I.Introduction When future generations reflect upon this current era of human history and development, it is almost certain that the birth and advancement of bioinformatics will be among the most awe-inspiring of topics. Bioinformatics is the field of study which is concerned with the storage, retrieval, and analysis of biological data via the tools of informatics. The two major objectives of bioinformatics (as identified by -new avenues-p9) are the identification of genes and the prediction of their function. Bioinformatics therefore shares an intimate relationship with the field of functional genomics, which is defined by Alberts as “the field of study which aims to understand the ways in which cells execute and control the great number of operations required for normal function, and those in which cellular systems fail in disease”(1996). In order to produce results that offer a better understanding of biological systems, a link must be made between the genomic scale and the scale of the biological system in question. This requires computational and formal methods to process massive amounts of data, understand general principles that govern the system, and make predictions about system behavior (Scmulevich,Dougherty, and Zhang,2002). As the size and complexity of the data to be implemented grows telescopically (due to advancements in areas such as gene expression, mass spectrometry, and protein-protein interactions), the need for massive comprehensive databases with fluid global access (deemed “knowledgebases” by Manuela Pruess (p43,New Avenues.)) has become apparent. What separates these new proposed knowledgebases from other types of databases is the power which stems from the cross-referencing of data from researchers all... ... middle of paper ... ...f Non-governmental organizations is possible. These include the success of Greenpeace in forcing the company Shell Oil to change its position in Brent Spare and the North Sea in respect to Nigeria and the success of human rights groups in forcing the government of Mexico to protect the Indians in Chiapas, the instance social pressure discontinuing the production of the “new leaf” genetically modified potato, and the success of human rights groups in changing the Nike position on child labor use. While these instances have proven that NGOs are capable and viable resources in altering public policy of large companies and governments, the reliance of NGOs alone to regulate the advancement of the biotechnology industry offers a fundamental problem in that they lack the legitimacy to create organizations which monitor and enforce the policies which they help to create.
It helps medics to find a direct genetic cause of the patient’s condition and target it with pharmaceutical or other therapies. The technology is used for the identification of DNA sequences that increase risks of current diseases and disorders; with this information carriers can start to make efforts to prevent them before the development of the problem. The video mentioned 200 actionable genes, structures that have direct links with a specific condition. Knowing about their presence, people have a chance to bring in preventive measures like taking anticoagulants in the case of identification of a thrombogenic gene. The technology led to the significant improvement of diagnostics and personalized treatments. It helped to find a rare, life-threatening mutation in case of Beery twins and assign a drug to a girl (Alexis) that returned her to a normal life. In the case of cancer genome sequencing led to the development of genetic drags, which target essential tumor genes and make malign structures to shrink. The video mentioned a product that works with the BRАF protein that induces cells to uncontrolled division; the drug led to the remission in the patient with metastasizing melanoma. Such treatment was effective in the case of cystic fibrosis. In the case of the breast cancer the technology helps to evaluate the aggressiveness of the condition and make a personalized decision about chemotherapy. The video also mentioned the pre-implantation genetic diagnosis – an early-staged technology that prevents the development of inherited disorders in
Firstly, an amount of 40.90 g of NaCl was weighed using electronic balance (Adventurer™, Ohaus) and later was placed in a 500 ml beaker. Then, 6.05 g of Tris base, followed by 10.00 g of CTAB and 3.70 g of EDTA were added into the beaker. After that, 400 ml of sterilized distilled water, sdH2O was poured into the beaker to dissolve the substances. Then, the solution was stirred using the magnetic stirrer until the solution become crystal clear for about 3 hours on a hotplate stirrer (Lab Tech® LMS-1003). After the solution become clear, it was cool down to room temperature. Later, the solution was poured into 500 ml sterilized bottle. The bottle then was fully wrapped with aluminium foil to avoid from light. Next, 1 mL of 2-mercaptoethanol-β-mercapto was added into fully covered bottle. Lastly, the volume of the solution in the bottle was added with sdH2O until it reaches 500 ml. The bottle was labelled accordingly and was stored on chemical working bench.
The Board is responsible for enforcing biological patents on Bt Cotton and reassuring through investigations that farmers are not defying their legal jurisdictions. Defiance of laws would result in litigation carried out by the board. Their monopolization in the Indian agricultural market bears power, which can be utilized to unjustly influence the Indian government. Their enforcements of biological patents yields them with great legitimacy. They are entitled to urgency as the effect of their patents on Indian farmers should be called to immediate attention, as it results in catastrophic impacts on human lives. The Board of Directors can be classified as a supportive stakeholder as their enforcement of patents maximizes revenue, which is acting in the best interest of Monsanto as a whole. The Board meets all the requirements of “Mitchell, Agle, and Wood’s Theory of Stakeholder Identification and
In 1990, the first great stride of genetics took place. This was called the Human Genome Project, a large-scale operation that was designed to understand the human genome (genetic structure). Since its commencement, there have been many leaps and bounds that have taken place. For certain genetic issues that we once knew nothing about, we no...
