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Essay on food safery standards
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TITLE OF THE PROPOSAL
Does human preparation of salads cause food poisoning?
JUSTIFICATION OF THE STUDY
The association of food and microorganisms were developed long before beginning of recorded history. Foods are not only classed as nutritious to consumers, but are also play an excellent source for microbial growth. The spoilage or contamination of food all depends on the microorganisms. Food poisoning is an illness that is caused due to the consumption og eating food that is contaminated. The food standard agency has estimated that in UK each year 5.5 million people get affected by food poisoning (NHS, 2013). Ready to eat food are defined as foods that are produced by the manufacturer for direct human consumption with exclusion of any need for cooking or any processing. In the UK, salad consumption has increased widely; salads are considered as safe by the consumers. In recent years it has increased both in quantity and variety. Due to the governments 5- A- Day campaign, the trend in ready to eat salads has increased widely in UK (Little et al, 2008). Salads have become the main meal favourite of people nowadays, with the addition and accomplishment of cheese, pasta, couscous etc. (Food safety agency, 2014)
This study is based on testing of refrigerated ready to eat salads which includes both fresh cut and bagged salads. In this study the main bacterium that is going to be determined are Listeria spp. Specifically Listeria monocytogenes. The study is aimed to establish the microbiological quality of ready to eat fresh cut salads and bagged salads from different catering and retail premises.
RESEARCH BACKGROUND
The common causes of food poisoning are bacteria and viruses. Depending on the bacteria or viruses that contaminate ...
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...ring this week, I will be collecting results from the second week and will be making my second enrichment culture.
Fourth week : During this week, I will be collecting results from the third week and plating the colonies onto different selective media (Oxford and PALCAM agar)
Fifth week: During this week, I will be collecting results from fourth week and will restreak the colonies into a non selective medium ( Tryptic soy agar)
Sixth week: during this week, I will be collecting results from fifth week and conducting different biochemical testing which include gram stain, catalase, acid production of carbohyrdrate test and API Listeria.
Seventh week: This week will be used for the collection of the biochemical testing from the sixth week.
Eighth week : this week will be used for the data analysis of the results. This will be done with the help of the SPSS software.
I identified the genus and species of an unknown bacterial culture, #16, and I applied the following knowledge of morphologic, cultural and metabolic characteristics of the unknown microorganism according to the laboratory manual as well as my class notes and power point print outs. I was given an incubated agar slant labeled #16 and a rack of different tests to either examine or perform myself; the tests are as follows: Gram Stain; Nutrient Gelatin Test; Carbohydrate Fermentation; Dextrose, Lactose and Sucrose; IMVIC tests; Citrate, Indole, Mythel-Red and Vogues Proskauer test; as well as a Urease and TSI Test.
After 48 hours of incubation the agar plates were viewed. Individual colonies were tested for successful isolation by gram staining and then viewing the stained bacteria under a microscope. Isolation was successful. One colony of each unknown bacteria was transferred to an agar slant for growth. The agar slants were stored at room temperature over the weekend so that they would not grow too much.
I also inoculated a tryptic soy broth (TSB), a nutrient gelatin deep, a motility agar deep, a fluid thioglycollate medium (FTM) tube, and a TSA plate with my unknown culture. All of these inoculated media were incubated until the next class period (about 48 hours). Then when I came to class most of my inoculated tubes and my streak plate appeared to have growth. The next step I took was making a gram stain to determine the gram reaction and cellular morphology of my unknown. I used my working slant to do this, after careful examination of the gram stain, I learned that my unknown was a gram-positive bacterium. I then preceded by making a negative stain to see the size of the cells of my unknown bacteria. The cell shape was cocci and the cells occurred in clusters of tetrads. After discovering that my unknown bacteria was gram-positive cocci, I turned to page 207 of the lab manual to narrow down my options, there was only four out of the gram-positive list that were
Salmonella is one danger that has caused many effects to consumers. Walsh writes about one incident when an outbreak “from tainted peanuts that killed at least eight people and sickened 600,” (Walsh 167). This incident left many people asking the same question, how can we trust the food that we put into our bodies? Salmonella, a type of food poisoning caused by bacteria found on different food types has caused an epidemic because of its domino effect on food and our health. Once one factory is contaminated, that factory could be housing both crops and meat, which is then transferred to our supermarkets and on our dinner tables. ...
When the PCR technique is completed, the tubes are stored at 4°C until analysis of the tubes. To analyze the PCR results with the gel electrophorese, 2.5ul of the 10x loading dye is added to each PCR reaction tube. The gel for the electrophorese consists of 1.5% agarose gel with 0.5x TBE and 200ng/ml ethidium. bromide. The sand is a sand.
