, the alcohol, behind. Ethanol is a colorless flammable liquid with a molecular formula of C2H6O, giving it a molar mass of 46.07 grams per mole. Ethanol is also characterized by a melting point of -114°C or 159 K.