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Genetic traits of a common fruit fly
Fruit flies and studying modes of inheritance
Fruit fly experiments
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Recommended: Genetic traits of a common fruit fly
Introduction The fruit fly better known by geneticist as Drosophila melanogaster, is a very common model used to study genetic inheritance. It is perfect for studying inheritance patterns because of its tiny size and rapid reproduction. They also have many distinct characteristics between genders which makes viewing inheritance patterns easier. Males are small with dark bottoms and tend to have sex combs on their legs. Females are large with stripped bottoms, and pointed bodies. When dealing with the fruit flies they need to be kept in certain temperatures and the lab incubator/refrigerator helps sustain a good temperature. Like humans, the fruit fly has four pairs of chromosomes, one of which is the sex chromosomes. Experiments with the fruit flies were done to determine if certain patterns were dominant or recessive, and exemplified X-linked or independent assortment. When determining inheritance patterns it is necessary to use virgin female fruit flies, so that any tainted female flies were not infected with the wrong male flies. The female fly traits were called wild type, this particular pattern resulted in red …show more content…
This means putting all the flies to sleep by fly nap and taking them out to observe traits. Observe the traits under a microscope because the flies are so small. Separate the flies by sex and phenotypes. Record the data collected in the table of your lab manual. Experiment one can now perform a new cross with the F1 x F1 with five males and females from the fly house and place them in a new fly house. The testcross is established by wild type females that are heterozygous and dumpy/ sepia males that are homozygous. Lay both houses on its side and clearly label. Experiment two can now perform a F1 xF1 cross with five females and five males from the old fly house into a new fly house. The testcross for experiment two is wild type female’s heterozygous and sepia/ebony males homozygous from the parental generation into a new fly
Variation in selection pressures on the goldenrod gall fly and the competitive interactions of its natural enemies
In order to figure out the genes responsible, there are several other factors that must be determined. These factors include the number of genes involved, if each gene is x-linked or autosomal, if the mutant or wild-type allele for each is dominant, and if genes are linked or on different chromosomes. Proposed crosses include reciprocal crosses between the pure-breeding mutants of strains A and B with the wild-type will help determine if the genes or sex-linked or autosomal, in addition to which alleles are dominant (8). Another proposed cross includes complementation crosses between pure-breading mutants from strains A and B to determine if one or two genes are involved (8). Furthermore, testcrosses between F1 progeny and pure-breeding recessive mutants from strains A and B, which will help determine if genes are linked on the chromosome or if they assort independently (8). These proposed crosses are shown in the attached
17. Fruit flies normally have eight chromosomes. The diagram below shows the result of meiosis in three fruit flies to produce gametes with the number of chromosomes indicated. The male then mates with both female A and female B to produce three zygotes (1, 2, and 3).
Test 4: All three phenotypic frequencies saw a reduction in their number as the homozygote fishes saw a reduction in their number and were not able to pass on their alleles to create either their colored fish or a heterozygote. Both yellow and blue allele frequencies decreased by the same
The idea of the project was to experiment breeding Drosophila Melanogaster (fruit fly) to figure out if certain genes of that species were sex linked or not (autosomal). A mono-hybrid cross and di-hybrid cross was performed. For the mono-hybrid cross, white eyed female and red eyed male were placed in one vial for them to reproduce. For the di-hybrid cross, red eyed and normal winged flies and sepia eyed and vestigial winged flies were placed in their vial to reproduce. In the mono-hybrid cross the results expected were within a 1:1:1:1 ratio. Expected results similar to the expected desired null hypothesis proposed with what the F1 parental generation breeds. The potential results would have had to have been within the ratios of 9:3:3:1. The results were clear and allowed the null hypothesis to be correct. The white eyed gene in the fruit flies is sex linked. Sepia eyes and vestigial wings are not sex linked and are examples of independent assortment.
They are very popular because they have a relatively short life cycle, which lasts about 9 days. This makes it easy to observe multiple generations in a short period of time. The fly’s life cycle consists of egg, larvae, pupa, and adult stages. At each of these stages of development, Drosophila exhibits different phonotypical identities and mutations. The flies that were crossed contained a genetic mutation called apterous. Apterous can be observed by looking for flied that are missing wings. Flies with apterous will not have any wings. If this mutation is sex linked, it is expected that the male population will only be wingless in the F2 generation. If the mutational is autosomal, it is expected that the mutation to normal fly ratio is
Examining the Crosses Between Drosophila Fruit Flies Introduction The major topic of this experiment was to examine two different crosses between Drosophila fruit flies and to determine how many flies of each phenotype were produced. Phenotype refers to an individual’s appearance, where as genotype refers to an individual’s genes. The basic law of genetics that was examined in this lab was formulated by a man often times called the “father of genetics,” Gregor Mendel. He determined that individuals have two alternate forms of a gene, referred to as two alleles.