A streak for isolation is made on the plate. In addition, check plate for hemolysis. Look for the organism’s ability to produce hemolysin. Bacteria with hemolysin will generate different patterns. To start with, partially green around the colony is alpha. Secondly, complete clearing around the colony is betta. Thirdly, cloudiness or no result is gamma. The controls are K. pneumoniae, B. cereus, and S. saprophyticus in order based on previous results.
Salmonella is one of the most common causes of food poisoning. It is a gram-negative, aerobic (oxygen requiring), rod-shaped bacterium that can infect humans, birds, reptiles, and other animals. It results in the swelling of the lining of the stomach and intestines. Salmonella food poisoning occurs worldwide, however it is most frequently reported in North America and Europe. In the United States, Salmonella is responsible for about 15% of all cases of food poisoning (Salmonella food poisoning).
Leboffe, M. J., & Pierce, B. E. (2010). Microbiology: Laboratory Theory and Application, Third Edition 3rd Edition (3rd Ed.). Morton Publishing
What is Foodborne Illness? According to a medical dictionary, foodborne illness is an acute gastrointestinal infection caused by consuming food contaminated with pathogenic, bacteria, toxins, viruses, prions or parasites. Such contamination was caused by improper food handling, preparation or storage of food. Contacts between food and pests, especially flies, cockroaches and rodents are a further cause of contamination of food. Foodborne illness can also be caused by adding pesticides or medicine to food or consuming or by accidentally consuming naturally poisonous substances. That is why foodborne illness can also be called food poisoning.
Food-borne transmission refers to any illness that results due to the consumption of contaminated food, pathogenic bacteria, viruses, or parasites that contaminate food, as well as chemical or natural toxins such as poisonous mushrooms (cdc.gov). Bacteria is the most often the pathogen that causes food-borne illness. This is usually due to improper handling of foods, improper preparation of food and improper food storage. According to the CDC, the top 5 contributing pathogens to food-borne illness are Norovirus, non-typhoidal Salmonella, Clostridium perfringens, Campylobacter spp. and Staphylococcus aureus.
Food borne illnesses are caused by consuming contaminated foods or beverages. There are many different disease-causing microbes, or pathogens. In addition, poisonous chemicals, or other harmful substances can cause food borne illnesses if they are present in food. More than two hundred and fifty different food borne illnesses have been described; almost all of these illnesses are infections. They are caused by a variety of bacteria, viruses, and parasites that can be food borne. (Center 1)
Through various observations and assumptions, there have been conversations on whether or not overconsumption of food is an addiction. However, many have come to the conclusion that food is an addiction if it is overconsume excessively than it is necessary for an individual’s diet. Nevertheless, food addiction can create health issues that can affect an individual’s body. Even though, many individual may have such knowledge of overconsumption of food; however, many may choose to ignore the consequences that comes with food addiction. Becoming a food addict is harmful and dangerous to an individual’s health.
A SWOT analysis of the food control system in Bahrain revealed that certain strengths and weaknesses are inherent in the system. In addition to the weaknesses and the strengths, there are threats that would negatively affect the system if not prevented or brought under control. Nevertheless, the there are opportunities available for responding to the threats, making the Bahrain food control systems more efficient and effective. It is therefore important that some or all of these strengths, opportunities, threats, and weaknesses are reviewed. Conspicuous among the weaknesses is the fact that limited resources are available for the agencies and the personnel employed in the Bahrain food control system. Related to lack of resources is the lack of skills and competencies in applying modern techniques, more so in microbiological and chemical analysis. The second weakness of the Bahrain food control system is that most of the laws and regulations on food safety and control are not based on risk- or science-based analysis. In other words, the laws could be outdated and irrelevant in comparison with the latest mechanisms by which pathogens and other contaminants affect foodstuffs (Nestle, 2007). Furthermore, Bahrain lacks the technical expertise or competent enough personnel who could assess the effectiveness and the applicability of their food control laws.
Food waste is defined by food that is lost, wasted, or discarded and is caused through a few different processes. Food is wasted through many different ways at farms, grocery stores, and in homes. Over 40% of the food in the United States, specifically, goes to waste and 97% percent of this waste goes straight to landfills. The main issue with food waste is that the majority of food is being wasted unnecessarily. In stores, food is often thrown out because it does not meet specific standards that dictate what may be desirable to consumers. This issue of food picking is important as foods are not always being thrown away because they have gone bad, but because they have appearances that do not seem attractive. If we hope to lessen the environmental impact we have as a result of the mass