Discussion: This discussion was aimed to observe and measure DNA molecules. Because of negative charge, DNA migrates towards the positive electrode (anode). Hence, the cathode must be placed on the side close to the contained sample wells, whereas the anode placed on the opposite position. And approximate of 100V is provided to the system, DNA molecules keep migrating until the dyes reach the end of the gel. After electrophoresis, use Ethidium Bromide (C21H20BrN3), which links with DNA molecules and fluoresces under ultraviolet (UV) light to observe the DNA fragments on the gel. Photographing the lit gel under ultraviolet light in a dark room to record the result. Movement speed of DNA in the electric field depends on many factors such as electric power, buffer composition, concentration agarose gels. The higher concentrations of gel, the stronger resistance, which leads to DNA move slower. Moreover, the size of the DNA molecules also affects the speed of their movement in the gel. For example, in the same conditions of the electric field, concentration of agarose and buffer, moving speed between two different DNA molecules base on their sizes, the smaller molecules move faster than bigger ones. Since …show more content…
This technique is especially useful in splitting biological molecules such as DNA (deoxyribonucleic acid), RNA (ribonucleic acid). It is to detect DNA sequences, analyze mixtures of DNA sequences. Electrophoresis technique, in fact, can be used to determine the full size of the bacterial chromosomes, or chromosomes of eukaryotes, such as yeast. These species have the size of a few Mb genome. It also used to analyze of PCR products e.g. in diagnosing genetic molecular. This technique allows the researchers can quickly diagnose diseases before and after childbirth, Anemia, Cystic Fibrosis, Huntington and find new treatments for genetic
The unknown bacterium that was handed out by the professor labeled “E19” was an irregular and raised shaped bacteria with a smooth texture and it had a white creamy color. The slant growth pattern was filiform and there was a turbid growth in the broth. After all the tests were complete and the results were compared the unknown bacterium was defined as Shigella sonnei. The results that narrowed it down the most were the gram stain, the lactose fermentation test, the citrate utilization test and the indole test. The results for each of the tests performed are listed in Table 1.1 below.
The two modes of analysis that will be used to identify an unknown insert piece of DNA would be plating the transformation cells onto LA plates that have either ampicillin or chloramphenicol and PCR. We will use the PCR thermocycler to denature the restriction enzymes that were specifically used to assimilate the vector DNA. It is important to use the PCR thermocycler because denaturation of the restriction enzyme will prevent the restriction enzyme from cutting the vector DNA, after the insert DNA has assimilated to the vector DNA. After the addition of specific primers that complement the base pair to its corresponding target strand, PCR will be used. Subsequently, Taq polymerase will be used to determine whether the insert DNA has been properly assimilated to the vector DNA. Within this specific situation, the target strand will be the insert DNA. After we let the PCR thermocycler run for approximately 2 ½ hours, we will then put our PCR products in the gel and run the gel to completion. After the gel has run to completion, we will then take a photograph of the gel using the UV transilluminator with the assistance of our TA. If the insert DNA was properly assimilated to the vector DNA, then our corresponding gel photo would have one band. After the cells have been transformed, we would g...
Living organisms undergo chemical reactions with the help of unique proteins known as enzymes. Enzymes significantly assist in these processes by accelerating the rate of reaction in order to maintain life in the organism. Without enzymes, an organism would not be able to survive as long, because its chemical reactions would be too slow to prolong life. The properties and functions of enzymes during chemical reactions can help analyze the activity of the specific enzyme catalase, which can be found in bovine liver and yeast. Our hypothesis regarding enzyme activity is that the aspects of biology and environmental factors contribute to the different enzyme activities between bovine liver and yeast.
The affects of pH, temperature, and salt concentration on the enzyme lactase were all expected to have an effect on enzymatic activity, compared to an untreated 25oC control. The reactions incubated at 37oC were hypothesized to increase the enzymatic activity, because it is normal human body temperature. This hypothesis was supported by the results. The reaction incubated to 60oC was expected to decrease the enzymatic activity, because it is much higher than normal body temperature, however this hypothesis was not supported. When incubated to 0oC, the reaction rate was hypothesized to decrease, and according to the results the hypothesis was supported. Both in low and high pH, the reaction rate was hypothesized to decrease, which was also supported by the results. Lastly, the reaction rate was hypothesized to decrease in a higher salt concentration, which was also supported by the results.
