The three deeps above show motility test. My results are right: very motile bacteria, center: Non-motile bacteria, left: Motile bacteria. The type of instrument used to inoculate a deep is a needle.
Type of media below is right: Slant, center: Broth, and left: Deep. The difference in the media types are slant and deep are solid and contains agar. However, broth is liquid. The instrument I used to inoculate slant : loop, broth: needle or loop, and deep: needle.
I can do to verify its purity by viewing colonial morphology. I will streaking this culture on the plate by using streak plate method and viewing their cellular morphology by preparing a smear. I also view it by using a microscope which it will be 1000x. If the culture was not pure, I would notice the streak plate may have colonies with different colonial morphology, color, and cells. I have to view it in the microscope so it may have different cellular morphology and staining properties. The five ways I can contaminate a culture during inoculation are accidentally touching the loop or needle, not carefully holding the broth and leave it on the table so it will leaking down the tube, working in the lab that have a lot of airborne contaminants which it is mixtures exposure by absorption through skin, a sample open for a long time, and forget to flame the needle, loop, or tube.
…show more content…
To avoid it, you need to be careful to handle TSA. Do not open the lid, touching it on the surface, and passing down. You need to make sure to follow the steps and keep the area clean and
I identified the genus and species of an unknown bacterial culture, #16, and I applied the following knowledge of morphologic, cultural and metabolic characteristics of the unknown microorganism according to the laboratory manual as well as my class notes and power point print outs. I was given an incubated agar slant labeled #16 and a rack of different tests to either examine or perform myself; the tests are as follows: Gram Stain; Nutrient Gelatin Test; Carbohydrate Fermentation; Dextrose, Lactose and Sucrose; IMVIC tests; Citrate, Indole, Mythel-Red and Vogues Proskauer test; as well as a Urease and TSI Test. Materials and Methods/Results Upon receiving the Microorganism (M.O.) #16, I prepared a slide by cleaning and drying it. Then, using a bottle of water I placed a sterile drop of water on the slide and used an inoculating loop, flame sterilized, I took a small sample of the unknown growth in my agar slant and smeared it onto the slide in a dime sized circle and then heat fixed it for ten minutes.
Prior to intubation for a surgical procedure, the anesthesiologist administered a single dose of the neuromuscular blocking agent, succinylcholine, to a 23-year-old female to provide muscular relaxation during surgery and to facilitate the insertion of the endotracheal tube. Following this, the inhalation anesthetic was administered and the surgical procedure completed.
After 48 hours of incubation the agar plates were viewed. Individual colonies were tested for successful isolation by gram staining and then viewing the stained bacteria under a microscope. Isolation was successful. One colony of each unknown bacteria was transferred to an agar slant for growth. The agar slants were stored at room temperature over the weekend so that they would not grow too much.
Streak plate technique was used to isolate pure culture for each bacteria (2). The Gram stain was used to determine Gram reaction and morphology of each bacteria (2) Selective and differential media such as, salt agar, MacConkey agar and blood agar were used for bacterial identification (2). Gelatin deeps were inoculated to detect production of gelatinase (2). Starch Agar plate were inoculated to detect amylase (2). Ocular reticle used to determine bacteria size (2). Motility deeps were inoculated to detect motility on bacteria (2). Thioglycollate broth used to determine oxygen requirements (2). Carbohydrate fermentation
I want to give Dr. Oster the round of applause because I had that same experience every time I went through TSA scanners whenever I flew out to different states for ballroom dancing competitions. I always hollered, “Hey, mister (or ma’am), please be informed that I wear a hearing aid and it may accidentally set that alarm off.” These TSA agents really did look nervous every time. As always, they got me through in a split second. LOL! Of course, last time, I flew was like three years ago since I am too busy doing the writing assignments for the doctoral program.
They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species.
I also inoculated a tryptic soy broth (TSB), a nutrient gelatin deep, a motility agar deep, a fluid thioglycollate medium (FTM) tube, and a TSA plate with my unknown culture. All of these inoculated media were incubated until the next class period (about 48 hours). Then when I came to class most of my inoculated tubes and my streak plate appeared to have growth. The next step I took was making a gram stain to determine the gram reaction and cellular morphology of my unknown. I used my working slant to do this, after careful examination of the gram stain, I learned that my unknown was a gram-positive bacterium. I then preceded by making a negative stain to see the size of the cells of my unknown bacteria. The cell shape was cocci and the cells occurred in clusters of tetrads. After discovering that my unknown bacteria was gram-positive cocci, I turned to page 207 of the lab manual to narrow down my options, there was only four out of the gram-positive list that were
One of the first new pieces of technology the TSA introduced were better screeners. A new screener known as AIT or Advance imaging technology has been created to counteract items that may have been hidden from site or...
Since 9/11 there have been nine major security changes at airports. The most important changes are the liquid ban, which bans liquids more than 3.4 ounces from being on an airplane and the body-scan machines along with the enhanced pat downs. (Seaney 1)l/ The body scan machines are x-ray machines that scan the person for anything that could be used as a weapon. Enhanced pat downs are violating to some people as they do not like their private areas searched for weapons. But these pat downs are necessary to detect, find, and remove weapons that a TSA (Transportation Security Administration) agent cannot see with their own eyes. The last important change worth mentioning is that all special items including laptops, tablets, E-readers, and all electronic items have to be checked for explosives and other threats. These changes as well as other minor regulations for passengers give the TSA an extra line of defense while screening air travelers.
The purpose of experiments 1 and 3 were to determine the relationship between the intensity of EMG activity of a muscle contraction in the dominant and non-dominant arm of the subject. As the consecutive squeezes were stronger, the both the absolute integral of EMG activity and muscle force increases. This is because there is a linear relationship between the absolute integral of force and EMG, however, this is only true when the muscles are activated isometerically (true for this experiment). There are many types of contractions, two main ones are: when the muscle length changes (isotonic), and when it stays the same (isometric). The force produced by muscles depend on its length, velocity of movement and frequency, and when a contraction
Thus, there is evidence to demonstrate that the change in voltage across the membrane of cells are subsidised by the type of food and the bite size. Additionally, the measureable mV differences amongst relaxed and contracted muscles are on mutual wavelength.
the gain or loss of water when samples of the tissue are placed in a
stains on sputum’s and body fluids, and have completed a few AFB cultures. Apart from
material for gel entrapment methods. Cells might either be allowed to seep deep inside or the