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Fermentation by yeast
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Fermentation investigation yeast
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In our Yeast Fermentation Experiment, my group and I investigated the optimal temperature and sucrose concentration for yeast fermentation. To test for the optimal temperature for yeast fermentation, we measured the amount of CO2 that was produced inside fermentation tubes that were heated to varying temperatures. By measuring which temperature produced the most CO2, we were able to determine that 60 degrees Celsius was the optimal temperature for yeast fermentation to occur. We tested for optimal sucrose concentration the same way we did for optimal temperature except we used varying amount of sucrose instead of varying temperatures. After this test we were able to conclude that 2 grams of sugar was the optimal sucrose concentration for yeast …show more content…
The variable in this experiment was sucrose concentration instead of temperature, so instead of having beakers at different temperatures, we had one bath set at 60oC, which was the temperature we found to be the optimal temperature of yeast fermentation. Our main difference for this experiment was instead of adding 2.5 grams of sugar in each sugar/yeast solution, we added 1.5, 2, 2.5, 3, 3.5, and 4 grams. The various solutions were placed in fermentation tubes just like the experiment before. They were then sealed and placed in the 60oC bath. The amount of CO2 was measured every 5 minutes for 20 minutes. This experiment was repeated twice to make sure our data was consistent. From this experiment, our group was able to determine that 2.0 grams of sugar in the sugar/yeast solution was the optimum …show more content…
This suggests that either an error could have occurred during the process of the experiment, or that this certain experiment does not yield consistent results. It is possible that this could be explained if this experiment was tested a few more times. It is likely the factors that could have caused inaccurate results in the first experiment could have caused inaccurate results in the second experiment as well. Miscalculations, dirty equipment, and other factors of that nature could have played a role in the inconsistent results our group experienced in the first and second test of the optimum sucrose concentration
If this experiment were designed to determine the amount of Fructose in a solution, describe what, if anything, would need to change in the reaction? Explain why there would or would not need to be changes. (5
Extraction is a separation method that is often used in the laboratory to separate one or more components from a mixture. Sucrose was separated at the beginning because it is the most immiscible and it’s strongly insoluble. Next Acetylsalicylic Acid was separated which left Acetanilide alone. Variety steps could have led to errors occurring. For example the step of separation, when dichloromethane layer was supposed to be drained out, it could be possible some aqueous layer was drained with it. Which could make the end result not as accurate. Also errors could have occurred if possibly some dichloromethane was not drained out. Both way could interfere with end result of figuring the amount of each component in the mixture. The solids percentage were 22.1% more than the original. That suggests that solids weren’t separated completely which clarifies the reason the melting points that were recorded were a slightly lower than the actual component’s melting point. The melting point for Acetylsalicylic Acid is 136 C but that range that was recorded during the experiment was around 105 C to 118 C. The melting points were slightly lower than the literature value. Sucrose was the purest among all component due to its higher melting point which follows the chemical rule that the higher the melting point the more pure the component
Table 6 shows the results of the biochemical tests. The isolate can obtain its energy by means of aerobic respiration but not fermentation. In the Oxidation-Fermentation test, a yellow color change was produced only under both aerobic conditions, indicating that the EI can oxidize glucose to produce acidic products. In addition to glucose, the EI can also utilize lactose and sucrose, and this deduction is based on the fact that the color of the test medium broth changed to yellow in all three Phenol Red Broth tests. These results are further supported by the results of the Triple Sugar Iron Agar test. Although the EI does perform fermentation of these three carbohydrates, it appears that this bacterium cannot perform mixed acid fermentation nor 2,3-butanediol fermentation due to the lack of color change in Methyl Red and Vogues-Proskauer
2. A test tube was then filled with 35ml of yeast and placed in the
Rate of Respiration in Yeast Aim: I am going to investigate the rate of respiration of yeast cells in the presence of two different sugar solutions: glucose, sucrose. I will examine the two solutions seeing which one makes the yeast respire faster. I will be able to tell which sugar solution is faster at making the yeast respire by counting the number of bubbles passed through 20cm of water after the yeast and glucose solutions have been mixed. Prediction: I predict that the glucose solution will provide the yeast with a better medium by which it will produce a faster rate of respiration. This is because glucose is the simplest type of carbohydrate (monosaccharide).