We then allowed the larvae to hatch, and counted and recorded the total number of flies, the phenotype, and the sex. After taking down all this information this would allow us to perform a F1 cross, we made sure to examine the flies carefully since we needed virgin flies. We prepared a new vial with the a 1:1 ratio of medium and water. After recording the data of the F1 generation, and picking out the virgin flies for the crossing, and we killed of the rest of the flies using the oil method. After some time passed the F1 generation had larva in the vial. Once we noticed the larva we had to put the flies to sleep and collect the data. We then had to prepare another two new vials and medium and water. Carefully observing the flies and picking out three males and three female virgin flies to place into the new vial. Than killing of the other flies. After about a week we had the F2 generation. This was the most important generation, it was what we were looking for to allow us to observe and compare our experiment to Mendel’s experiment. We were looking for a 9:3:3:1 ratio with our flies. Using a basic Punnett square table and the crossing that we had accomplished our results should have looked like the following Punnett square.
One key distinction is the sex combs on the male’s foreleg, which is characteristic that is lacking in the females. Another way to distinguish the sex of the flies is to carefully examine the tip of the abdomen in each. The females tend to have a lighter colored abdomen that is more pointy and long. On the other hand, males tend to have darker pigmented abdomen that is round and short. In general, females tend to have a larger body than males. Sex determination can easily be accomplished even under a low power magnification, which adds to the ease of using fruit flies to study genetic
Anthocyanin is a purple colored pigment in plants that protect tissue from stressful light conditions (Glover and Martin 2012). This experiment utilized monohybrid crosses of the Wisconsin Fast Plant variety of B. rapa with the F_1 generation having one dominant allele for anthocyanin (ANL) and one recessive allele (anl) (Kinds Plants 2014), to complete their life cycle and produce offspring that were observed for their phenotype. By utilizing the genotype of each parent a prediction for the outcome of these genetic crosses was formulated with a Punnett square (Brooker et al. 2014). Since two alleles need to be existent for a recessive trait to be conveyed, and only one allele for a dominant trait (Morgan and Carter 2008), the offspri...
Females like to choose mates who have very bright colors as it is a sign of the male’s good health and vigor. The natural and sexual selection can affect populations in three ways: directional (environmental conditions change in a consistent direction; one extreme of phenotypes is favored), stabilizing (environmental conditions are relatively constant; intermediate phenotype is favored), and disruptive selection (environment has more than one type of useful resource; both extremes of phenotypes are favored). An example of directional selection is pesticide resistance since only the insects with a resistance are favored; an example of stabilizing selection is that the smallest lizards have a difficulty defending their territory whereas the largest lizards are most likely to be eaten by owls; an example of disruptive selection are the black-bellied seedcrackers since they either have a large beak (which they can use to eat had seeds), or a small beak (which allows them to eat soft seeds). Disruptive selection shows a balanced polymorphism, which is when two or more phenotypes are maintained in a
The exercise involved a series of ‘mating’ events resulting in 6 generations. Each mating event produced offspring with ‘possible’ newly inherited traits. The idea of ‘chance’ was included through simple coin tosses. Also, ideas of selection and mutations were introduced into the ‘gene pool’, which presented a deeper and more clear understanding of Mendelian inheritance and the Hardy-Weinberg equilibrium. Upon reaching the third generation, A B1 mutant allele was introduced to the blue locus-influencing fin shape and a G1 mutant allele was introduced to the green locus-influencing Mouth
Flies that began mating on hawthorns now have a population that mate on apples. 2. What is the difference between a.. What is the difference between polygenic inheritance and pleiotropy? Polygenic:
The freshly squeezed citrus juices of Lemon,lime and orange were analysed through titration over the period of 2 weeks. Initially the lime juice was found to be slightly more acidic than the other juices followed by lemon juice and then orange juice since it is known that usually lime is more acidic than lemon and orange. It was hypothesised that the acidity of each juice will increase or decrease after the period of two weeks depending upon the sugar content of that citrus fruit that the juice is extracted from. The acidity of the juice with the highest sugar level will gradually increase over the period of time since sugar contribute to the the fermentation of the citrus juice to lactic acid. The hypothesis was shown to be correct;however
From here, we can see the differences in the target markets of 7-Eleven and the competitors. As mentioned above, 7-Eleven is focused on meeting the needs of convenience-oriented.