Yeast Temperature Lab

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Observe the effects of temperature on the reaction of hydrogen peroxide and water with the presence of yeast.
Introduction:
In this lab the group studied the effect of temperature on rate of chemical reaction between hydrogen peroxide and water in the presence of yeast. This lab used yeast and the enzyme it possessed to act as a catalyst between the hydrogen peroxide and the water.
Enzymes are proteins that help facilitate the reaction of chemicals together by lowering the needed amount of energy (Campbell, pg. 83). The experiment tests the reaction rate of hydrogen peroxide, water, and yeast at different temperatures. Naturally as hydrogen peroxide and water combined the chemical reaction will release oxygen gas as a product. The oxygen gas …show more content…

The controls for the experiment are the amounts of hydrogen peroxide, water and yeast. The yeast contains an enzyme that will lower the needed amount of energy to create a chemical reaction between hydrogen peroxide and water.

Eckholm, Randine
K
BIOL 160
5Feb15
Enzymes Lab, Lab 5
Method:
The group labelled 4 test tube 1 through 4. Each test tube was filled with 5mL of 3.0% of hydrogen peroxide and 3mL of water. Test tube 1 was placed in an ice bath, test tube 2 was placed in the test tube holder, test tube 3 was placed in a warm water bath, and test tube 4 was placed in a hot water bath. All four test tubes were left set for 10 minutes. 10 drops of the yeast was added to test tube 1 and poured into a 250mL Nalgene. The Nalgene was swirled and the
O2 sensor was attached to form a seal around the top. When the test tube was poured into the
Nalgene the stopwatch was started and date was collected after 30 seconds and until 180 seconds. The data and rate of O2 production was collected. The group then repeated the same steps for all other test tubes.
Discussion:
In our results the groups hypothesis was confirmed. The results of O2 production in test tube 1, which was at 0° C produced very little )2. As the temperature increased in test tube

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