Identifying an Unknown Analgesic

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Identifying an Unknown Analgesic

Objectives:

1. To identify a unknown analgesic

2. To identify the analgesic you will be given 4 known analgesics

compounds. Each of which can be separated using by Thin Layer

Chromatography.

3. Using thin layer chromatography the unknown analgesic will be

compared to the 4 known analgesics.

Spotting of the sample

1. Prepare a microcapillary tube for each analgesic and each standard

2. Immerse the small end of the tube into the sample vial until some

of the sample is

drawn into the tube.

3. Very gently press the small end of the tube onto the plate about

one cm from the

bottom of the plate. Keep the spots small and concentrated by

applying the

sample 5 times and allowing the spot to dry between applications.

Additional spots may be required for the ibuprofen standard in

order to see its spot clearly.

4. Repeat this procedure for each of the standards available.

5. Two or more samples may be applied to each plate if they are kept

one cm apart.

Mark the position of the spots lightly in PENCIL and be sure to

keep a record of

which spot represents each product.

Development of the TLC plates

1. Prepare a developing chamber by adding 15-20 ml of solvent

2. Place the TLC plates in the chamber so that they do not touch.

Allow the

solvent to rise to within one cm of the top of the plates.

3. Remove the plates, mark the solvent front using pencil, and allow

them to dry.

4. Visualize the spots by illumination under a UV lamp.

5. Trace around each spot with a pencil and then measure the distance

traveled by

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