Upon completion of the experiment we were able to examine the DNA. First, the electrophorese
The given DNA ladder sample and each individual ligation samples were run on 40ml of 0.8% agarose in 1x TAE buffer for approximately sixty minutes at 110V. The appropriate volume of 6x GelRed track dye was used after it was diluted to a final concentration of 1x and incubated for thirty minutes. Finally, the gel was illuminated under UV light and analyzed.
As the solution pH can influence the stability of NaClO-NH3 blend and the elimination of SO2, NOx, the impact of the pH of NaClO-NH3 blend solution on the instantaneous removal as well as the duration time was investigated, and the final pH after reaction was also detected and shown in Fig. 5. It can be seen that the variation of solution pH has a negligible effect on the desulfurization, but the elevated pH has a great promotion on the NOx removal, the efficiencies are significantly increased from 36% to 99% for NO2 in the pH range of 5–12 and from 19% to 65% for NO when the pH is between 5 and 10, after where, both of them are constant. Hence, the optimal pH of the NaClO-NH3 solution for the
In biology class, we were learning about enzymes. Enzymes are proteins that help catalyze chemical reactions in our bodies. In the lab, we were testing the relationship between the enzyme catalase and the rate of a chemical reaction. We predicted that if there was a higher percentage of enzyme concentration, then the rate of chemical reaction would increase or it would take less time. We placed 1 ml of hydrogen peroxide into four depressions. Underneath the first depression, we place 1 ml of 100% catalase and make 50% dilution with 0.5 ml of water. We take 50% of that solution and dilute with 0.5 ml of water and we repeat it two more times. there were four depressions filled with catalase: 100%, 50%, 25% , 12.5 % with the last three diluted
Deoxyribo Nucleic Acid (DNA) is a chromosome found in the nucleus of a cell, which is a double-stranded helix (similar to a twisted ladder). DNA is made up of four bases called adenine (A), thymine (T), guanine (G), and cytosine (C), that is always based in pairs of A with T and G with C. The four bases of A, C, G, and T were discovered by Phoebus Levene in 1929, which linked it to the string of nucleotide units through phosphate-sugar-base (groups). As mention in Ananya Mandal research paper, Levene thought the chain connection with the bases is repeated in a fix order that make up the DNA molecu...
DNA analysis is a scientific process among the newest and most sophisicated of techniques used to test for genetic disorders, which involves direct examination of the DNA molecule itself (Lyman, 2014) . Today crime labs use mtDNA analysis. This type of analysis allows smaller degraded pieces of DNA to still be successfully tested (Lyman, 2014) . There are several steps taken when analyzing DNA in forensics. When testing scientists must first isolate the DNA so it is not contaminated and can't be used. Lab technicians the take small pieces of the DNA, conserving as much as they can encase they need to test again. Once testing is done the next step is determining the DNA test results and finally there is the comparison and interpretation of the test results from the unknown and known samples to determ...
Tsou, J. A., Hagen, J. A., Carpenter, C. L., & Laird-Offringa, I. A. (2002, August 05). DNA
Gel electrophoresis is used in a variety of settings, particularly in molecular biology. Besides being used to separate nucleic acids, such as DNA and RNA, gel electrophoresis is also employed to divide proteins (Gel Electrophoresis). According to research, electrophoresis is applied for the following reasons, "To get a DNA fingerprint for forensic pur...
LAB REPORT 1st Experiment done in class Introduction: Agarose gel electrophoresis separates molecules by their size, shape, and charge. Biomolecules such as DNA, RNA and proteins, are some examples. Buffered samples such as glycerol and glucose are loaded into a gel. An electrical current is placed across the gel.
The scientific and medical progress of DNA as been emense, from involving the identification of our genes that trigger major diseases or the creation and manufacture of drugs to treat these diseases. DNA has many significant uses to society, health and culture of today. One important area of DNA research is that used for genetic and medical research. Our abi...
This causes the DNA fragments to move through the gel depending on their sizes. With this, the DNA fragments will show a sample that will determine how large they are to one another. Gel electrophoresis uses a horizontal gel-like slab. These gels are made of polysaccharide called agarose, which is dry, powdered flakes. When the agarose is heated in a buffer, it makes the gel form solid, slightly squishy gels. (Dickey, J. L. 2012) At one end of the gel, there is square shaped space that is called wells, this is where the DNA sample will be placed. Before the DNA samples are added, the gel must be added to the well of the gel box. One end of the box is hooked to a positive electrode and the other a negative electrode. When active, the DNA samples will start to migrate from the positive to the negative showing a difference with the DNA samples with some fragments that are larger and some that are smaller. (Mader. S. S. 2016)