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Colorimetric determination
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low originality percentage is indicative of plagiarized papers. Sometimes the score is lower due to long quotations within a document, so please make sure that you use proper citations if this is the case. For more information on our originality scoring process, click here.Colorimetric Techniques
Colorimetry
Colorimetry is the analysis of chemical samples to gather information about their concentration. Colourimetry involves passing light through a sample and measuring how much is absorbed by the solution. Ref -http://www.wisegeek.com/what-is-colorimetry.htm
Most test substances in water are colourless and undetectable to the eye. Therefore to test for their presence I must be able to see them. To avoid sources of error, a colorimeter or spectrophotometer is normally used in colourimetry as both instruments can be used measure the amount of coloured light absorbed by a coloured sample in reference to a colourless sample.-
A colorimetre is an instrument that distinguishes colour samples to provide a measure of the colour’s description. It allows the absorbance of a solution at a certain colour of visual light to be determined. Colorimeters measure change as they determine the concentration of unknown solutions as they are relative to the absorbance.
A colorimetre device operates on Beer-Lambert's law. This law states that the absorption of light transmitted through a medium is directly proportional to the concentration of the medium.
Ref- http://www.wisegeek.org/what-is-a-colorimeter.htm#
A spectrophotometer is a photometer which is a device used for measuring light intensity that can measure intensity as a function of the colour or the wavelength of light. There are a range of different types of spect...
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...nly changed 1 variable which was the unknown solution.
My risk assessment was as detailed as needed to be as I wrote about all the different procedures when using Iron (III) Sulfate, the nature of the hazards, the control measure and what I done to dispose left over Iron (III) Sulfate.
I could modify my experiment to improve it by repeating the experiment 3 times to ensure that I got the most accurate and precise results as I could. I additionally could have compared each of my results to other members within the class as by doing this; it would give me an indication to see if my results were similar. Lastly, another way I could modify my experiment to improve it was to test more concentrations to see if the general trend continues over all of them and by doing this, I could have gotten more precise results from each concentrations transmission and absorbance.
The analyzed yellow#5 wavelength was determined to 395nm because the actual wavelength 427nm was restricted in the Micro lab. The R2 value of the graph is 0.9827, and the level of data accuracy it indicated extremely weak data correlation. The first one dilution data points excluded from the standard curve because the point is not in the linear curve. The first concentration and absorbance value are the highest point in the graph that cannot connect as linear with another data point. After removing the first data point, the standard curve is clear and make
Absorbance was defined as: log I_o/I where I_o is incident light and I is the transmitted light. Fluorescence emission spectrum is different from fluorescence excitation spectrum because it records different wavelengths of chemical s...
Solubility test was used to determine if unknown white compound was soluble in water. To conduct the solubility test, many materials were used such as flask, glass rod, scale, and chemical used was unknown white compound. First, 0.25 gram of unknown white compound carefully measured on scale. Then, the 0.25 gram of unknown white compound added to 100 mL of water and dissolved it using the glass rod. While the unknown
The color that was chose to be shined through the sample was purple. The spectrophotometer was set at a wavelength of 400nm to represent the purple color. It was zeroed using the blank meaning the spectrophotometer read zero as absorbance amount. The blank consisted of 5mL of water and 2.5 mL AVM and it was placed in cuvette. A solution with a known concentration of 2.0x10-4 M was used in the spectrometer. For this solution, 5 mL of the solution with 2.5 mL of AMV was placed in the cuvette. The cuvette was placed inside of spectrophotometer and the amount of absorbance was recorded. This procedure that involves a solution with a known concentration was repeated for the concentrations:1.0x10-4 M,5.0x10-5 M,2.0x10-5M, and1.0x10-5M.A unknown solution absorbance was measured by putting 5 mL of unknown solution with 2.5 mL AMV in a cuvette. The cuvette was placed in the spectrophotometer and the amount of absorbance was recorded. The procedure that deals with the unknown solution was repeated 2 more times with the same solution and the same amount of solution and AMV. The average of the three unknown solution was calculated and the concentration of the unknown solution was
In the second experiment with the green color, I can safely conclude that the color green in this case is very soluble and we would need longer filter paper, perhaps more time to safely separate the different colors that make up the color green.
The LWC Color Guard depends on the capability of its captains and how well they can do their job as a section leader. A leader, in general, is someone who many people look to for advice, instruction, motivation, and most importantly, encouragement. This means that it is important for a leader to have the ability to make difficult decisions and do what is best for the color guard. An essential job of a leader is to guide others as well as being an example of a hard, passionate, and dedicated worker. Some qualities that are necessary for a color guard captain are compassion, character, and courage.
When I dropped iodine to the leaf where I split off from the plant which had waited in red light, its color changed into blue. However, when I dropped iodine to the leaf where I split off from the plant which had waited in green light, its color stayed the same. It is because the plant waited in red light could make more photosynthesis than the other one, so it has more glucose and an iodine solution makes substance’s color blue if it has starch. It is used to test the presence of starch.
The absorbance of these mixtures is measured at a suitable wavelength. If 'x' mole/litre are added to (1-x) mole/litre of M and if C1, C2
0,74 0,87 1,00 0,49 100... ... middle of paper ... ... some groups had got different leakage of the pigment in the test tubes with water.
A cuvette was filled 3/ 4ths of the way and the absorbance measured in a spectrophotometer. The data was compiled as a class and recorded. The Spectrophotometer was blanked using a test tube of distilled water.
SUBTRACTIVE COLOR SYNTHESIS uses paints, dyes, inks, and natural colorants to create color by absorbing some wavelengths of light and reflecting or transmitting others. This subtractive action is the basis of photographic filters, almost all films and color papers, and photomechanical reproduction in color.
ples one through three and a second blank for sample four. A spectrophotometer was set up with a transmittance of 600nm wavelength light for this experiment. Immediately after the cuvettes were properly prepared, they were covered with parafilm and shook vigorously for two seconds to mix the components. The cuvettes were then placed into spectrophotometer and their percent transmittances were recorded. A blank was placed before measuring each cuvette. Table 3 provides the transmittance readings of each samples reduced DPIP.
Photosynthetic pigments are essential for life because they allow photosynthesis to occur by capturing sunlight which is then used alongside carbon dioxide and water to form organic compounds such as glucose and oxygen. The pigments allow the conversion of light energy to chemical energy which other organisms can benefit from. Oxygen is utilised by other organisms in aerobic respiration. The different pigments present in the chloroplasts allow a wide variety of wavelengths of light to be absorbed for efficient photosynthesis and provide colours to the plant to attract pollinators.
Spectroscopy Spectroscopy is the study of energy levels in atoms or molecules, using absorbed or emitted electromagnetic radiation. There are many categories of spectroscopy eg. Atomic and infrared spectroscopy, which have numerous uses and are essential in the world of science. When investigating spectroscopy four parameters have to be considered; spectral range, spectral bandwidth, spectral sampling and signal-to-noise ratio, as they describe the capability of a spectrometer. In the world of spectroscopy there are many employment and educational opportunities as the interest in spectroscopy and related products is increasing.
Hue is the common name for the colours in the spectrum which are red, orange, yellow, green, blue, and violet. A pigment is a colouring ag...