Sterilization And Disinfectionation In Stevia

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Experimental Plant Material:
The healthy plant of Stevia rebaudiana for micropropagation has been selected from the garden of Integrated Biotechnological Research Institute, Lucknow. The aseptic conditions were maintained following suitable sterilization methods.

Sterilization and Disinfection:
Since we have known so far about the ways of sterilization,here is a review about sterilization and techniques involved for plant sterilization.Sterilization is the process where all the living microbes, including bacterial spores are killed. Sterilization can be achieved by physical, chemical & physiochemical means. Chemicals used as sterilizing agents are known as chemisterilants. Disinfection is the process of removal of most pathogenic microorganisms (excluding bacterial spores) on inanimate objects. Disinfection can be achieved through physical or chemical methods. Chemicals used in disinfection are known as disinfectants. Various disinfectants have different target ranges, not all disinfectants can kill all microorganisms. Some process of disinfection such as filtration do not kill bacteria, they separate them out. Sterilization is an absolute condition while disinfection is not. The two are not same terms. Decontamination is the method of removal of contaminating pathogenic microorganisms from the articles by a process of sterilization or disinfection. It is the use of chemical or physical means to, inactivate,remove or destroy living organisms on a surface so that the organisms are no longer infectious. Sanitization is the process of mechanical or chemical cleansing, applicable in public health systems. Usually used by the food industry & it reduces microbes on eating utensils to safe, acceptable levels for public health. Aseps...

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...okinin.
(2)Incubate the cultures in dark at 25oC. Callus will be produced in 3-4 weeks.
(3) Compare the callus induction growth from various explants.
(4) Cut small pieces of callus (0.5 gm) fresh weight and subculture on the same fresh medium for proliferation.

Culture medium and culture condition for shoot multiplication
MS media composition, sucrose (30 g/l) and bacteriological grade agar-agar (8 g/l) were used throughout the study. For shoot induction, growth regulators, BA alone or in combination with IAA, were added to the MS basal medium. For root induction, strength MS basal medium supplemented with either IAA or IBA and the pH was adjusted 5.8. All the cultures were incubated in a culture room maintained at 25°C under 16 h/8 h light/dark cycle, 45 µmol m-2 s-irradiance level provided by cool white fluorescent tubes and with 55 - 60% relative humidity.[6]

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