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Importance of Mendels law in modern genetics
Chapter 14 mendelian genetics
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Genetics defined as the study of heredity of genetic material from parents to offspring. In 1865, Gregor Mendel (the father of genetics) conducted experiments on pea plants led to the birth of genetics. He recorded his observation accurately by studying one trait at a time. Mendel used different variable factor during his experiments. For example, he used the height of the plant, the shape of the seed, and the color of the seed. He discovered three laws which called the Mendel principles. These laws were: law of dominance, law of segregation, and law of independent assortment (Michael J. Simmons, 2015). The law of dominance defined as one allele during heterozygote condition is dominate over the other allele. Meanwhile, the law of segregation …show more content…
Fruit fly has four life stages which are egg, pupa, larva, and adult. The generation time of fruit fly is two weeks at 21◦C. Egg and larva stages take eight days while pupa stage takes six days. That makes the adult fly to produce after two weeks. The wild type fly has dark red eyes, oval shaped eye, grayish body color, and straight wing shape. Fly with different phenotype than the wild type called mutant. Male and female flies have different characteristics in which the male flies have dark posterior part while females have lighter posterior part. Also, the male flies have a more rounded tip of abdomen than female flies (Carolina Drosophila Manual, …show more content…
Three pairs of autosomal chromosomes and one pair of sex chromosomes. Some mutant genes can occur on the sex chromosome. For example, Bar eye mutation, yellow body mutation, and white eye mutation all these mutations located on sex chromosome, specifically on the X chromosome. Also, some mutant genes can occur on the autosomal chromosomes. For example, ebony body color and sepia eyes mutation are located on autosomal chromosome number 3 while vestigial wing mutation is located on autosomal chromosome number 2. Chromosome mapping was used to demonstrate the locations of these genes. All autosomal mutations in fruit fly are recessive mutation which mean two alleles of the mutation should be expressing to exhibit the mutation in males and females. Yellow body mutation and white eye mutation are recessive sex-linked genes which means two copies of yellow or white mutations in female flies should be on X chromosome to exhibit the trait while one copy of these mutations in male flies should be on the X chromosome to show the trait. On the other hand, Bar mutation is a dominant mutation that located on the X chromosome, so that one copy of Bar mutation exhibits the trait in the male or female because it is a dominant mutation. As a result, male files can be either (B+/--) or (B/--) while the female flies can be either (B+/ B+), (B+/ B), or (B/B) (Kang, Mehdi & Minard, 2017, p. 3). Bar eye mutation in fruit fly demonstrates
In order to figure out the genes responsible, there are several other factors that must be determined. These factors include the number of genes involved, if each gene is x-linked or autosomal, if the mutant or wild-type allele for each is dominant, and if genes are linked or on different chromosomes. Proposed crosses include reciprocal crosses between the pure-breeding mutants of strains A and B with the wild-type will help determine if the genes or sex-linked or autosomal, in addition to which alleles are dominant (8). Another proposed cross includes complementation crosses between pure-breading mutants from strains A and B to determine if one or two genes are involved (8). Furthermore, testcrosses between F1 progeny and pure-breeding recessive mutants from strains A and B, which will help determine if genes are linked on the chromosome or if they assort independently (8). These proposed crosses are shown in the attached
17. Fruit flies normally have eight chromosomes. The diagram below shows the result of meiosis in three fruit flies to produce gametes with the number of chromosomes indicated. The male then mates with both female A and female B to produce three zygotes (1, 2, and 3).
The idea of the project was to experiment breeding Drosophila Melanogaster (fruit fly) to figure out if certain genes of that species were sex linked or not (autosomal). A mono-hybrid cross and di-hybrid cross was performed. For the mono-hybrid cross, white eyed female and red eyed male were placed in one vial for them to reproduce. For the di-hybrid cross, red eyed and normal winged flies and sepia eyed and vestigial winged flies were placed in their vial to reproduce. In the mono-hybrid cross the results expected were within a 1:1:1:1 ratio. Expected results similar to the expected desired null hypothesis proposed with what the F1 parental generation breeds. The potential results would have had to have been within the ratios of 9:3:3:1. The results were clear and allowed the null hypothesis to be correct. The white eyed gene in the fruit flies is sex linked. Sepia eyes and vestigial wings are not sex linked and are examples of independent assortment.
Studying fruit fly mating behavior is very important because their generation length is so short and reproduction happens rapidly. In science, these fruit flies can be used to study genes and mutations relatively quickly because of the limited life span. Knowing mating behaviors can help scientists better understand their results and improve their experiment designs to reduce
They are very popular because they have a relatively short life cycle, which lasts about 9 days. This makes it easy to observe multiple generations in a short period of time. The fly’s life cycle consists of egg, larvae, pupa, and adult stages. At each of these stages of development, Drosophila exhibits different phonotypical identities and mutations. The flies that were crossed contained a genetic mutation called apterous. Apterous can be observed by looking for flied that are missing wings. Flies with apterous will not have any wings. If this mutation is sex linked, it is expected that the male population will only be wingless in the F2 generation. If the mutational is autosomal, it is expected that the mutation to normal fly ratio is
The major topic of this experiment was to examine two different crosses between Drosophila fruit flies and to determine how many flies of each phenotype were produced. Phenotype refers to an individual’s appearance, where as genotype refers to an individual’s genes. The basic law of genetics that was examined in this lab was formulated by a man often times called the “father of genetics,” Gregor Mendel. He determined that individuals have two alternate forms of a gene, referred to as two alleles. An individual can me homozygous dominant (two dominant alleles, AA), homozygous recessive, (two recessive alleles, aa), or heterozygous (one dominant and one recessive allele, Aa). There were tow particular crosses that took place in this experiment. The first cross-performed was Ebony Bodies versus Vestigle Wings, where Long wings are dominant over short wings and normal bodies are dominant over black bodies. The other cross that was performed was White versus Wild where red eyes in fruit flies are dominant over white eyes.
