Macromolecules Lab Report

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Macromolecules
In the Macromolecules lab we tested for different thing such as reducing sugar, starch, and protein. In the reducing starch experiment we tested three different macromolecules. Each macromolecules was tested for sugar and how we indicated how much sugar was in each macromolecules was because the color would change. Some of the colors it would change to would be light blue (no sugar), light green (a trace of sugar), yellow (low sugar) Orange (moderate), red orange (high). Seven tubes were tested that had 10 drops of a different liquid such as onion juice, potato juice, sucrose, glucose, distilled water, reducing sugar, and starch solution. In the Iodine starch test water, starch, milk, tofu, and apples was tested. The blue indicated that there was starch and yellow indicating there is no starch. In the biuret test for protein …show more content…

Set up tubes; labels
2. Add 1 mL of each sample to be tested. Make sure you stir the solution before pipetting it into your tube.
3. Add 2 mL of Benedict`s reagent to each tube
4. Place the tubes in a baker of boiling water for 3 minutes.
5. Remove the tube and allow them to cool
6. Examine each tube and record the solution color in the table
7. Rinse out the tubes; use the same labels for the iodine test
Iodine Test for Starch
1. Set up tubes and label
2. Add 1 mL of each sample to be tested. Make sure you stir the solution before pipetting it into your tube
3. Add 7-9 drops of iodine (IKI) to each tube. DO NOT heat
4. Examine each tube and recorded the color in the table
Procedure for the Biuret Test for Proteins
1. Set up tubes; label
2. Add 2 mL of 2.5% sodium Hydroxide (NaOH0 to each tube
3. Add 2 mL of each sample to be tested
4. Add 3-5 drops of Biuret reagent to each tube, mix well and let settle for 2 minutes.
5. Examine each tube and recorded the

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