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Recommended: Essay about tetanus
Introduction
Clostridium tetani more commonly known as Tetanus is an acute infectious disease. Tetanus is not contagious and is a neurotoxin produced by Clostridium tetani. It is characterized by muscular spasms that mainly involve the voluntary muscle groups. Tetanus can affect horses, goat, swine, cat, dog, sheep, cattle and many other domestic animals. In cattle the disease is relatively rare, but in some cases can spread through a herd causing substantial economic loss. Clostridium tetani is a gram-positive anaerobic bacteria that can be found in the soil, intestines of many animals and therefore it is also naturally found in the feces of most animals. Tetanus is more common during warmer months and less common during the colder months when the ground is frozen. Tetanus enters the body through a wound, most commonly in cattle via a castration site or umbilical location of a newborn calf.
History
Tetanus is one of the earliest recognized diseases and was known and described by Hippocrates in the 6th century B.C. (Gibbons et. al, 1970) In 1844 scientist were able to produce tetanus in a rabbit. They had taken material from a human that died from the disease and injected into the rabbit. (Jensen et. al, 1973) Soon after the disease was produced in guinea pigs, mice and rats, this was accomplished by inoculating them with garden soil. (Gibbons et. al, 1970) It was not until 1889 that Clostridium tetani was cultured.
Etiology
Clostridium tetani is gram-positive, anaerobic bacteria that have poor motility and the ability to bear spores. The spores are strongly resistant to heat, light and drying and may survive in soil and feces for months to years. When the spores are allowed to be in contact with direc...
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...g the skin. Vaccinations that protect against Clostridium tetani are also available.
Treatment
Without treatment the affected cattle may die in three to ten days and even so the mortality rate it sixty percent. In order to treat these animals they must be tranquilized. Penicillin or another antibiotic must be administered to halt bacterial multiplication and production on toxins. (Thomas et. al, 2009) Since the animals muscles will be in spasm and very rigid they will be unable to eat or drink, treatment must also include means of supportive care. This may be accomplished by giving intravenous fluids or force feeding via stomach tube. The supportive treatment must be maintained until the signs of muscle spasms have diminished and the animal regains control over its body. This may take as long as one to four weeks. (Thomas et. al, 2009)
Clostridium difficile, otherwise known as C. diff, is a species of spore-forming, anaerobic, gram-positive bacteria that is known to cause watery diarrhea. 1 The genus name, Clostridium refers to the spindle shape of the organism while Difficile means difficult in Latin due to the fact that this organism thrives in unfavorable conditions and is very difficult to isolate.4 The incidence of getting CDI has increased over the years due to new strains of increased toxin production of the bacteria and increased resistance to antibiotics.2 It is a gastrointestinal infection, and the most common cause of infectious diarrhea.1 C. difficile was first identified in the feces of healthy newborns back in the 1930’s and by 1935, it was considered normal flora. 2 During 1974, researchers conducted that about 21% of patients that were treated with an antibiotic called clindamyacin reported diarrhea and about 10% of them reported to have conducted pseudomembranous colitis as a side effect of this treatment. 2 It was in 1978 where C. diff had been known to cause anti-biotic associated diarrhea and pseudomembranous colitis. 2 It is known to form spores that resist many disinfectants; it also survives for several months on different surfaces.1 It is a common form of a nosocomial infection and the prevalence of becoming infected with C. diff is about 0-15% in a health care setting. 3 The spores survive well in environments such as soil, water and animals and is distributed worldwide. 4 CDI produces two toxins (Toxin A and B), which are cytotoxic and cause tissue necrosis.4
The purpose of this study is to identify an unknown bacterium from a mixed culture, by conducting different biochemical tests. Bacteria are an integral part of our ecosystem. They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species. These biochemical tests are process of elimination that relies on the bacteria’s ability to breakdown certain kinds of food sources, their respiratory abilities and other biochemical conditions found in nature.
A remarkable breakthrough in medicine occurred in the late 1800s through the work of Louis Pasteur. Pasteur's experiments showed that bacteria reproduce like other living things and travel from place to place. Using the results of his findings, he developed pasteurization, which is the process of heating liquids to kill bacteria and prevent fermentation. He also produced an anthrax vaccine as well as a way to weaken the rabies virus. After studying Pasteur's work, Joseph Lister developed antisepsis, which is the process of killing disease-causing germs. In 1865 before an operation, he cleansed a leg wound first with carbolic acid, and performed the surgery with sterilized (by heat) instruments. The wound healed, and the patient survived. Prior to surgery, the patient would've needed an amputation. However, by incorporating these antiseptic procedures in all of his surgeries, he decreased postoperative deaths. The use of antiseptics eventually helped reduce bacterial infection not only in surgery but also in childbirth and in the treatment of battle wounds. Another man that made discoveries that reinforced those of Pasteur's was Robert Koch. Robert Koch isolated the germ that causes tuberculosis, identified the germ responsible for Asiatic cholera, and developed sanitary measures to prevent disease. (1)
As stated in Chan-Tack and Bartlett’s article Botulism, “The incidence of foodborne botulism is approximately 24 cases per year. The incidence of wound botulism is 3 cases per year. The incidence of infant botulism is 71 cases per year, with a mean age of 3 months.” (2010). In addition, in merely fifteen percent of the Clostridium botulinium outbreaks are the toxin type undetermined. The first case descriptions of botulism were reported by Dr. Justinus Kerner, a German physician, in 1822. He had conducted experiments on himself and laboratory animals, which gave him this case findings (Taillac, & Kim, 2010).
