Dna Testing Lab Report

7. Next, my lab assistants and I leveled the gel rig using small levels. I placed the levels on the surface where I would put the casting tray. We did this to make sure the gel hardened evenly.
8. We put the taped tray into the gel rig. Then, I put the comb into the casting tray on the edge of the tray. By putting the comb into the tray, this made sure the gel would have compartments to put the DNA into when the gel hardened and the comb was taken away. Afterwards, we put the gel rig on the counter and left it.
9. When the agarose had finally cooled, I poured the agarose into the gel casting tray that we placed aside earlier. We double checked that the comb was still inside the tray.
10. To make sure my experiment was not messed with, I wrote my lab assistant’s names and my own on a piece of paper, and placed it near

I grabbed three microtubules and labeled the tops. I labelled them as
CS (crime scene DNA)
S #1 and S #2
We used microtubules because this is where the suspect’s DNA will be placed. Then, we labeled them to make sure that we wouldn’t mix up the suspect’s DNA. My lab assistants and I put our names on them as well so we did not lose them. Afterwards, we placed them in a Styrofoam microtube holder so that the tubes would sit upright. Next, we put a piece of masking tape on top and labeled this holder with our names.
2. To transfer the DNA to the microtubes, we used a micropipette. It is a device that sucks up the perfect amount of DNA for each tube. Also, the micropipette has disposable tips, so after each use, we can get rid of the tip so we don’t contaminate our experiment. We used a fresh tip for each sample. With a new tip each time, I grabbed of each DNA sample and placed it into the correct labelled test tube.
3. Then, using a fresh tip each sample, I transferred of the enzyme to each separate tube of the DNA samples. By adding the enzymes, this will cut the DNA molecules into small pieces when we place it into the gel and let it