laboratory manual as well as my class notes and power point print outs. I was given an incubated agar slant labeled #16 and a rack of different tests to either examine or perform myself; the tests are as follows: Gram Stain; Nutrient Gelatin Test; Carbohydrate Fermentation; Dextrose, Lactose and Sucrose; IMVIC tests; Citrate, Indole, Mythel-Red and Vogues Proskauer test; as well as a Urease and TSI Test. Materials and Methods/Results Upon receiving the unknown Microorganism (M.O.) #16, I prepared a
In Emily Dickinson’s lyrical poem “There’s a certain slant of light” she describes a revelation that is experienced on cold “winter afternoons.” Further she goes to say that this revelation of self “oppresses, like the Heft of Cathedral Tunes” and causes “Heavenly Hurt”, yet does not scare for it is neither exterior nor permanent. This only leaves it to be an internal feeling, and according to Dickinson that is where all the “Meanings” lie. There’s no way for this feeling to be explained, all that
as the other plates. After a close observation of the EMB it was more possible and useful to test the plate for isolation. The next biochemical test for Entries required being examed by the medium of the Triple sugar Iron Agar test as known as the TSI. The tube would streak and stab at the center and will be incubated for the next 48 hours. The gram positive was observed with the PE Blood agar plate. The plate had a creamy white growth on the red blood agar. First the there was a smear on a slide
In microbiology, bacterial identification relies on isolating individual bacterial colonies of different species. The streaking for isolation technique is a tool used to obtaining a pure culture. The technique consists of taking an original inoculum that contains a mixed culture and spreading the bacteria into four quadrants on a solid medium. The purpose of spreading the mixed culture on a petri dish is to reduce the number of bacteria in each subsequent quadrant to one parent cell of each type
that I had to redo some test all over again just to make sure. I even had to streak a plate again because of the spring break vacation, probably the bacteria died during that time and also there was contamination on them. Even though I had my TSIA slant backup I didn’t have that much bacteria to work on since I had to keep repeating some of the test. After gathering my result of each biochemical test and eliminating organism that wasn’t my unknown with the given result I still kept second guessing
Rat Bite Fever is an infectious disease that is caused by two different strands of bacteria. Streptobacillary Rate Bite Fever is caused by Streptobacillus moniliformis and is found primarily in the United States. Spirillary Rat Bite Fever is caused by Spirillum minus and is mostly found in Asia. Most people get Rat Bite Fever from infected rodents or contaminated food or water and if not treated, the infection can become very severe or fatal. In this paper, I will discuss the types of Rat Bite