The Action of an Enzyme
Introduction
The breakdown of wastes in liver cells produces hydrogen peroxide
which is poisonous. This must be removed if the cell is to remain
unharmed.
Method
1. Label the test tubes A, B, C, and D. label the staining dishes B,
C, and D.
2. Put 5cm3 of hydrogen peroxide in each test tube. Be careful
-hydrogen peroxide is dangerous.
3. Put two cubes of raw liver into another test tube and put this in
the water bath (set at boiling) for 2 minutes.
4. Use forceps to remove the cubes from the test tube. Place one cube
on the staining dish B. Place the second cube in the mortar.
5. Grind the boiled cube (add a little water if necessary). Put the
paste on staining dish C (use a glass rod if necessary). Wash the
pestle and mortar.
6. Grind one raw cube in the mortar. Put the paste on staining dish D.
7. Put the remaining, uncooked cube of liver in test tube A; the
boiled cube in B: the boiled liver paste C and the raw liver paste in
D. Work quickly so that the liver is added to each tube more or less
at the same time. Start the stop clock at once.
8. After a suitable time interval, measure in centimeters and record
the height of the froth in each tube.
Results
Tube treatment of liver height of froth (cm)
A raw solid cube 10.5cm
B boiled solid cube 11cm
C boiled ground paste 9.5cm
D raw ground paste 10.4cm
Conclusion
A firm conclusion is not applicable to this experiment.
Cells can break down hydrogen peroxide because hydrogen peroxide is
poisonous and is a product when the liver breaks down waste therefore
enzymes (catalase) must be made in order to deal with it.
When the enzyme catalase is added to hydrogen peroxide, oxygen gas is
5. Two or more samples may be applied to each plate if they are kept
5. A second test tube was then filled with water and placed in a test
...imary stain and not pick up the counterstain. Other human errors could have affected the results such as not inverting the plate before putting it into incubation would not allow for bacterial growth. Not pipetting the tube up and down to mix the bacteria that settled at the bottom of the tube before starting the Gram Stain would not allow for an accurate reading because there wouldn’t be many bacteria on the slide. Passing the slide over the bunsen burner too many times, hence killing the bacteria and not allowing for a Gram Stain. If this experiment had to be redone, one improvement would be to allow for more that one plate without a point deduction. Unexpected human errors might interfere with person’s results. Having more than one plate will allow for more accuracy in the results or allow for a person to determine were they went wrong during the experiment.
3.) Divide your 30g of white substance into the 4 test tubes evenly. You should put 7.5g into each test tube along with the water.
The procedure of the lab on day one was to get a ring stand and clamp, then put the substance in the test tube. Then put the test tube in the clamp and then get a Bunsen burner. After that put the Bunsen burner underneath the test tube to heat it. The procedure of the lab for day two was almost exactly the same, except the substances that were used were different. The
Observations: Liver and Potatoes will be placed in hydrogen peroxide in order to observe the reactions due to the enzyme, catalase, found in both.
4. Put each group of potato discs in one of the 6 test tubes and watch
2. In the large beaker, put water and boil it completely. After that, remove the beaker from heat. 3. Sample tubes (A-D) should be labeled and capped tightly.
The Effect of pH on Enzyme Activity. pH is a measure of the concentration of hydrogen ions in a solution. The higher the hydrogen ion concentration, the lower the pH. Most enzymes function efficiently over a narrow pH range. A change in pH above or below this range reduces the rate of enzyme reaction. considerably.
the gain or loss of water when samples of the tissue are placed in a
== § Test tubes X 11 § 0.10 molar dm -3 Copper (II) Sulphate solution § distilled water § egg albumen from 3 eggs. § Syringe X 12 § colorimeter § tripod § 100ml beaker § Bunsen burner § test tube holder § safety glasses § gloves § test tube pen § test tube method = == = =
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If a person in a lab is going to look at several blood samples. This person sees that all of the ...