Yeast Enzyme Lab Report

Enzyme Lab Report

Kyle Zalatar

Sach Dubey

Brandon Chen

Damon Che

Dougherty Valley High School

Abstract

The purpose of this experiment was to witness how enzymes act in different conditions for instance how enzymes would react in different temperatures or a different ph level.We found that any temperature which is not the standard for the enzyme will lower the productivity. For the first lab we represented our hands as catalysts and we were connecting pop beads blindley to record how effective we were, in the second trial we put on gloves to simulate how enzymes react in different temperatures, we found out enzymes are less productive in different temperatures. The second lab was more literal we used yeast as our enzyme and we put paper

This experiment also needed 4 students, one being in charge of the yeast, another being in charge of the solutions and stirring rod, another one being the timer, and the last student writing down data. In order to make the filter paper float up to the top, there were twenty holes punched and the tweezers were used to pick up one piece at a time and soaked in the yeast for 5 seconds each time. After the paper was taken out of the yeast, it was transferred onto the stirring rod and pushed all the way to the bottom of the test tubes and used the timer to measure how long it took to float back up. This was done five times to all three different pH waters and calculated the average of each one to get better

The claim stated that the reaction rate of an enzyme will decrease when the pH is changed. The data(see Table 1) supports this hypothesis. The reaction rate of the enzyme decreased when the pH was affected. After 180 seconds of testing, the number of Pop Beads metabolized dropped from 31 in normal conditions to 8 when affected by a change in pH. Secondly, in the yeast lab, the data taken displays the effects of temperature, pH, and concentration on the reaction rate of catalase. The claim stated that changing these variables will result in a decreased rate of reaction. The data refutes this claim. Over the course of five trials, the reaction rate of catalase did not decrease after manipulating variables of temperature, pH, and concentration(see Table 2). This is evident because the time it took for the paper to rise did not decrease at all when variables were