Isolation and Characterization of Eugenol from Cloves Jean Theresse Perez Human Biology 22 De La Salle University-Dasmariñas Dasmariñas City, Cavite, Philippines ABSTRACT Eugenol was extracted from cloves through the process called steam distillation. A total of 50ml of distillate were collected with temperature monitoring for every 5.0ml of collected liquid. The distillation curve showed that the temperature of volatilization of eugenol is from 98°C to 99°C which is way below its boiling point at 2540C. Chemically active extraction was conducted in order to remove impurities particularly eugenol acetate and caryophyllene. This involves the addition of several solvents particularly hexane, NaOH, HCl, then hexane again in succession. …show more content…
The sample was subjected to steam distillation as illustrated in Figure 1. A total of 50ml of distillate was collected while recording the temperature for every 5.0 ml of distillate. The distillate was transferred into a 250ml Erlenmeyer flask and 3.0 g of NaCl was added. The flask was cooled and the content was transferred into a 250-ml separatory funnel. Then 25.0ml of hexane was added and the mixture was shaken for 5 minutes with occasional venting. The aqueous layer was discarded and the organic layer was left inside. About 25.0ml of 10% NaOH was then added and the mixture was shaken as before. The aqueous layer was collected and then cooled in an ice bath. It was then acidified with enough 6.00 M HCl while the pH is being monitored with red litmus paper. Another 25.0 ml of hexane was added and the mixture was shaken as before. The hexane extract was saved and a small amount of anhydrous sodium sulfate was added. The mixture was then swirled for a couple of minutes then filtered. A small amount of the final extracted was tested separately with 1% FeCl3 and Bayer’s reagent. …show more content…
Initially, NaCl was added in order to demulsify the distillate. Next is the addition of NaOH which solubilise the acidic eugenol into a phenoxide salt, leaving the other components that are neutral in nature. Addition of HCl to the base neutralizes the phenoxide to reform the original acidic form of eugenol. Hexane then solubilises eugenol from the aqueous layer. Anhydrous sodium sulphate was added in order to remove the remaining water in the organic
Saturated sodium chloride solution, also known as brine solution, is used to wash the distillate mixture. The distillate mixture is the phosphoric acid the co-distilled with the product. The brine solution also removes most of the water from the 4-methylcyclohexane layer. When six drops of 4-methylcyclohexene were added with two
Introduction In this experiment, steam distillation was used to isolate eugenol oil from cloves. The goal of this experiment was to test and analyze the purity level of the eugenol oil that was isolated by applying a TLC test as well as H-NMR spectra. At the end of this experiment, eugenol oil was isolated, but some errors that occurred during the experiment resulted in impurities in the final isolated oil.
Once the mixture had been completely dissolved, the solution was transferred to a separatory funnel. The solution was then extracted twice using 5.0 mL of 1 M
The objective of this experiment was to perform extraction. This is a separation and purification technique, based on different solubility of compounds in immiscible solvent mixtures. Extraction is conducted by shaking the solution with the solvent, until two layers are formed. One layer can then be separated from the other. If the separation does not happen in one try, multiple attempts may be needed.
Performing this experiment, we used the technique called Acid-Base extraction to isolate Eugenol, which is one of the main ingredients of clove oil. Acid-Base extraction is the most efficient method for isolating organic component; it is efficient because it purifies the acid and base mixture based on their chemical identities. We have seen throughout this experiment that acid and base play an important role, when it comes to solubility in water. Our basic knowledge of acid and base is acid is a proton donor and base is a proton acceptor. This ideology helps us to understand why organic compounds are not soluble in water. When compounds tend to be insoluble, we have to use acid and base reaction, to change its solubility. The changes that occurred
In a fume cupboard using a graduated pipette, 3 mL of pyridine was added to the conical flask, followed by 0.41 mL of benzoyl chloride. The solid produced in the conical flask was yellow and had a creamy texture. The mixture was then heated on a steam bath for 10 minutes and then cooled in a beaker of ice-cold water to improve recovery and ensure full recrystallisation. A 15 mL aliquot of methanol was then added to the mixture and the solid product collected under suction filtration in a Büchner funnel. The solid was then washed with two 20 mL aliquots of methanol and then dried again under suction filtration.
Materials and Methods: An ion exchange chromatography column was obtained and set up for purification with the addition of 0.5 ml ion exchange matrix. 1 ml
After corking and inverting the reaction tube, the organic layer was removed and transferred to another reaction tube. Then another increment of 0.5 mL of methylene chloride was added to the first reaction tube and the extraction process was repeated. This was done a total of three times. Then in step thirteen, about five scoops of anhydrous sodium sulfate was added to the second reaction tube. Then a filter pipette was prepared and the organic solution from the second reaction tube was added to the pipette.The mixture was allowed to gravity filter and an additional 0.5 mL increment of methylene chloride was added into the pipette and the process was repeated again. Then in step 18, the collected solutions poured onto a watch glass. It was observed that the pre-weighed watch glass weighed 26.196 grams. The watch glass was placed over the beaker that was filled with 30mL of water. It was observed that it took about fifteen minutes for the solvent
In this experiment, lipids from ground nutmeg are extracted using a combination of solvents and identify the lipids through chromatography. The purpose of using solvent combinations is to elute the lipids based on their polarity to binding of the silica gel. The chromatography is performed on a silica gel plate and the use of iodine to visualize the lipids. By calculating the Rf values for each compound and comparing them to the known lipids, we are able to distinguish the lipids within the grounded nutmeg.
The materials used during Part 2 of the experiment includes an unknown metal (either A, B, or E), an analytical balance 200 mL of tap water, a 400 mL beaker, a hot plate, 50.0 mL of cold water, and a calorimeter. The materials used during Part 3 of the experiment includes 50.0 mL of deionized water, 2 g of NH4Cl, an analytical balance and a
...ve lipid profiles (arising from thyroid-glands) reduces cholesterol and prevent from oxidation. De-oxidation or reduction of cholesterol results in breaking the large chain of molecules of fats clotted in venal tracks broken into smaller parts leaving the venal track. As a result the risk of heart attack and atherosclerosis is minimized or controlled. As it has a toxic level up to some strength. Clove is useful in killing parasites. These parasites could damage the tissues of heart, lungs or kidneys, creating painful diseases like cancer.
In this experiment three different equations were used and they are the Stoichiometry of Titration Reaction, Converting mL to L, and Calculating the Molarity of NaOH and HCl (Lab Guide pg. 142 and 143).
The dried chicory roots are roasted, grounded and then mixed with water. After allowing the mixture to stand for few hours, the insoluble part is separated using filtration and centrifugation. The liquid extract thus obtained is further refined to make it suitable as a food additive. Liquid chicory is packed in 290 litre barrels for easy transport. The liquid chicory extract contains 98% of insulin by weight. Some of the other applications of liquid chicory are due to its anti-inflammatory characteristics and ability to purify the human blood. Chicory liquid form is also used as mild sedative as well as alterative in the medical world. It can also be used in variety of medications like stomach cleaner and gallbladder tonic.
Zinc eugenate may decompose in the presence of water through the release of eugenol, making it weak ...
Clove oil was extracted from the buds of syzygium aromaticum by using hot extraction method with the help of Clevenger apparatus . clove was purchased from the market in jalndhar (Punjab). And the clove was then filled inside round bottom flask and this flask was fitted with Clevenger then putted it on heating mantle. We use water as solvent and heatd for 1.5 to 2.5 hours at a temperature around 60 degree celcius and clove oil comes in the narrow tube of the apparatus from there we collected it through tap. We dry the extract with the help of sodium sulphate (Na2so4).