Essay On Catecholase

Our hypothesis is that we believe, the test tube that has potato extract alone in it will have the darkest brown color, thus it will have the highest absorbency. The reason we believe this hypothesis is true is because, darker the color of the mixture, more light will be absorbed and also because it did not receive any pH which can make the mixture lose it’s ability the catalyze reaction. This experiment is designed to investigate and compare, how the enzyme catecholase from potato extract reacts and shows different absorbency when mixed with catechol, and different pH buffer to when it just has the catecholse present in it. If our hypothesis is supported, then the tubes with the darkest color will have more absorbency.

Background information about terms used in the experiment. Enzymes are macromolecules that helps speed up the chemical reaction. Enzyme works by lowering the amount of energy thus, making the reaction easier to start. The substrate is the reactant molecule that is changed by the enzyme. And the compounds that are formed when a reaction goes to completion is called products. A pH, potential hydrogen is based on scale 0-14, where 7 is neutral, 0 being strong acid and 14 being strong base. pH is a measure of the concentration of hydrogen ions in solution. Catecholase is an enzyme which when catalyzed with catechol and oxygen forms a brown, product benzoquinone. Reaction rate is the

The dependent variable in our experiment was the reaction rate measured by absorbance in nanometers. Our control was a pH of 7. Standard variables in our experiment include the amount of substance used in the test tubes, for example, 3 mL of pH buffer in test tubes one through seven, 2 mL of catechol in test tubes one through seven, and 1 mL of potato extract in test tubes one through eight. To continue, same size test tubes, same size cuvettes, same size plastic pipets are also standard variables in our