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Investigating osmosis in living plant cells
Investigating osmosis in living plant cells
Investigating osmosis in living plant cells
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Investigation to Find the Water Potential of a Root Vegetable
Introduction
Water potential is the tendency of water molecules to move from one
place to another. Osmosis is the movement of water molecules from a
region of higher water potential to a region of lower water potential
through a partially permeable membrane. I will be investigating the
water potential of a carrot to find out at what concentration of salt
solution (molar dm-3) equilibrium can be sustained between the net
movement of water molecules in to the carrot cells, and the net
movement of water molecules out of the cells, therefore finding out
the water potential of the carrot, and at what concentration of salt
solution the movement of water molecules ceases, and what
concentration the water is at inside the carrots cells. I will need to
include and explain the relevant AS knowledge demonstrated by this
investigation. This includes ideas about osmosis, explaining also how
this can affect the structure of plant cells, and showing extremes
such as plasmolysis.
Sill P - Planning
Prediction
I predict that as the concentration of salt solution (molar dm-3) is
increased, meaning that the water potential outside the carrot
decreases, the water potential inside the carrot will decrease. As the
water potential outside of the carrot cell is decreased, the water
potential inside the carrot will increase, when the salt solution is
more dilute. This change in water potential will occur because of a
net movement of water molecules called osmosis.
Osmosis is; The movement of water molecules from an area of high water
potential to an area of low water potential, down the concentration
gradient, across a partially permeable membrane.
A partially permeable membrane, which is found in plant cells, such as
the ones in a carrot, is a membrane that only allows certain molecules
to pass through it, in this case, the membrane will allow water
molecules to pass through it, but will not let the salt molecules pass
through. Therefore, I predict that; when the water potential is higher
Investigating the Water Potential of Celery Cells Aim = == I will be investigating the water potential of celery and to find out which solution will be isotonic with the celery cells, in other words equilibrium between the two no water will leave the cell, or enter. I will do this by following this method. Method 1.
Osmosis Experiment Planning Aim: The main subject that I will be planning to investigate is the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory. Background knowledge: The plant cell and its structure To understand osmosis in detail I will need to explain the plant cell (which is the cell included in the osmosis experiment) and its cell membrane. Below I have a diagram of a plant cell: [IMAGE] Osmosis is about the movement of particles from a higher concentrated solution to a lower concentrated solution to create an ethical balance via a partially or semi permeable cell membrane. Osmosis in simple terms is the exchange of particles between the cytoplasm inside the cell and the solution outside the cell. What makes this exhange is the partially permable cell membrane.
The average length of a cell in tap water is 90.0588 micrometers and the average length of a cell in a 10% salt water solution is 75.1838. In comparison the differences between the averages are striking. Demonstrating that the length of the cell shrinks and is undergoing plasmolysis when the salt water solution was introduced to the previously standing cells in tap water. Plasmolysis is the shrinking of the cytoplasm away from the cell wall. Plasmolysis is occurring because when the 10% salt solution is introduced to the elodea leaf the cells in the elodea leaf are submerged in a hypertonic solution. Meaning that there are more solutes outside the cell rather than inside the cell. The solution is hypertonic because the NaCl which composes salt has a high electronic pull. Causing the H2O in the water to attract to the NaCl. When submerged in the salt water solution the elodea leaf cells water is being attracted to the NaCl electric pull so by diffusion the water is pulled out of the
Osmosis in Potato Tubes Osmosis: Osmosis is the movement of water molecules through a semi-permeable membrane from a high concentration to a low concentration. Diagram: [IMAGE] [IMAGE] Aim: To see the effects of different concentration of sugar solution on Osmosis in potato tubes. Key factor: In the investigation we change the sugar solution from: 0%-10%-20%-30%-40%-50% this is the independent variable; the dependant variable is the change in mass. Prediction: I predict that all the potato tubes in pure water or low concentration sugar solution will swell because water enters their cells by osmosis.
Osmosis in Carrots Background Osmosis is the diffusion of water from a dilute solution to a more concentrated solution through a partially permeable membrane, which allows the pass of water molecules but not solute molecules. [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE]If a cell is placed in a less concentrated solution water enters because the less concentrated solution will have a high concentration of water than the inside of the cell. Once the cell takes in maximum water the cell becomes turgid. If the cell was to be placed in a high concentrated solution, water would leave the cell because the cell would contain a low concentrated solution. So in the low concentrated solution there will be a high concentration of water and in the high concentrated solution there will be a low concentration of water.
When I am not using them I will place them away from my experiment and
potato. To make it a fair test I will make sure that the tests will be
If a plant cell is places in a hypotonic solution the cell has a lower water concentration to that of the solution. Water will move into the cell by osmosis from a high water concentration outside the cell to a lower water concentration inside the cell through a selectively permeable membrane. The cell becomes turbid
Water Potential of Potato Cells Aim: To demonstrate the Water Potential of Potato Cells. Objectives: · To show the water potential of potato cells using various measured concentrations of a sucrose solution and pieces of potato. · To record and analyse data to verify observed results. · The method and procedure was carried out as per instruction sheet. Observations: The experiment shows that the lower the concentration of the sugar solution, in the Petri dish, the mass of the potato increased.
My plan is too find the percentage change of mass of a small piece of
Potato cells, diffusion, osmosis, and tonicity by Michaela Cupp. Science Experiment Topic: Which solution will help absorb the red dye into the potato cells, pure water or 50% salt water? Related research topics/terms: Diffusion, Osmosis, Tonicity are things I found during my experiment. An explanation of the links between the topics:
Investigation of Factors Affect Osmosis in Potatoes Aim The aim of the following experiment was to investigate the effect of varying the concentration of sucrose solution on osmosis in a potato. Preliminary Experiments One preliminary experiment was done before the main experiment. From the preliminary, we were trying to find out how osmosis actually occurred in potatoes, and gave us a vague idea on what the main experiment would be like. This preliminary will aid my prediction, which is stated below. The following apparatus was used for the preliminary: * 1 large potato (skin intact) * 3 boiling tubes * Set of cork borers * Scalpel * Balance (accurate to 2 decimal places) * Distilled water * 0.5M sucrose solution * 1.0M sucrose solution * Dropping pipette * Boiling tube rack * Measuring cylinder (accurate to 1cm3) * White tile Take a large uncooked potato, with the skin still on, and with the cork borer, cut out three "tubes" of potato.
Osmosis is the passage of water molecules from a weaker solution to a stronger solution through a partially permeable membrane. A partially permeable membrane only allows small molecules to pass through, so the larger molecules remain in the solution they originated in. Solute molecule [IMAGE] [IMAGE] Water molecule [IMAGE] The water molecules move into the more concentrated solution. When water enters a plant cell it swells up. The water pushes against the cell wall and the cell eventually contains all that it can hold.
The Effect of Solute Concentration on the Rate of Osmosis Aim: To test and observe how the concentration gradient between a potato and water & sugar solution will affect the rate of osmosis. Introduction: Osmosis is defined as, diffusion, or net movement, of free water molecules from high to low concentration through a semi-permeable membrane. When a substance, such as sugar (which we will be using in the experiment we are about to analyse), dissolves in water, it attracts free water molecules to itself, and in doing so, stops them from moving freely. The effect of this, is that the concentration of (free) water molecules in that environment goes down. There are less free water molecules, and therefore less water molecules to pass across a semi-permeable membrane, through which sugar molecules and other molecules attached to them are too big to diffuse across with ease.
there would be no flow of water into or out of the cell so the cell