Peroxidase Lab Report

The Effects on Peroxidase
Abstract: Enzymes are catalyst that speed up a chemical reaction that takes place inside a living cell. Enzymes speed up reactions by lowering the activation energy of a reaction. Peroxidase is an enzyme that is crucial in removing Hydrogen Peroxide which is produced as a by-product in some metabolic reactions. The lab was conducted to determine the effects of different factors on enzymes. Multiple experiments such as changes in temperature, concentration, inhibitors, Ph, and difference in the reaction rates were recorded. We divided the different experiments within our groups. Hydrogen Peroxide, Guaiacol, and peroxidase was prepared for the students, and each student used them accordingly for their experiments. Guaiacol

is a natural dye that was used as a measure for the reaction rate. It was realized that the peroxidase worked more efferently in 40 degrees Celsius.
Introduction: Peroxidase is an enzyme that is an essential part in expelling Hydrogen peroxide made in cells as consequence of some metabolic reactions.

Hydrogen peroxide is a harmful substance which could seriously harm cells if left to build up. Subsequently it is needed to discover the ideal conditions required for the enzyme to work accordingly. A progression of experiments were set up to test how temperature, ph, concentration and an inhibitor would influence the reaction rate of Peroxidase. Peroxidase is obtained by the concentrate from a turnip. The technique used to monitor the response amongst Peroxidase and hydrogen peroxide is by acquainting a color with the blend. "The color Guaiacol ties to Peroxidase and ends up plainly oxidized as the hydrogen peroxide is diminished to water." When Guaiacol is oxidized, it changes color to dark brown. A Spectrometer passes light through a substance which then records the absorbance of spectrums. The Dye changes the color according to the rate of the reaction. The Spectrometer records how fast the reaction happening. Utilizing this data, You could hypotheses about the trials. As concentration of Peroxidase increase, so will the reaction rate. If an inhibitor is acquainted with the Peroxidase arrangement, it will diminish the rate of response. Any change in temperature or pH will deform the enzyme and influence the response rate. In this way, the Enzyme will have an ideal pH and temperature where it works generally

effectively.
Materials and Methods:
The Temperature test was somewhat complex as each of the test tubes containing the arrangement must be changed with a specific temperature and recorded. The arrangements were mixed in five test tubes. The amount of Peroxidase was kept at 1 ml in all test tubes because the concentration was not being tested here. The tubes were them put in various holders of water that were at 0C, 23C, 40C, 60C, 100C. The Spectrophotometer was adjusted utilizing a "clear". The arrangements were moved into cuvettes and set in the Spectrophotometer. The outcomes were recorded for every temperature.