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Enzymes and their functions
How changing the concentration on the substrate affects the enzyme activity
How the surface area of a substance influence the rate of reactions
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Recommended: Enzymes and their functions
The Effect of Changing the Concentration of the Enzyme Catalyst on the Rate of Reaction on Hydrogen Peroxide
Planning
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Hydrogen Peroxide Water + Oxygen
2H2O2 2H2O + O2
Introduction - Background Information
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To help me understand what a chemical reaction is or involves I did
some research.
A chemical reaction is a process by which atoms or groups of atoms are
redistributed, resulting in a change in the molecular composition of
substances.
Enzymes are a biological catalyst, which controls a cellular reaction,
they are proteins that act as a catalyst. A catalyst is a substance
that speeds up a reaction but does not get used up. It works by
reducing the Activation Energy, which is the minimum energy needed for
a reaction to happen. A catalyst can make a reaction occur even if it
would not happen other wise. Enzymes only affect the speed at which a
product is formed, not how much is produced. Enzymes are specific,
this means that once an enzyme has acted on one substance it will not
act on a different one.
There are two reactions which involve enzymes they are anabolic and
catabolic. An anabolic reaction is a build up of smaller molecules
into larger molecules. A catabolic reaction is the speeding up of the
reaction time but is the breaking down of a substance. I will be
focusing on the catabolic reaction. To explain the reaction there is a
method called the lock and key theory:
The Collision Theory - this is when reacting molecules collide with
each other with enough energy to react. Surface area, temperature,
concentration and the use of an enzyme affect the collision theory.
All of these factors increase the rate of reaction if you increase
them. If there is a larger surface area then the molecules are exposed
and are more available to react as there are more of them. This means
there will be more collisions so a faster reaction.
If you increase the temperature this will make the molecules move more
Investigation of How the Concentration of Catalase Enzyme Affects the Rate of Reaction Aim: To find out how the concentration of Catalase Enzyme will affect the enzyme activity and the rate of reaction towards Hydrogen Peroxide. (H O ) Prediction: I predict that with the higher concentration of enzyme, the likelihood of it breaking down molecules will be greater because there will be more enzymes to work at the substrate and the chances of it colliding will be higher making the activity time quicker. Equipment: · Syringe · Measuring Cylinder (×2) · Knife · Blender · Beakers (×2) · Balance · Hydrochloric Acid in a beaker · Stop clock · Potato · Water in a beaker Preliminary Experiment: In this experiment we will be using an enzyme called Catalase. By using different amounts of this enzyme we will be diluting it with water to test how the concentration of Catalase affects the rate of reaction with Hydrogen Peroxide.
Enzymes are biomolecules that catalyze or assist chemical reactions. ("Enzyme Information - Disabled World", n.d.,) Without enzymes it would be impossible for an organism to carry out chemical reactions. Enzymes are proteins that carry a chemical reaction for a specific substance or nutrient. For example, the digestive enzymes help food to be broken down so it can be absorbed. Enzymes can either initiate the reaction or speed it up. Substrates are the chemicals that are transformed by enzymes. (Gunsch & Foster, 2014) Reactants are the chemicals in the absence of enzymes. Metabolic pathways that occur in a cell are determined by a set of enzymes which are selective for their substrates and catalyze only a few reactions among the many possibilities.
It is important however to note that the NH4 and K ions are still in
Background information:. Enzyme Enzymes are protein molecules that act as the biological catalysts. A Catalyst is a molecule which can speed up chemical reactions but remains unchanged at the end of the reaction. Enzymes catalyze most of the metabolic reactions that take place within a living organism. They speed up the metabolic reactions by lowering the amount of energy.
Enzymes are biological catalysts. They speed up the rate of a reaction without themselves being used up. Enzymes are individual proteins which controls a particular reaction or type of reaction. They are specific e.g. amylase always acts on starch.
