Wait a second!
More handpicked essays just for you.
More handpicked essays just for you.
Lab for enzyme activity
Lab for enzyme activity
Lab for enzyme activity
Don’t take our word for it - see why 10 million students trust us with their essay needs.
Purpose: This lab gives the idea about the enzyme. We will do two different experiments. Enzyme is a protein that made of strings of amino acids and it is helping to produce chemical reactions in the quickest way. In the first experiment, we are testing water, sucrose solution, salt solution, and hydrogen peroxide to see which can increase the bubbles. So we can understand that enzyme producing chemical reactions in the speed. In the second experiment, we are using temperature of room, boiling water, refrigerator, and freezer to see what will effect the enzyme.
Hypothesis: If a test tube filled with 3% hydrogen peroxide and catalase solution, the room temperature will increase the activity. Freezer, refrigerator, and boiling water will have
…show more content…
I blended on high to make the potatoes more liquid-like. I grabbed the cheesecloth and placed on the top of the blender. I poured the potato extract on the container and labeled it. I found out that I have to make 1% sugar solution so I grabbed the sugar and measured into 5 grams on the scale. I added 5 grams of sugar on 250 ml graduated cylinder and poured the water into the cylinder. I mixed the sugar with water and poured it into the saucepan. I refilled the water into the graduated cylinder and poured into the saucepan. I turned on the heat of the stove and saw the sugar dissolved. I poured into a container and labeled 1% sugar solution. I repeated the same thing with 1% salt solution by using 1 gram of salt and filled the water into graduated cylinder by 100 ml. I answered question three. In the first experiment, I grabbed four transfer pipets and used it to put solutions into the test tubes by 3ml. I labeled it and placed into the plastic cups so it can stand upright. I grabbed each test tube and poured 2 ml of catalase solution into it. I also tapped and swirled to measure the bubbles by using the ruler. I wrote the numbers into the lab report. In the second experiment, I labeled the room
In this experiment the enzyme peroxidase and the substrate hydrogen peroxide were not mixed initially, instead they were both placed in separate tubes and were incubated at a specific temperature, to prevent hydrogen peroxide from undergoing any reaction with peroxidase until they both acquire the required temperature.
Living organisms undergo chemical reactions with the help of unique proteins known as enzymes. Enzymes significantly assist in these processes by accelerating the rate of reaction in order to maintain life in the organism. Without enzymes, an organism would not be able to survive as long, because its chemical reactions would be too slow to prolong life. The properties and functions of enzymes during chemical reactions can help analyze the activity of the specific enzyme catalase, which can be found in bovine liver and yeast. Our hypothesis regarding enzyme activity is that the aspects of biology and environmental factors contribute to the different enzyme activities between bovine liver and yeast.
Catalase is a common enzyme that is produced in all living organisms. All living organisms are made up of cells and within the cells, enzymes function to increase the rate of chemical reactions. Enzymes function to create the same reactions using a lower amount of energy. The reactions of catalase play an important role to life, for example, it breaks down hydrogen peroxide into oxygen and water. Our group developed an experiment to test the rate of reaction of catalase in whole carrots and pinto beans with various concentrations of hydrogen peroxide. Almost all enzymes are proteins and proteins are made up of amino acids. The areas within an enzyme speed up the chemical reactions which are known as the active sites, and are also where the
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
Three flat-bottomed vials were obtained and labeled one through three. 3 mL of hydrogen peroxide was distributed to each of the vials. One drop of liquid soap was then added to each of the vials. The contents of the vials were gently swirled to ensure mixture of the hydrogen peroxide and soap. In each vial, a pH buffer was added; vial one received pH 2 buffer, vial two received pH 7 buffer, and vial three received pH 12 buffer. 1mL of catalase was then placed into each vial and the reaction was timed for 2 minutes. At the end of two minutes, the bubble column produced was measured and recorded into Table 3. The results were then graphed, as shown in Figure
According to the graph on amylase activity at various enzyme concentration (graph 1), the increase of enzyme dilution results in a slower decrease of amylose percentage. Looking at the graph, the amylose percentage decreases at a fast rate with the undiluted enzyme. However, the enzyme dilution with a concentration of 1:3 decreased at a slow rate over time. Additionally, the higher the enzyme dilution, the higher the amylose percentage. For example, in the graph it can be seen that the enzyme dilution with a 1:9 concentration increased over time. However, there is a drastic increase after four minutes, but this is most likely a result of the error that was encountered during the experiment. The undiluted enzyme and the enzyme dilution had a low amylose percentage because there was high enzyme activity. Also, there was an increase in amylose percentage with the enzyme dilution with a 1: 9 concentrations because there was low enzyme activity.
We then cut our potato tubes with the cork borer and cut them with the scalpel so they were the same length and weighed them. We then put one potato tube in each test tube and then added the same amount sugar solution in to each tube. The concentration of sugar solutions varied in each test tube.
Introduction / Background Information. This is an experiment to examine how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase. In this experiment I will be using yeast as a source of catalase. Enzymes are catalysts which speed up specific reactions. Enzymes such as catalase are protein molecules, which speed up a specific reaction within the cell.
middle of paper ... ... diction did work well because from my prediction and test I was able to match my ideas and theories with the experiment, so that part was a success, I did obtain anomalies as mentioned above and some improvements which could be made are more accurate measurements with weight and length of chips, make some changes with concentration levels of our sucrose solution, this has been mention previously on pages 9 and 10 and more time and care taken to improve on drying our potato chips; this my reduce the weight of our chips and give more accurate weight measurements when we do our final weigh in. I think was experiment was a good for obtaining evidence and therefore had no need to do a re-test of our potato chips, on a photocopied sheet of my graph I have circled my anomaly results (sucrose concentration level of 0.5M)
Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is important that a specific enzyme is present during the process. For example, lactase must be able to collaborate with lactose in order to break it down (Madar & Windelspecht, 105).
First of all, I started with the two cups to help provide the separation of the two potato slices and that helped by keeping the different mixed water solutions apart from one another by sitting in the cups. After that, I poured salt into one of the cups to help differentiate the effects in the potato by providing a solution into the water to help see an outcome in the slice. In order to put the right amount of salt into the cup, I also had to measure it in tablespoons to get the exact amount without going over the limited amount of salt needed in the experiment. With that being said, I then used a knife and tape measure to cut the precise size of the potato needed to conduct the experiment in order to fit in the cup. Not including the potato and water itself to explain the different solutions used and created as mixed in together.
pipette (1 ml) of a specific sugar and adding one mL of yeast to the sugar, mixing them together in a test tube with the letter that corresponds to the sugar according to the procedure. Collecting water at a temperature of thirty seven degrees celsius into a 250 ml beaker is the next step. Once the test tube and the 250 ml beaker is filled with the correct substances, the yeast and sugar in the test tube should be placed into the beaker and left for a ten minute incubation period. While waiting for the incubation period the LabQuest is calibrated according to the procedure provided. After the ten minute incubation period, the testing of carbon dioxide production will begin with collecting the mixture of yeast and sugar into a new, clean,
In this lab, it was determined how the rate of an enzyme-catalyzed reaction is affected by physical factors such as enzyme concentration, temperature, and substrate concentration affect. The question of what factors influence enzyme activity can be answered by the results of peroxidase activity and its relation to temperature and whether or not hydroxylamine causes a reaction change with enzyme activity. An enzyme is a protein produced by a living organism that serves as a biological catalyst. A catalyst is a substance that speeds up the rate of a chemical reaction and does so by lowering the activation energy of a reaction. With that energy reactants are brought together so that products can be formed.
Background information about terms used in the experiment. Enzymes are macromolecules that helps speed up the chemical reaction. Enzyme works by lowering the amount of energy thus, making the reaction easier to start. The substrate is the reactant molecule that is changed by the enzyme. And the compounds that are formed when a reaction goes to completion is called products. A pH, potential hydrogen is based on scale 0-14, where 7 is neutral, 0 being strong acid and 14 being strong base. pH is a measure of the concentration of hydrogen ions in solution. Catecholase is an enzyme which when catalyzed with catechol and oxygen forms a brown, product benzoquinone. Reaction rate is the