Electrophoresis Lab Report

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Testing of Intercellular Material for DNA through Agarose Electrophoresis
Purpose: The point of this lab was to determine whether or not DNA was actually extracted in the prior week’s experiment, in which E. Coli bacteria’s was lysed and through a series of chemical extractions it’s inner contents were harvested.
Methods: 4.5 mL E.Coli EDTA suspension was pipetted into a conical tube. After this 0,25 mL lysosome solution was put inside the same tube. Both were incubated at 37°C for so minutes. Once out of the incubator, 0.5 mL of 10% SDS was added. In order to ensure a good mixing of the liquids the tube was inverted continuously for several minutes. Incubation occurred once more but this time at 60°C for 10 minutes. Once cold down to room
It was loaded into the gel instead of in the well leading to no results. There should have been a series of bands similar to well three. In lanes three, 5, and 6 we see the bands labeled A,B and C. Due to the short distance traveled down can discern that these bands are in fact DNA as it is both bulky and negatively charged. In lane seven one can see the band in the beginning, which is identified, as tRNA. E and F represent the left of “junk” from the inside of the cell that made it’s way into the sample such as mRNA and proteins. This can be told by seeing that the small materials traveled very far down the gel and were not removed by DNase. What truly tells one whether or not he or she extracted DNA are the blank spots on the gel. In lane four and eight there are missing bands. This is due to the fact in these samples enzymes where added to break down the nucleic acids, DNase in the case of lane four and RNase in eight, thus causing a gap where they should appear. The data that was collected seems to indicate that the sample that was extracted was done properly and yielded DNA. It should be noted that the lanes three and four were switched when adding the material into

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