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Identifyingthe white substance lab report
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The purpose of the Unknown White Compound Lab was to identify the unknown compound by performing several experiments. Conducting a solubility test, flame test, pH paper test, ion test, pH probe test, conductivity probe test, and synthesizing the compound will accurately identified the unknown compound. In order to narrow down the possible compounds, the solubility test was used to determine that the compound was soluble in water. Next, the flame test was used to compare the unknown compound to other known compounds such as potassium chloride, sodium chloride, and calcium carbonate. The flame test concluded that the cation in the unknown compound was potassium. Following, pH paper was used to determine the compound to be neutral and slightly
Solid A was identified to be sodium chloride, solid B was identified to be sucrose, and Solid C was identified to be corn starch. Within the Information Chart – Mystery White Solid Lab there are results that distinguishes itself from the other 4 experimental results within each test. Such as: the high conductivity and high melting point of sodium chloride, and the iodine reaction of corn starch. Solid A is an ionic compound due to its high melting point and high electrical conductivity (7), within the Information Chart – Mystery White Solid Lab there is only one ionic compound which is sodium chloride, with the test results of Solid A, it can be concluded that is a sodium chloride. Solid B was identified as sucrose due to its low electrical
1 / 3 BIO3001 Shinhye Jeon (Heather) Professor Wahlert November 21, 2017 Identifying of Spores Belonging to the Division Pterophyta by Utilizing Phylogenetical method 1. Abstract
The technique used to narrow down the identity of unknown white compound were solubility test, pH test, flame test, and ion test. The first technique used to narrowed the properties of unknown white compound was using solubility test. To conduct solubility test, 0.25 gram of unknown white compound was dissolved in 100 mL of water. After carefully observing the change while string unknown white compound in water, the unknown white compound was soluble because it dissolved in water completely. Using bursen burner, matches and deionized water, flame test was conducted for unknown compound and it burned lilac color. Then compared the color of unknown white compound to other compound that were narrowed. The results of flame test for compounds that were narrowed down is shown in the following table. The pH test was conducted using litmus paper. 0.50 gram of unknown white compound was measured and dissolved in 10 mL of water in beaker. After dissolving, placed the litmus paper in solution and recorded the pH value of unknown compound which was neutral. Then compared the pH value of unknown white compound to compound that were narrow down. The pH result of the KCL, KNO3, NaCl, and K2SO4 is presented in the following table. The ion test was also conducted in order to make sure that the identity of unknown white compound was matched with the compound that were narrow down. After conducting the test, the result of unknown white compound which formed precipitate compared to KCl, KNO3,NaCl, and K2SO4. The results shown in the following
The purpose of this laboratory is to learn about cultural, morphological, and biochemical characteristics that are used in identifying bacterial isolates. Besides identifying the unknown culture, students also gain an understanding of the process of identification and the techniques and theory behind the process. Experiments such as gram stain, negative stain, endospore and other important tests in identifying unknown bacteria are performed. Various chemical tests were done and the results were carefully determined to identify the unknown bacteria. First session of lab started of by the selection of an unknown bacterium then inoculations of 2 tryptic soy gar (TSA) slants, 1 nutrient broth (TSB), 1 nutrient gelatin deep, 1 motility
The gas chromatography calculations offer the area values for under the peak curves. Those area values are directly correlated with the relative concentrations of each alkene product in the mixture. When the ratio of the two values is calculated, 3-methylcyclohexene being the 2nd peak area value and 1-methylcyclohexene being the 3rd peak area (represented on table 1), (11152:1283) = 8.7 1-methylcyclohexene molecules: 1 3-methylcyclohexene molecule.
Many steps were taken in order to narrow down and figure out which bacterium unknown 413 is. One of the first step taken in identifying unknown 413 was to do a gram stain. The gram stain, which was purple, resulted in gram-positive cocci. The gram-positive eliminated all the possibilities of unknown 413 being any gram-negative bacteria such as Escherichia coli, Enterococcus faecalis etc. The cocci morphology result eliminated any possibilities of it being any bacteria that are a rod. This left five different genera: Staphylococcus, Lactococcus, Micrococcus, and Enterococcus as a choice for the unknown.