Clostridium perfringens is a gram-positive spore-forming bacillus involved in foodborne illness and wound infection. It is an obligate anaerobe and the only member of the genus Clostridium that is non-motile. This microorganism is normally present in soil and decaying vegetation and is an inhabitant of animal and human intestines. According to the Centers for Disease Control and Prevention, C. perfringens is one of the most common sources of foodborne illness in the United States, being the cause of an estimated 1 million cases each year. However, it is also prevalent worldwide. This bacterium has the shortest reported generation time of any organism at 6.3 minutes in thioglycollate medium, making it particularly virulent after initial inoculation.
Bacillus anthracis or anthrax is bacteria that infect thousands of people a year worldwide. Mostly found in the hinds and hair of grazing animals like cattle and sheep, the bacteria can stay dormant for years until contacted. Contact can occur through broken skin, eating infected meat, or inhaling infected dust from hides through the mouth or nostrils. Normal contact through the skin begins forming malignant skin ulcers that are dark black in appearance. If left untreated, the blisters lead to blood poisoning and eventually death. Eating contaminated meat not fully cooked can cause intestinal infection also leading to death. Surprisingly, many people survive contact of the skin and very few cases of intestinal infection exist. However, inhalation of infected dust or spores are much more deadly because the symptoms resemble a common cold...
Tetanus: acute infectious disease of the central nervous system caused by the toxins of Clostridium Tetani.
However, health concerned organizations want to ban the use of these products due to the increasing fears that they can cause harm to the consumers. For over 50 years, antibiotics have been added to the food of animals such as poultry, cattle and pigs. The main purpose for doing so is to lower the risk of disease in animals. Farm animals are housed together in overcrowded areas, which are very dirty. The hygiene level can get to such a poor state that they are often in contact with their own excreta as well as excreta of the other animals they are housed with and because of tight single air space they share, the likelihood of catching diseases from one another is further increased and very often a whole heard can be infected at one time.
TB is a significant zoonotic disease (a disease of animals which can also infect humans).
In this method, living spores which are resistant to whichever sterilizing agent is being tested are prepared in either a self contained system, such as dry sp...
Anthrax is a bacterial disease caused by bacteria called Bacillus anthracis (buh-SIL-us an-THRAY-sus). The Bacillus anthracis can exist in two different forms: favorable form, when the bacteria are rod-shaped and can grow and divide, or the unfavorable form, when they form a very resistant dormant spore that helps them survive extreme environmental conditions. Like other bacteria, Bacillus anthracis is very small, only about 1 by 9 micrometers in size. It is Gram-positive, which makes the bacteria purple-pinkish rods, with a relatively long lifespan of over 70 years. Bacillus anthracis feed on iron, oxygen, water, and nutrients to survive and reproduce. Bacillus anthracis need a favorable environment, but can reproduce in many different ways, either through spore germination, asexual bacterial reproduction, or formation of new spores. Herbivore animals such as cattle, sheep, and goats are the primary target of these bacteria and can be infected when they breathe in or ingest spores in contaminated soil, plants, or water. These elliptical shaped spores have thick and layered walls which make it resistant to heat, dryness, ultraviolet light, and gamma radiations. These spores initially develop inside the rod-shaped form, causing it to be named endospore. Because the bacteria is zoonosis, which means that it affects domestic and wild animals primarily, it is very rare for people to be infected by anthrax, but it is theoretically possible when the spores get into the body either through coming in contact with infected animals or contaminated animal products. Even though people can be infected with anthrax through animal contacts, it is not contagious, meaning that it cannot be transmitted from a person to person. After the spores get ...
years, and there is still no cure, but at the peak of its devastation in the United
T. pallidum is highly sensitive to oxygen and has a decreased ability to survive when not in human body temperature environments 1. The mode of transmission is through sexual contact or vertical transmission from the mother to the fetus. T. pallidum lacks the lipopolysaccharide which is the endotoxin normally present in gram negative bacteria1. The bacterium does produce many lipoproteins which are thought to prompt the inflammatory mediators through the recognition of toll-like receptors1. T. pallidum has a virulence factor of being highly motile due to its ability to propel itself forward by rotating on a longitudinal axis1. The spirochetes easily penetrate the skin or mucosal membranes and spread throughout the lymph nodes and then the blood circulation, affecting many parts in the body1.
It is estimated that over one-half of the antibiotics in the U.S. are used in food animal production. The overuse of antimicrobials in food animal production is an under-appreciated problem. In both human and veterinary medicine, the risk of developing resistance rises each time bacteria are exposed to antimicrobials. Resistance opens the door to treatment failure for even the most common pathogens and leads to an increasing number of infections. The mounting evidence of the relationship between antimicrobial use in animal husbandry and the increase in bacterial resistance in humans has prompted several reviews of agricultural practices by scientific authorities in a number of countries, including the US.