Other groups had the same results that we ended up with which meant we were doing something right. This experiment was focused on fruit flies, however the same rules apply to humans and most living things, so we can learn where our traits were passed down to us and how. One of Mendel's laws is the law of inheritance and it is the law that is focused on in this lab and is important to our everyday life as
Gregor Mendel, an Austrian monk, introduced a new theory of inheritance based on is experiment with peas. Mendel’s genetic laws were called the Law of Segregation, the Law of Independent Assortment, and the Law of Dominance. Mendel worked with peas that were yellow or green in color and smooth or wrinkled in shape. The characteristics in the traits were distinct and similar; therefore, they gave Mendel the conception that his experiments would be easily tested. He concluded that the yellow pea plants, which bred with the green pea plants, had yielded all yellow peas; as a result, all the hybrid children looked like only one of the parents. Mendel theorized that genes can be made up of three possible pairings of heredity units called ‘factors’: AA, Aa, and aa. In Mendel’s crosses, the starting plants were homozygous AA or aa, the F1 generation was Aa and the F2 generation was AA, Aa, or aa. While Mendel’s research was with plants, the principles also applied to people and other animals. After Mendel’s death, his work was recognized, and he was regarded by the name ‘father of genetics’.
The fruit fly better known by geneticist as Drosophila melanogaster, is a very common model used to study genetic inheritance. It is perfect for studying inheritance patterns because of its tiny size and rapid reproduction. They also have many distinct characteristics between genders which makes viewing inheritance patterns easier. Males are small with dark bottoms and tend to have sex combs on their legs. Females are large with stripped bottoms, and pointed bodies. When dealing with the fruit flies they need to be kept in certain temperatures and the lab incubator/refrigerator helps sustain a good temperature. Like humans, the fruit fly has four pairs of chromosomes, one of which is the sex chromosomes.
5.Use a test-cross. Mate the unknown fly to a known homozygous recessive. Think about the results if the fly was heterozygous vs. if it were homozygous dominant.
The results of the fruit fly (Drosphila melanogaster) experiment undertaken are important for numerous reasons. Firstly, the results obtained give statistical insight into what the data values are showcasing in this experiment. The outcomes being depicted from a mathematical point of view makes it easier to comprehend. This laboratory activity demonstrates how count data gives a much better understanding of statistics (especially for genetics and evolution). It is better to obtain results from appropriate statistics rather than making conclusions based on data such as random sexual relations, genetic and evolutionary change and diversity of the fruit fly species. Secondly, the results summarize the data into an interpretation that is to the
In daylight environment sexual arousal decreased in w1118 compared to Ore R males. In the dark environment w1118 have a “scanning strategy” that allowed them to behave like wild type Drosophila.
Genetic testing has become very popular as technology has improved, and has opened many doors in the scientific community. Genetic testing first started in 1866 by a scientist known as, Gregor Mendel, when he published his work on pea plants. The rest was history after his eyes opening experiments on pea plants. However, like any other scientific discovery, it bought conflicts which caused major controversies and a large population disagreed with the concept of playing with the genetic codes of human beings. Playing God was the main argument that people argument that people had against genetics. genetic testing became one of the major conflicts conflicts to talk about, due to the fact that parents could now have the option of deciding if they
The effect of light and dark environments played a role in the phenotypic frequencies of the Drosophila melangaster. In the light environment, the red eye phenotypic frequency in both males and females steadily increased, while the white eye phenotypic frequency for both males and females steadily decreased over time with each measurement. Figure 1 shows a steady increase and decrease in the phenotypic frequency of the red and white eye allelic frequencies of males, which is evident by the sideways "v" shape of the graph. The red eye allelic frequency was high in both males and females in the light environment. In the dark environment, figure 1 shows that the male allelic frequency does change for both white and red eyes. The female phenotypic
A fly with just the “promoter-Gal4” is known as the driver line. The “UAS-GFP” alone in fly allows us to determine what specific foreign gene is introduced into the fruit fly’s genetic makeup. In this case, the “UAS-GFP” alone in flies allows us to determine that GFP is the incorporated foreign gene into the fruit flies genome, but it is not actively expressed. A fly with just the “ UAS-GFP” in known as the responder line. This allows for the crossing over of both lines of flies to show an expression of a specific gene at a specific tissue in their offspring and this allows to determine the effects of both components in the fruit