The Concentration of Sugar in the Cell Sap of a Potato Cell's Vacuole Plan
In this experiment I am intending to investigate the concentration of
sugar in the cell sap of a potato-cell's vacuole. I will discover this
using my knowledge of the biological process of Osmosis.
Osmosis is the movement of solvent (usually water) through a
semi-permeable membrane separating solutions of different
concentrations. The solvent passes from a more concentrated solution
to a less concentrated solution until the two concentrations are
equal.
Many cell membranes behave as semi-permeable membranes, and osmosis is
a vital mechanism in the transport of fluids in all living organisms,
the transport of water from the roots up the stems of plants is just
one of these.
[IMAGE]
Apparatus
[IMAGE]For this experiment I will need the following apparatus:
1. One large potato,
2. [IMAGE]Five glass beakers,
3. One measuring cylinder (50ml),
4. One potato corer,
5. [IMAGE]One dissection tile,
6. One scalpel,
7. One measuring scale (2dp)
8. [IMAGE]Ruler (1dp).
To discover the concentration of sugar in the cell sap I will place a
piece of potato, exactly 2.5cm in length, in a beaker of salt solution
(varying in strength) and leave it for 24 hours. If the piece of
potato increases in weight, the concentration of water in the solution
is too low, therein, if it decreases in weight the concentration of
water in the solution is too high. But if there is no change in weight
whatsoever then the concentration of water in the solution ...
... middle of paper ...
... weight because there is a high difference between the concentration of
water inside the cell and the concentration outside the cell.
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I believe the potato will greatly decrease in weight because water is
now at a much higher concentration inside the cell than outside.
Therefore, due to Osmosis, the water molecules will move from where
they are in high concentration to where they are in low concentration:
leaving the cells and entering the solution.
Collection of Results
To be sure that my results are collected and recorded in the correct
manner, one which is easy to both understand and compare, I have
constructed four results tables which can be seen overleaf. I have
crated a separate table for every experiment and one in which I can
record the average result of each aspect of those experiments.
This is because there are more water molecules in a given volume to react with the Alka-Seltzer tablets. However if there are sufficient water molecules to occupy the Alka-Seltzer tablets an increase in the water molecules will not alter the rate of the reaction. This is because the Alka-Seltzer tablets are already working as fast as they can to react with the water. The concentration can be increased by increasing the volume of the water used in the reaction to dissolve the Alka-Seltzer tablets in. To control the... ...
If we say that the right hand side in picture 1 is the potato, and the
Investigation of the Concentration and the Effect of Sucrose on Osmosis in Apple and Potato Tissues
Moisture is heavy, and thus it can change the results of the experiment, as we only want the weight of magnesium and the magnesium oxide.
Purpose: This lab gives the idea about the enzyme. We will do two different experiments. Enzyme is a protein that made of strings of amino acids and it is helping to produce chemical reactions in the quickest way. In the first experiment, we are testing water, sucrose solution, salt solution, and hydrogen peroxide to see which can increase the bubbles. So we can understand that enzyme producing chemical reactions in the speed. In the second experiment, we are using temperature of room, boiling water, refrigerator, and freezer to see what will effect the enzyme.
The Effects of Concentration of Sugar on the Respiration Rate of Yeast Investigating the effect of concentration of sugar on the respiration rate of yeast We did an investigation to find how different concentrations of sugar effect the respiration rate of yeast and which type of concentration works best. Respiration is not breathing in and out; it is the breakdown of glucose to make energy using oxygen. Every living cell in every living organism uses respiration to make energy all the time. Plants respire (as well as photosynthesise) to release energy for growth, active uptake, etc…. They can also respire anaerobically (without oxygen) to produce ethanol and carbon dioxide as by-products.
Osmosis in Potato Tubes Osmosis: Osmosis is the movement of water molecules through a semi-permeable membrane from a high concentration to a low concentration. Diagram: [IMAGE] [IMAGE] Aim: To see the effects of different concentration of sugar solution on Osmosis in potato tubes. Key factor: In the investigation we change the sugar solution from: 0%-10%-20%-30%-40%-50% this is the independent variable; the dependant variable is the change in mass. Prediction: I predict that all the potato tubes in pure water or low concentration sugar solution will swell because water enters their cells by osmosis.
* Note the mass down in the table at the end of the first page.
This is because the water molecules passed from a high concentration in the water, to a low concentration, in the piece of potato. Therefore, the potato in higher water concentrations will have a larger mass than in higher sucrose concentrations. i.e. The potato pieces are shown to have water potential. The attached graph shows the concentration of the sucrose solution plotted against the average mean change in mass.
the same way as it does potato. I would also widen the range of sugar
However, the solution could be more water than salt so the water from the solution could be more concentrated than the water inside the potato cells. If so then In theory the water in the solution should diffuse into the potato. cells and increase in mass. Apparatus: Potato to perform the experiment on. Thin metal tube to cut potato chops with.
4. Put each group of potato discs in one of the 6 test tubes and watch
Prediction I think that when the potato is placed in distilled water the potato mass will increase. This is because water, has, if pure, a weaker concentration than the potato, and the water molecules move in through a partially permeable membrane by osmosis. If the potato is in a high concentration salt solution, the potato mass will decrease because the potato is less concentrated than the salt solution, and the water will move through the partially permeable membrane into the stronger solution. However, if the potato mass stays the same after the experiment, this means that the water/salt solution and the potato must be of equal concentration.
there would be no flow of water into or out of the cell so the cell
second test tube also add 6 mL of 0.1M HCl. Make a solution of 0.165