Spinach Chloroplast Lab

The isolation of the chloroplast from the spinach which is the control and the red cabbage, besides the isolation, then the photosynthesis assay was performed to determine the measurement of the effect of light on chloroplast. Base on the isolation of chloroplast of spinach and the different measurements of the effect of light, will the red cabbage have the same effect of light on the chloroplasts? In the first part, the chloroplast of the red cabbage and the spinach will be isolated. Then, the red cabbage or spinach homengate will be poured through the cheesecloth into the cold beaker. Pour the remaining filtrate onto a conical tube and centrifuge at low speed. Next, pipet some supernatant (S1) into a microcentrifuge tube and keep the tube on ice.

Pipet chill 0.5M sucrose onto the chloroplast pellet. Use a wooden stick to mix the pellet with the sucrose until it is completely resuspended. Pipet the resuspended pellet into microcentrifuge tube labeled “P2. and keep the tube on ice. For the next steps, pipet DCPIP mix into cuvettes 1- 6. And pipet deionized water into cuvette Pre-warm the cuvettes for 5 minutes in a 37C water bath and set the spectrophotometer to 620 nm. Measure the initial absorbance of cuvettes 1-6 using cuvette 7 as the blank. Place cuvettes 2, 4, and 6 in a dark, 37C incubator. Place cuvettes 1, 3 and 5 in a test tube rack with a 100W light bulb. Read the absorbance of cuvettes 3 and 5 EVERY MINUTE for the next 10. Measure the absorbance of the dark incubated cuvettes 2, 4 and 6 after 15 minutes. After conducting the isolation of chloroplast and the photosynthesis assay of both plant sources, out of red cabbage and spinach, the spinach had a constant decrease of absorbance compared to red cabbage were it varied as the minutes passed by. Also as a result, for the red cabbage there was not a noticeable pellet like their was in the