In this experiment, there were several objectives. First, this lab was designed to determine the difference, if any, between the densities of Coke and Diet Coke. It was designed to evaluate the accuracy and precision of several lab equipment measurements. This lab was also designed to be an introduction to the LabQuest Data and the Logger Pro data analysis database. Random, systematic, and gross errors are errors made during experiments that can have significant effects to the results. Random errors do not really have a specific cause, but still causes a few of the measurements to either be a little high or a little low. Systematic errors occur when there are limitations or mistakes on lab equipment or lab procedures. These kinds of errors cause measurements to be either be always high or always low. The last kind of error is gross errors. Gross errors occur when machines or equipment fail completely. However, gross errors usually occur due to a personal mistake. For this experiment, the number of significant figures is very important and depends on the equipment being used. When using the volumetric pipette and burette, the measurements are rounded to the hundredth place while in a graduated cylinder, it is rounded to the tenth place.
This shows that there could be three variables in this experiment, carbon dioxide, water and light energy. So in our case the variable light energy (light intensity) will be used. The equation also shows that if there is more light energy then more glucose and oxygen will be produced.
Consequently the weight of the sucrose solution bags increased the greatest. The results of this experiment were what was expected and proved the hypothesis to be true because the sucrose solution was of lower concentration due to the fact it increased in weight the most. Thus, the more water moved through the semi permeable membrane compared to the others. If there was anything to change with this experiment, it would be procedurally. I would be sure to use different semi permeable membrane bags that don’t allow water to leak due to the fact that both ends were only closed with a rubber band and it was difficult to ensure the bags were tightly sealed. This could help prevent
The Effects of Concentration of Sugar on the Respiration Rate of Yeast Investigating the effect of concentration of sugar on the respiration rate of yeast We did an investigation to find how different concentrations of sugar effect the respiration rate of yeast and which type of concentration works best. Respiration is not breathing in and out; it is the breakdown of glucose to make energy using oxygen. Every living cell in every living organism uses respiration to make energy all the time. Plants respire (as well as photosynthesise) to release energy for growth, active uptake, etc…. They can also respire anaerobically (without oxygen) to produce ethanol and carbon dioxide as by-products.
== == == = This is what I'm going to be changing in the experiment and this will be the temperature and the concentration of the yeast. There are several variables in this experiment, they are: · Amount Used - Too much or too little of the hydrogen peroxide causes the reaction to speed up/slow down producing different amounts of oxygen.
Conclusions: There is a pattern on the graph, and data table, which shows that as the concentration of the sucrose solution increases, the potato's percentage change in mass decreases.
This lab attempted to find the rate at which Carbon dioxide is produced when five different test solutions: glycine, sucrose, galactose, water, and glucose were separately mixed with a yeast solution to produce fermentation, a process cells undergo. Fermentation is a major way by which a living cell can obtain energy. By measuring the carbon dioxide released by the test solutions, it could be determined which food source allows a living cell to obtain energy. The focus of the research was to determine which test solution would release the Carbon Dioxide by-product the quickest, by the addition of the yeast solution. The best results came from galactose, which produced .170 ml/minute of carbon dioxide. Followed by glucose, this produced .014 ml/minute; finally, sucrose which produced .012ml/minute of Carbon Dioxide. The test solutions water and glycine did not release Carbon Dioxide because they were not a food source for yeast. The results suggest that sugars are very good energy sources for a cell where amino acid, Glycine, is not.
Investigating the Effect of Temperature on the Fermentation of Yeast To fully investigate the effect of temperature on the rate of fermentation of yeast Background Information Yeast is a single-cell fungus, occurring in the soil and on plants, commonly used in the baking and alcohol industries. Every living thing requires energy to survive and through respiration, glucose is converted into energy. There are two types of respiration available to living cells are: 1.
One major change I would make into the procedure is the to make a more efficient way to extract the sugar. To make it more efficient fi...
There is also the potential of human error within this experiment for example finding the meniscus is important to get an accurate amount using the graduated pipettes and burettes. There is a possibility that at one point in the experiment a chemical was measured inaccurately affecting the results. To resolve this, the experiment should have been repeated three times.