How the concentration of enzymes controls the breakdown of Hydrogen Peroxide?
Aim-
We are investigating the effect of changing the concentration of the enzyme catalase has on the rate of breaking down of Hydrogen Peroxide.
Background Info-
This experiment will involve some soft potato. Potato has a very high enzyme count, that¡¦s why we use it in this experiment. This enzyme is called catalase. It is used in our body to break down food and other things that we might digest. A catalase speeds up the rate of reactions. The enzymes are all protean molecules. We will also use Hydrogen Peroxide and washing up liquid. The catalase breaks down the Hydrogen Peroxide into oxygen and water. The more vigorous the reaction the more froth that it creates.
The reaction in the experiment:
Hydrogen peroxide „³ oxygen + water
2H2O2 „³ O2 + H2O
The enzyme can be used around the body to help chemical reactions, it speeds them up! The enzyme breaks up larger molecules into smaller pieces. This is how the enzymes work below I have described some of the things that can affect how well it works.
Fair test/ Factors-
Temp- This can affect the movement of the particles. The higher the temp the faster the particles will move and the more collisions. If the temp of the Hydrogen Peroxide is too low the experiment will fail!
High Temp- It gives them more energy so they move faster this means that they are likely to connect the substrate and the active site. The high temp also gives the particles kinetic energy. This means that the Hydrogen Peroxide will be broken down quicker.
Best Temp- This is the hottest the enzyme can be heated without the active site changing (denaturing). If the active site changes then the reaction will no longer work. From our preliminary test we found out that the optimum temp is 25oc this is around room temp.
PH- All enzymes have an optimum PH, the wrong PH can cause denaturing. The Ph can¡¦t be too acid or alkaline so keep it as near to 7 as possible. If the enzyme denatures then it is no use for breaking down the Hydrogen Peroxide.
Concentration of H2O2- The higher the concentration the more collisions with the active site. This means there is an increased rate of reaction. Creating more froth so the concentration will have to be the same for each of the test tubes.
The results of this experiment showed a specific pattern. As the temperature increased, the absorbance recorded by the spectrophotometer increased indicating that the activity of peroxidase enzyme has increased.At 4C the absorbance was low indicating a low peroxidase activity or reaction rate. At 23C the absorbance increased indicating an increase in peroxidase activity. At 32C the absorbance reached its maximum indicating that peroxidase activity reached its highest value and so 32 C could be considered as the optimum temperature of peroxidase enzyme. Yet as the temperature increased up to 60C, the absorbance decreased greatly indicating that peroxidase activity has decreased. This happened because at low temperature such as 4 C the kinetic energy of both enzyme and substrate molecules was low so they moved very slowly, collided less frequently and formed less enzyme-substrate complexes and so little or no products. Yet, at 23 C, as the temperature increased, enzyme and substrate molecules
For example, substrate concentration, enzyme concentration, and temperature could all be factors that affected the chemical reactions in our experiment. The concentration of substrate, in this case, would not have an affect on how the bovine liver catalase and the yeast would react. The reason why is because in both instances, the substrate (hydrogen peroxide) concentration was 1.5%. Therefore, the hydrogen peroxide would saturate the enzyme and produce the maximum rate of the chemical reaction. The other factor that could affect the rate of reaction is enzyme concentration. Evidently, higher concentrations of catalase in the bovine liver produced faster reactions, and the opposite occurs for lower concentrations of catalase. More enzymes in the catalase solution would collide with the hydrogen peroxide substrate. However, the yeast would react slower than the 400 U/mL solution, but faster than the 40 U/mL. Based on this evidence, I would conclude that the yeast has a higher enzyme concentration than 40 U/mL, but lower than 400
Catalase is a common enzyme that is produced in all living organisms. All living organisms are made up of cells and within the cells, enzymes function to increase the rate of chemical reactions. Enzymes function to create the same reactions using a lower amount of energy. The reactions of catalase play an important role to life, for example, it breaks down hydrogen peroxide into oxygen and water. Our group developed an experiment to test the rate of reaction of catalase in whole carrots and pinto beans with various concentrations of hydrogen peroxide. Almost all enzymes are proteins and proteins are made up of amino acids. The areas within an enzyme speed up the chemical reactions which are known as the active sites, and are also where the
This would give us an extra measure of accuracy each time. Another way to improve the experiment and to produce consistent readings was to used distilled water. This is because the distilled water contains no impurities and therefore no hardness in water.
This means that there are more collisions between the solid and liquid. Amount of tap water H2O used, i.e. volume (in ml) of the syringe. Amount of Alka-Seltzer tablets used, i.e. quantity of the tablets. one is used for the. Number of times the solution is stirred.
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
With this information we were able to identify any patterns and similarities. Hypothesis: The higher the temperature of water, potato and H²O², the rate at which the Enzyme will work will be faster therefore producing more oxygen. The reaction will be the same without the catalase (potato). Therefore in both experiments the Enzyme will work more rapidly and produce more oxygen. Aim: To test the hypothesis.
As the levels of H2O2 increase, gas is produced faster and in a greater quantity. This means that the height of the froth will increase. The froth is produced due to the gas as it bubbles from the potato slice. This gas is given off as a waste product from the reaction where H2O2 (substrate) breaks down the enzymes in the potato. If I were to roughly plot a graph for the reaction, it would look like this, [IMAGE] The graph shows that as the concentration of hydrogen peroxide is increased the more oxygen is given off.
“The number of people receiving methadone maintenance treatment in Ontario has increased sharply in recent years, from 6,000 in 2000 to 38,000 in 2012” (CAMH). Though this number seems threatening, it is a sign that Canada’s effective methadone treatments are reaching more people. Methadone is a synthetic opioid; it has a different chemical structure but similar effects on the body to other opiates such as morphine, heroin, codeine and OxyContin (Harvard Health Publications). Methadone is not used to cure, but rather treat addictions that individuals have to more dangerous opioids. Critics of the maintenance treatment say these rising numbers is proof of addicts getting high with Canadian’s tax money. A more accurate statement would be that
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In this lab, it was determined how the rate of an enzyme-catalyzed reaction is affected by physical factors such as enzyme concentration, temperature, and substrate concentration affect. The question of what factors influence enzyme activity can be answered by the results of peroxidase activity and its relation to temperature and whether or not hydroxylamine causes a reaction change with enzyme activity. An enzyme is a protein produced by a living organism that serves as a biological catalyst. A catalyst is a substance that speeds up the rate of a chemical reaction and does so by lowering the activation energy of a reaction. With that energy reactants are brought together so that products can be formed.
Because there are more molecules, the frequency of successful collisions is greater, and the reactions happen faster. Safety There are a lot of safety issues we must abide by in this experiment. We must remember that the substances we use in this experiment can be very harmful if used the wrong way. When doing this experiment, it may be necessary to wear safety goggles, as things are very unpredictable, and even though it is very unlikely that the solution will come out of the beaker during the experiment, one must still be cautious of spills. We must make sure that coats and bags are all out of the way while doing the experiment.
During the world cup mass protests took place to expose how the country invested so much in preparing for the World Cup while so many residents were living in poverty (Human Rights Watch, 2014). This issue has the potential to become volatile as the country is currently experience an economic slowdown and the unemployment numbers begin to rise. Another result of extreme poverty is the lack of skilled workforce which makes it difficult for companies staff their business with local residents which keeps them from investing in the community (Deloitte,