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Essay on the lab report
Essay on the lab report
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Glory Nguyen
October 20, 2015
Period G
Dissolving Oxygen
Introduction In this lab, you will be testing the levels of oxygen in water samples with different temperatures and water samples exposed to different light distances. This will show us where the most oxygen is produced, which is where most of the organisms are. The relationship between oxygen and carbon assimilation is when oxygen is being produced the the photosynthesis plants, they release oxygen. In turn, it is then being inhaled by an organism that needs oxygen, releasing carbon as carbon fixation. So more carbon means more oxygen being released. The concentration of dissolved oxygen is obviously a lot higher during the afternoon, this is when the sun is out and where photosynthesis
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Record the temperature of the cold water sample onto Table #1
Immerse the test tube under the cold water and stop in with a rubber stopper (while under water).
Remove the rubber stopper and add 6 drops on Winkler's solution #1 up into the test tube with cold water.
Then add 6 drops of Winkler's solution #2 into the test tube with cold water.
Place the rubber stopper back on the test tube with cold water; careful for spillage.
Invert the test tube with cold water several times to mix the solution (brown precipitation will form).
Allow the precipitate to settle for 5-10 minutes.
Repeat steps 2-8 with the room temperature and warm temperature samples.
Remove the stoppers and add 6 drops of concentrated sulfuric acid to each of the test tubes.
Put the rubber stoppers back on the test tubes and invert them; this dissolves the brown precipitate to a yellowish-amber solution.
Then titrate each of the fixed water samples. *In the case that you don't know how to titrate, refer to the bottom of the page.
Method/ Procedure (for Part
Start with the hot water and first measure the temperature. Record it. 8. Then pour 40 ml into the beaker. You can measure how much water was used by looking at the meniscus.
We then took 1ml of the 0.1% solution from test tube 2 using the glucose pipette and added it to test tube 3, we then used the H2O pipette and added 9ml of H2O into test tube 3 creating 10ml of 0.01% solution.
5.) One at a time, place your test tubes in the water bath and heat the first test tube to 25 , the second to 50 , the third to 75, and the last to 100 degrees c. Remeber to stir with your stirring rod every so often.
The procedure of the lab on day one was to get a ring stand and clamp, then put the substance in the test tube. Then put the test tube in the clamp and then get a Bunsen burner. After that put the Bunsen burner underneath the test tube to heat it. The procedure of the lab for day two was almost exactly the same, except the substances that were used were different. The
For this experiment, you will add the measured amount of the first sample to the measured amount of the second sample into its respectively labeled test tube then observe if a reaction occurs. In your Data Table, record the samples added to each test tube, describe the reaction observed, if any, and whether or not a chemical reaction took place.
Repeat for each trial. Rinse volumetric pipette with vinegar and drain into the waste beaker. Weigh and record the mass of each 200mL beaker. Add 10.00mL of vinegar into each beaker and weigh them and record their again. Add 50mL of de-ionized water to the beakers and place them under the drop counter on top of a stir plate, submerging the pH meter into the solution. Place the stir bar into the beaker and carefully turn on the stir plate so that the stir bar spins without splashing or hitting the sides of the beaker or the pH
10. Point the flask away from everyone and open the two-way valve in order to release pressure from the flask. Remove the stopper assembly, then fill up the flask with water. Discard of the solution in the sink.
6. Place the test tube in the beaker. Secure the test tube and thermometer to the retort stand using clamps. Begin heating the water bath gently.
Attach a buret clamp (located under the hood) to a ring stand. b. Rinse the burets three times with approximately 10 ml of deionized water. Tilt and rotate the buret in an almost horizontal position (don't let the water spillout!) to rinse the entire inside wall. Allow about 5 ml of water to run through the buret tip on the last rinse. c. Pre-rinse one buret with approximately 5 ml of your Unknown acid solution. Again, rotate the buret to rinse the entire inside wall of the buret as above. d. Clamp the buret in one side of the buret clamp. Place a white piece of paper labeled "Unknown acid" under this buret. Drain any remaining pre-rinse acid solution into a beaker labeled "waste solution". e. Fill this buret with your Unknown acid solution to the zero mark or slightly below it (Not above the zero mark). Make sure the tip of the buret is completely filled and contains no air bubbles. f. Pre-rinse the second buret with approximately 5 ml of standard base solution. Clamp the buret in the other side of the buret clamp. Place a white piece of paper labeled "Standard NaOH solution" under the buret. Drain remaining prerinse NaOH solution into the waste solution beaker. Fill this buret with standard
After five the test tube was removed and cooled to room temperature. Three more test tubes were obtained and labeled 1, 2, and 3. The correct reagent was added to each test tube as seen. The spectrophotometer was adjusted
Begin collecting samples with the pure hexane. Keep adding hexane so that the silica gel column does not run dry. Collect one 20 ml sample. Repeat with 90:10 hexane and collect 4 20-mL bottles. Repeat with 80:20 hexane and collect 2 20-mL samples.
2. In the large beaker, put water and boil it completely. After that, remove the beaker from heat. 3. Sample tubes (A-D) should be labeled and capped tightly.
In a 100ml beaker place 50mls of water, measure the temperature of the water and record this initial temperature onto a table. Set the timer and add one teaspoon of Ammonium Nitrate to the water, stir this continuously until the Ammonium Nitrate has dissolved.
== § Test tubes X 11 § 0.10 molar dm -3 Copper (II) Sulphate solution § distilled water § egg albumen from 3 eggs. § Syringe X 12 § colorimeter § tripod § 100ml beaker § Bunsen burner § test tube holder § safety glasses § gloves § test tube pen § test tube method = == = =
tube. Add 6 mL of 0.1M HCl to the first test tube, then 0.1M KMnO4 and