Proteogenomics is a kind of science field that includes proteomics and genomics. Proteomic consists of protein sequence information and genomic consists of genome sequence information. It is used to annotate whole genome and protein coding genes. Proteomic data provides genome analysis by showing genome annotation and using of peptides that is gained from expressed proteins and it can be used to correct coding regions.Identities of protein coding regions in terms of function and sequence is more important than nucleotide sequences because protein coding genes have more function in a cell than other nucleotide sequences. Genome annotation process includes all experimental and computational stages.These stages can be identification of a gene ,function and structure of a gene and coding region locations.To carry out these processes, ab initio gene prediction methods can be used to predict exon and splice sites. Annotation of protein coding genes is very time consuming process ,therefore gene prediction methods are used for genome annotations. Some web site programs provides these genome annotations such as NCBI and Ensembl. These tools shows sequenced genomes and gives more accurate gene annotations. However, these tools may not explain the presence of a protein. Main idea of proteogenomic methods is to identify peptides in samples by using these tools and also with the help of mass spectrometry.Mass spectrometry searches translation of genome sequences rather than protein database searching. This method also annotate protein protein interactions.MS/MS data searching against translation of genome can determine and identify peptide sequences.Thus genome data can be understood by using genomic and transcriptomic information with this proteogenomic methods and tools. Many of proteomic information can be achieved by gene prediction algorithms, cDNA sequences and comparative genomics. Large proteomic datasets can be gained by peptide mass spectrophotometry for proteogenomics because it uses proteomic data to annotate genome. If there is genome sequence data for an organism or closely related genomes are present,proteogenomic tools can be used. Gained proteogenomic data provides comparing of these data between many related species and shows homology relationships among many species proteins to make annotations with high accuracy.From these studies, proteogenomic data demonstrates frame shifts regions, gene start sites and exon and intron boundaries , alternative splicing sites and its detection , proteolytic sites that is found in proteins, prediction of genes and post translational modification sites for protein.
Everyone has likely already heard the name Monsanto, the largest seed distributor in the world. Nearly fifty percent of all seeds in the world are owned by only three corporations. These companies account for trillions in fertilizer, pesticide, seed patents and herbicide sales annually. These companies have been building a seed empire within the food industry for over thirty years now and they have plans to patent every seed with biotechnology. Thoreau wrote in his book, “The condition of the operatives is becoming every day more like that of the English; and it cannot be wondered at, since, as far as I have heard or observed, the principal object is, not that mankind may be well and honestly clad, but, unquestionably, that the corporations
Having completed the Human Genome Project, scientists now seek to uncover the secrets of the human proteome (Begley 1). It is "guesstimated" that the proteome, meaning all the proteins, will involve up to 1000 times more data than the genome did. But this again brings us to the question: What will the scientific and medical communities do with all this information?
Falkner, Robert. “The Global Biotech Food Fight: Why The United States Got It So Wrong.” Brown Journal Of World Affairs 14.1 (2007): 99-110. Academic Search Premier. Web. 2 Nov. 2011.
Information molecules are molecules that all life forms, from viruses to humans, use for the passage of genetic information from one generation to the next. Information molecules are also provide the instructions for cell functions [1]. On Earth, two types of information molecules are used. There are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is a double-helical structure that is transcribed by RNA and in turn translated into proteins. Protein molecules carry out all cellular functions. This is central dogma of biology on Earth. However, synthetically made PNAs have shown that DNA and RNA are not the only information molecules that can exist.
According to Galyna Sidyelyeva, “research has provided insight into the issue of the nutritional value of GM crops. For example, the Venneria group tested genetically modified wheat, corn, and tomatoes. They found wheat and corn are similar to their non-GM counterparts and tomatoes differ only in their antioxidant content, having a lower amount than their unmodified relatives” (50). With this in mind there are many people in the world today that may find value from GM crops; for example, in countries where people are starving. While the crops may not be identical to its non-GM counterparts, these countries will still profit from them. In “A dubious success: The NGO campaign against GMOs,” Robert Paarlberg asserts, “Unfortunately, in the case of NGO [non-GMO] campaigns against GMO crops, they typically emerge from rich countries while imposing consequences on poor countries” (228). As one can see, these rich countries may have succeeded in not allowing some GMOs in but to date in America they still are not labeled; so, they are only hurting the poor countries by trying to make a controversy over labeling that is
Distinct characteristics are not only an end result of the DNA sequence but also of the cell’s internal system of expression orchestrated by different proteins and RNAs present at a given time. DNA encodes for many possible characteristics, but different types of RNA aided by specialized proteins sometimes with external signals express the needed genes. Control of gene expression is of vital importance for an eukaryote’s survival such as the ability of switching genes on/off in accordance with the changes in the environment (Campbell and Reece, 2008). Of a cell’s entire genome, only 15% will be expressed, and in multicellular organisms the genes active will vary according to their specialization. (Fletcher, Ivor & Winter, 2007).
I have always been fascinated by Biology and Computer Science which propelled me to take up my undergraduate studies in the field of Bioinformatics. As a part of my undergraduate curriculum, I have been exposed to a variety of subjects such as “Introduction to Algorithms”, “System Biology”, “PERL for Bioinformatics”, “Python”, “Structure and Molecular Modeling” and “Genomics and Proteomics” which had invoked my interest in areas such as docking algorithms, protein structure prediction, practical aspects of setting and running simulation, gene expression prediction through computational analysis. These fields have both a strong computational flavour as well as the potential for research which is what attracts me towards them.
Developed countries, like the United States, must face the consequences of presenting GM crops to Third World countries and must offer to solve any problems with honest and just solutions. Developed countries can then truly say that they are responsible leaders of the world. Works Cited Anderson, Clifton. The "Genetic Engineering" The Futurist. The.
Cheminformatics term was coined for the first time by F.K. Brown and it's defined as "the field of chemistry that integrates chemical data with analytic and molecular design tools finding the 'best- fitting' compounds to address particular targets". It can be called also "chemoinformatics", "chemioinformatics" or "chemical informatics". In silico techniques are used in cheminformatics for a wide range of applications, such as in rotational drug design or in drug diversity, using the structure for predication of the activity and in virtual screening. This was first applied in the making of the period table
Since school days the terms like cell, genes, DNA, protein intrigued me a lot and helped me in realizing the dream of pursuing a career in Biotechnology field that has been making many remarkable achievements. The passion towards biological sciences made me to take B.TECH in Biotechnology in Dr. M.G.R. University.