Identification Process Lab Report

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Introduction
The purpose of this experiment is to appreciate the identification process and recognize its importance in various settings such as clinical, research and industrial. The identification of unknown microbes may be critical when developing an effective treatment method. For example, when an individual is ill and the physician must treat them, they must first identify the microbe infecting the patient in order to efficiently prescribe the correct treatment. Without following scientific processes to identify the microbe physicians are left with a less effective, trial-and-error treatment plan1. This will cause the prescribed method of treatment to only be ‘guess’ conduct1. Another reason for the requirement of identification process …show more content…

“The analysis of food for the presence of both pathogenic and spoilage bacteria is a standard practice for ensuring food safety and quality.2” One method used in order to detect these types of microbes is called the conventional method. It is a very inexpensive, and sensitive method that can give numeric and quality data on the amount and nature of the microorganisms existing in the food sample. Although this method is very inexpensive it does require several days before being able to see the colonies. Not only is it a time sensitive procedure but it is also labor intensive consisting of, “culture medium preparation, inoculation of plates and colony counting.2” Though this is a beneficial method, it is very time sensitive. Additional prompt methods are needed in the food industry in order for constructiveness. In exercise 14, the identification process of the unknown microbes, four different techniques were used in order to identify the unknown microbes. The first crucial step in the procedure was finding pure culture. The microbes must produce separate colonies in order to use them in the next step known as gram staining. The gram stain procedure classified whether the microbes were gram positive or gram negative. The microbes developed unique …show more content…

The purpose to identify the two microbes by using numerous different techniques was accomplished. Not only were the microbes identified but the appreciation for the identification process has grown. The crucial role identification process plays in society is more apparent and fundamental than it appears. Identifying bacteria can be crucial with the well-being of humans and their consumption. Not only their well-being but identifying bacteria is a type of preventative measure to stop death or illnesses before the bacteria spreads. After gram staining it appeared that both microbes were purple therefore both gram negative. These results gave off the illusion that both unknown bacteria were able to retain the primary stain. This was an error due to over staining that occurred in the process of gram staining specifically with microbe B. Luckily, the two were distinguishable after the Lactose test results were presented. This is because lactose tests for the production of acid and gas. The results for microbe A were negative meaning acid production did not occur with this unknown microbe and therefore had to be B. sub. On the other hand, microbe B resulted in the assembly of both gas and acid production. Thanks to this result it was apparent that the unknown microbe B could not be gram positive. Gram negative organisms do not produce bubbles when tested with Lactose broth. This gave the indication that

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