Personal Safety When Using Bacteria And Agar Platess

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Safety when using microbiology is very important, hazards can be anything from bags in the way, drinks or food on the work bench, wounding, contamination, ingestion. All these are dangerous when using Bunsen burners and bacteria. A few ways to avoid these hazards would be to first tidy the work bench disinfect the area where you will be working, move any clutter, bags, phones, food and drink. You don’t want any personal items coming in contact with the bacteria. Disinfecting the area is important so you don’t get any cross contamination when using bacteria and agar plates. Personal safety to ensure hair is tied back you wouldn’t want your hair to catch fire or be dipped in any solutions. Always make sure lab coats and goggles are on to ensure …show more content…

Spread plates are mainly used with a diluted solution or mixed with microbes so that individual colonies can be isolated. Spreading 0.5ml of a sample over the agar jelly with a L shaped glass spreader to ensure that the bacteria is spread as even as possible over the full surface of the agar plate to separate the bacteria. This method is used to count the number of colonies on the surface one advantage is that cultures are never exposed to more than 45oC .

Contamination
Cross contamination in the health care setting can happen in a number of different ways, from eating and drinking, smoking, applying cosmetics, preparing food, performing clean r sterile procedures, working with patients.
A patient’s environment in the health care setting can also be a place of cross contamination, the most problematic area for cross contamination is a patient’s entire bedside area. This area includes the bed, bedside table, bed frame and rails, bed clothes pillows, sheets, clothing and bed side chairs.
Three main control measures that are applied in the healthcare setting are to clean and disinfect everything that is touched, cooked with, handled, patients come in to contact with. Regular hand washing is essential for any health care setting along with the exclusion and chortling of ill …show more content…

The sources of infection from these bacteria are usually contaminated food and water. People can be infected by ingestion of contaminated food or water especially undercooked chicken or contaminated water. Contact with infected animals especially puppies or kittens with diarrhoea, poor food handling especially the same chopping boards, knives, plates for raw and cooked food.
Campylobacter food poisoning symptoms usually last 2 to 5 days. These include diarrhoea, severe abdominal pain, vomiting and fever. Campylobacter is a serious disease in indigenous communities because of the possibility of dehydration from diarrhoea.
The bacteria in campylobacter reproduce by splitting in half, when they are split in half they have multiplied in the right conditions they can multiply at a very fast rate. The ideal temperature for bacteria to multiply is 37oc this is human body temperature. Moisture around the bacteria helps the bacteria to grow along with other sources of food supply around that can be contaminated or already may be contaminated. Bacteria in the right conditions can multiply every twenty minutes, if a piece of meat is left out in a hospital kitchen and has 100 food bacteria on it and was left lying on a kitchen bench on a warm day the bacteria would double their number every 20 minutes. After returning to the meat three hours later the 100 food poisoning bacteria will have multiplied to

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