Luminol is a white, sometimes pale yellow, crystalline solid that can create a chemiluminescence when mixed with certain oxidizing agents. In order to create luminol, there needs to be a diamindation of 3-Nitrophthalic Acid by Hydrazine, which produces 3-Nitrophthalihydrazine and is then reduced with Sodium Dithionite. The product on its own is not soluble in water but is soluble in most organic solvents. Other than emitting a blue light, luminol is also used in forensic studies. A forensic investigator can use luminol to detect traces of blood by spraying it on an area since it traces an activation oxidant in order to make it emit light. In order to create effective results, investigators must spray even amounts of the solution. The intensity
In this report, two unknown metals are given and using the materials required for this experiment, the unknown metals must be identified. The two metals are labeled as unknown metal A and B. The procedure informed the audience that one metal is Tin and the other is Aluminium. Knowing the physical properties of a metal, it came to a hypothesis that unknown metal A was Tin and unknown metal B was Aluminium. The reason to the hypothesis is unknown metal B had a shiny luster to it and had the same color as aluminum foil used for food. Therefore, unknown metal B is aluminum making unknown metal A tin.
Gel filtration is one of the many methods that can be used for purifying proteins. Gel filtration, also known as size-exclusion chromatography, separates proteins based on size. There are pros and cons for this methods. One of the pros is that fragile proteins will not or hardly get damaged when going through the chromatography column. One of the cons is the results are not very accurate due to the proteins’ inability to adhere to the column at times.
Mold is a member of the fungi family. Since mold is part of the fungi family, it cannot use the sun to obtain energy. This means that mold has to use other plants or animals to grow. Even though they cannot see them, there are millions of mold spores in the air. These spores settle down and start to multiply which can be done rapidly or slowly as long as it has a food source. Mold usually grows best in warm environments, but it can still grow in cold environments also. Mold can cause illness such as vomiting or feeling nauseated when it is eaten or when it smells bad.
The efflorescence and deliquescence was determined for all the compounds. Efflorescence is the tendency of a compound to lose water to the air while deliquescence is the tendency of a compound to gain water from the air. KAl(SO4)2 ∙ 12 H2O was classified as an efflorescent compound because it lost mass after being opened to the air. The other compounds, CaCl2, CuSO4, and CoCl2, were all deliquescent compounds and gained mass after being opened to the air.
In this experiment, the chromatography of skittles and Crayola markers will be used to determine how the dyes that are used in the markers and candy of the same color compare to each other. By finding the chromatography of these substances, we will be able to determine whether the dyes used in these materials are the same or if they differ. We will also be able to determine if the dyes used in Crayola markers are safe to digest. My hypothesis is that the dyes used in Crayola markers and skittles of the same color will differ, but the dyes used in Crayola markers are not toxic. To better support this hypothesis, the process of chromatography and the toxicity of various dyes will need to be explained.
The purpose of this lab was to analyse how light and color is created inside an atom in order to deduce what an unknown chemical compound was. We did two different experiments in order to examine how light is created and changed. In one, we looked at how chemical compounds affected a flame’s color, and to determine what the unknown salt contains. In the other lab, we looked at how atoms create light, and how it is displayed on the elements individual spectral line.
In my morning I helped Adriana catch a voided urine sample for her on a male Rottweiler. I previously discussed how I caught a voided urine sample in journal four.
The effects of temperature on the distribution of organic contaminants between different phases in the subsurface soil was demonstrated by Davis 1997. Calculations were carried out using the data of Heron et al. (1996) for tri-chloro ethane (TCE) at 90°C. The results indicated that raising the temperature to 90°C caused significant increase in the concentration of contaminant in the air phase under both high and low soil organic matter conditions and significantly decreased the amount that is associated with the solids. Only small amounts remain in the liquid phase. The researchers concluded that if the high organic matter content soil is under water saturated conditions, the amount of TCE in the water would approximately double as the temperature increased from 20°C to 90°C, but 82 percent of the TCE would remain adsorbed to the solids. Under low organic matter and water saturated conditions, there would be approximately a 30 percent increase in the amount of TCE in the water phase with an increase in temperature from 20°C to 90°C, leaving approximately 25 percent