This enzyme speeds up the break down of hydrogen peroxide into water and oxygen, as enzymes are biological catalysts. [IMAGE]The reaction: Hydrogen peroxide Water + Oxygen Catalase -------- [IMAGE] 2H2O2 2H2O + O2 Apparatus: Hydrogen Peroxide, Several sticks of celery, Stand, boss and clamp, 100ml conical flask, 25cm3 burette, 1800cm3 beaker, Rubber bung with delivery tube, Distilled water, Large container filled with water, 10cm3 measuring cylinder, 10cm3 syringe, 20cm3 syringe, Blender, Knife, Ceramic tile, Electronic balance (correct to 2 decimal places), Sieve, Stopwatch/timer. The variables: There are many possible variables in this investigation, such as pH, temperature, the concentration of substrate and the concentration of the enzyme.
I shall be measuring how much gas is given off. This will be done by measuring the amount of froth on the surface of the liquid. The oxygen released is collected in the form of these bubbles. The equation for the reaction is: (catalase) [IMAGE] H2O2 2H2O + O2 (hydrogen peroxide) (2 part water) (oxygen) I will change the concentration of H2O2 and O2 (making sure the volume stay the same, when one part of a H2O2 particle is taken, an O2 particle is added. Prediction
The Effect of Concentration on Reaction Rate Introduction I will be carrying out an investigation into how concentration affects reaction rate. I will be looking at sodium thiosulphate and hydrochloric acid. The reaction is represented by the following equation: [IMAGE]Na2S2O3(aq) + 2HCl(aq) 2NaCl(aq) + H2O(l) + SO2 (g) + S (s) I will add the hydrochloric acid to the Sodium Thiosulphate and time how log it takes for the mixture to turn opaque. I will use a different concentration of Sodium Thiosulphate each time. Prediction
Enzymes have the ability to act on a small group of chemically similar substances. Enzymes are very specific, in the sense that each enzyme is limited to interact with only one set of reactants; the reactants are referred to as substrates. Substrates of an enzyme are the chemicals altered by enzyme-catalysed reactions. The extreme specific nature of enzymes are because of the complicated three-dimensional shape, which is due to the particular way the amino acid chain of proteins folds.
To begin our discussion of enzyme kinetics, let's define the number of moles of product (P) formed per time as V. The variable, V, is also referred to as the rate of catalysis of an enzyme. For different enzymes, V varies with the concentration of the substrate, S. At low S, V is linearly proportional to S, but when S is high relative to the amount of total enzyme, V is independent of S. Concentrations is important in determining the initial rate of an enzyme-catalyzed reaction. A more thorough explanation of enzyme rates can be found here: Definition of Reaction Rate.
There are five factors which affect the rate of a reaction, according to the collision theory of reacting particles: temperature, concentration (of solution), pressure (in gases), surface area (of solid reactants), and catalysts. I have chosen to investigate the effect of concentration on the rate of reaction. This is because it is the most practical way to investigate. Dealing with temperatures is a difficult task, especially when we have to keep constant high temperatures. Secondly, the rate equation and the constant k changes when the temperature of the reaction changes.
put it in a conical flask. I will put the flask on top of a piece of
which will be easier to time and we can measure the size of the froth
Chemical kinetics is the study and examination of chemical reactions regarding re-arrangement of atoms, reaction rates, effect of various variables, and more. Chemical reaction rates, are the rates of change in amounts or concentrations of either products or reactants. Concentration of solutions, surface area, catalysts, temperature and the nature of reactants are all factors that can influence a rate of reaction. Increasing the concentration of a solution allows the rate of reaction to increase because highly concentrated solutions have more molecules and as a result the molecules collide faster. Surface area also affects a
The first experiments investigate the order of reaction with respect to the reactants; hydrogen peroxide, potassium iodide and sulphuric acid by varying the concentrations and plotting them against 1/time. An initial rate technique is used in this experiment so ‘the rate of reaction is inversely proportional to time.’ To find the order of reaction in respect to the reactants, 1/time is plotted against the concentration of Hydrogen Peroxide using the equation: