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Experiments to investigate factors affecting the rate of diffusion
Investigating diffusion
Diffusion experiment lab report
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When evaluating the results from Investigations 1 and 2, it can be seen that the most influencing factor on the percentage of diffusion was the different surface area of the agar. As predicted in the second hypothesis, the 1cm cube had the highest diffusion percentage with 21.6% of the cube being affected in 8 minutes. This is a significant difference of 18.17% when compared to the 2cm cube’s diffusion percentage of 3.43 and a 20.38% difference from the 2.5cm cube’s result of 1.22%. Investigation 1’s results show that the most successful concentration of acid to diffuse through the phenolphthalein was the 1M with a percentage of 1.81. This is a 0.47% difference from the results of the 2M sulfuric acid. Graph 2 demonstrates that the diffusion percentage continued to incline when the concentration increased however starts to decrease past the 1.8 point. These results were unexpected and so a fifth trial was run in order …show more content…
Overall there were four different methods, two of which can be seen above, each measuring the diffusion of the sulfuric acid. The first method used to measure the impact that influencing factors had on the rate of diffusion was going to be used universally. It focused of cutting the clear agar from the cube and measuring the remaining phenolphthalein to then determine how far the acid diffused into the block. Once testing, this became difficult to obtain exact results and the 20 second intervals between the agars resulted in a busy and cramped work space. For the method Investigation 2, 1cm, 2cm and 3cm blocks were originally planned to be used. However, because majority of the cubes were roughly pre-cut small, the 3cm block was unavailable and so, had to be substituted in for the next largest which was a 2.5cm cube. The third and fourth attempts are the methods that can be referred to in the above investigations and were successful in producing
There were some changes in the amount of solvents and products used. For example, 0.5ml of sulfuric acid was used instead of 20ml.
In conclusion my hypothesis was correct towards this experiment, the concentration with the most Dettol disinfectant had the least bacterial growth and the least Dettol disinfectant had the most bacterial growth occurring in our agar plates, the experiment was successful to show the soil bacterial growth and how well the Dettol disinfectant works to prevent the growth.
has a lower energy state. It will now tend to remain the way it is.
* We would have to leave one end open to fill it up with the different
This experiment was completed with minor deviations from the original protocol. A slight mistake was made when solutions were transported from the test tube to the cuvette. The funnel wasn’t rinsed out completely, and this may have led to slight deviations. However, it may not have been enough to make a significant impact upon the results.
One possible source of experimental error could be not having a solid measurement of magnesium hydroxide nor citric acid. This is because we were told to measure out between 5.6g-5.8g for magnesium hydroxide and 14g-21g for citric acid. If accuracy measures how closely a measured value is to the accepted value and or true value, then accuracy may not have been an aspect that was achieved in this lab. Therefore, not having a solid precise measurement and accurate measurement was another source of experimental error.
Compared that to the reported amount, there was only a 1.3% error, while the class had an average error of 6.35%. These results are quite impressive because the percent errors were quite low for a high school lab. Errors could have occurred during this lab that would have affected the accuracy of the gravimetric analysis. Some errors could include not waiting for a complete filtration of the solution which could have led to a decrease in phosphorous collected or a spill could have occurred. During the last filtration process, it was highly possible that a lost of product could have occurred if the precipitate was able to flow through the filtration paper. In order to prevent this in future investigations, a better filtration paper could be used or the excess water collected in the cup could be refiltered to collect any extra analyte that seeped through. Although the result collected from our group was very close to the reported amount, averaging the result helped other groups who had a high percent error, such as 12%, get a closer mass value and percentage of
Firstly, we need to keep the chemical at a constant concentration. So, in this experiment we have chosen to keep hydrochloric acid at a constant concentration (5cm3). We could have, however, used Sodium Thiosulphate as a constant, but we had chosen to use Hydrochloric acid. Next, we must make sure that the solution is kept at a constant volume throughout the experiment. If the volume is different, then it could give different results if it was at a constant volume.
The Effect of Solute Concentration on the Rate of Osmosis Aim: To test and observe how the concentration gradient between a potato and water & sugar solution will affect the rate of osmosis. Introduction: Osmosis is defined as, diffusion, or net movement, of free water molecules from high to low concentration through a semi-permeable membrane. When a substance, such as sugar (which we will be using in the experiment we are about to analyse), dissolves in water, it attracts free water molecules to itself, and in doing so, stops them from moving freely. The effect of this, is that the concentration of (free) water molecules in that environment goes down. There are less free water molecules, and therefore less water molecules to pass across a semi-permeable membrane, through which sugar molecules and other molecules attached to them are too big to diffuse across with ease.
To determine the suitability of phenolphthalein and screened methyl orange as acid base indicators for detecting the end points of acid-base titrations.
All the tablets were kept in the oven where the temperature was around 70°C for the next day in order to dry them. The tablets with the container were weighted together again after making sure that they were completely dry without moisture in order to determine the erosion percentage by applying the following formula
However, the method of volumetric analysis is highly dependent on a pure standard which the amount of substance present is accurately known. Prior to estimation of the acetic acid, sodium hydroxide (NaOH) was used as the standard solution. Thus, the concentration of the sodium hydroxide (NaOH) was first determined because sodium
Turn on the lamp and as soon as the substance begins to move start timing 10 min. After ten minutes measure in millimeters the distance the solution has
Preparation of Agar containing Petri Dishes: Standard laboratory beaker was thoroughly cleaned and dried before any use. 100ml of tap water was poured into the beaker. The hot plate was switched onto number 1 and left for three minutes to prepare for heating. At the end of three minutes the beaker containing 100 ml of water was placed on hot plate. The water was allowed to heat to 100 °C which would effectively stop any bacterial growth in water. Thermometer was used to measure the temperature of water. 2.3g of Agar powder, measured by using a mass scale, was added to the water while it was being heated. The water was stirred with a rod to make it sure that the agar mixes well and forms a homogenous solution. Once the temperature of the solution rose up to 95°C, the hot plate was slowed down to 0.5 to allow the temperature to rise slowly to 100°C (Fig 3). Once the temperature reached 100°C, the beaker was moved away from the hot plate using a pair of tongs and placed onto the heatproof mat. The next step was to prepare 30 petri dishes which were provided by the lab in packed sterile form. Each petri dish was opened partially
Several factors affect the action of disintegrants such as: ratio of the disintegrant in tablet, particle size, molecular structure, compression force, method of incoroporation, compatibility with other excipients, adding more than one disintegrant, addition of surfactant, tablet hardness the tablets, API nature , mixing, screening and others [5,10,11]. In 1980, Rundic and co-workers found that larger CPV grades (with larger particle size) are more efficient than smaller one [12]. Later in 1981, Smallenbroek et al studied the effect of particle size of the disintegrant on the disintegration of tablet, they found that larger particle size are more efficient than smaller one [13]. Later, Rundic and co-workers studied the effect of crosslinking